Acetylsalicylic-acid (ASA) regulation of osteo/odontogenic differentiation and proliferation of human dental pulp stem cells (DPSCs) in vitro

Vukovic, Mladen; Lazarević, Miloš; Trišić, Dijana; Karišik, Milica Jakšić; Ilić, Branislav; Andrić, Miroslav; Jevtić, Bojan; Roganović, Jelena; Milašin, Jelena

doi:10.1016/j.archoralbio.2022.105564

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Teeth were immediately transported to the laboratory and further processed under sterile conditions. Teeth surfaces were thoroughly rinsed with phosphate buffered saline solution (Thermo Fisher Scientific, Waltham, MA, USA), and DPSCs were isolated and characterized as previously described. , Briefly, the dental pulp was extracted with an endodontic instrument after having exposed the pulp chamber by crushing the tooth with a sterile clamp. Tissues were cut into 1 mm 3 pieces and transferred into culture medium [Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimitotic solution (all from Thermo Fisher Scientific, Waltham, MA, USA)].…”

Section: Methodsmentioning

confidence: 99%

Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity

Lazarević,

Ignjatović,

Mahlet

et al. 2024

ACS Appl. Nano Mater.

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Hydroxyapatite (HAp) has been the main protagonist in the quest for an ideal biomaterial for regenerative medicine over the last half a century. To control its properties, this material has commonly been doped with chemical elements other than its natural stoichiometric constituents: Ca, O, P, and H. Here, we report on the first analysis of the biological response to germanium-doped hydroxyapatite (Ge-HAp). Cytotoxicity, osteogenic differentiation induction, and colony formation potential were measured on dental pulp stem cells, while the antimicrobial effect was assessed against Gram-negative Escherichia coli, Grampositive methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans. All analyses were run in comparison to Ge-free HAp. Cell viability was inversely dependent on the nanoparticle concentration and incubation time. Adding Ge to HAp reduced cell viability relative to HAp after 24−72 h incubation periods, but the effect was reversed after longer incubations, when the viability of cells treated with low doses of Ge-HAp exceeded that of HAptreated cells and became comparable with control culture. Both HAp and Ge-HAp induced mineral formation in the cell culture, but the effect was more pronounced for Ge-HAp. Likewise, relative to both control cells and cells exposed to HAp, Ge-HAp upregulated the expression of all three osteogenic markers analyzed, namely, alkaline phosphatase, RUNX2, and osteocalcin, exerting the key influence on osteogenesis in its early, differentiation stage. The colony formation capacity of stem cells, however, was impaired by HAp and even more so by Ge-HAp. The antimicrobial effect was dependent on the microorganisms tested. Thus, whereas the antimicrobial activity was absent against E. coli, it was evident against MRSA and C. albicans. While the antibacterial activity against MRSA was weakened by the addition of Ge to HAp, the antimycotic activity against C. albicans was intensified with the addition of Ge. These findings demonstrate a significant potential of Ge-doped HAp nanoparticles in regenerative medicine due to their pronounced biocompatibility, osteoinductivity, and antimicrobial activity.

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Section: Methodsmentioning

confidence: 99%

Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity

Lazarević,

Ignjatović,

Mahlet

et al. 2024

ACS Appl. Nano Mater.

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“…Teeth were delivered immediately to the laboratory, where they underwent additional sterile processing. Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, Waltham, MA, USA) was used to properly clean the surfaces of the teeth before DPSCs were isolated and characterized as previously described [ 39 ]. In brief, the dental pulp was removed using an endodontic file after the pulp chamber was exposed by crushing the tooth with a sterile clamp.…”

Section: Methodsmentioning

confidence: 99%

“…After 24, 48 and 72 h, the cell cultures’ incubation was halted by discarding the used medium. Each membrane was transferred to the new 96-well plate and each well was filled with complete medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 0.5 mg/mL) (from Sigma-Aldrich, St. Louis, MI, USA) and incubated for an additional 4 h [ 39 ]. After discarding the supernatant, the formazan crystals were broken down in 100 μL of dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MI, USA) by vigorous shaking for 15 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite

et al. 2023

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Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating bone growth, bacterial contamination still remains one of the disadvantages of membrane use. Thus, we assessed the biocompatibility and osteogenic and antibacterial properties of a collagen membrane (OsteoBiol) modified with chitosan (CHI) and hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) were performed for membrane characterization. Biocompatibility was assessed on dental pulp stem cells (DPSCs) by an MTT assay, while the osteogenic effect was assessed by an ALP activity assay and qPCR analysis of osteogenic markers (BMP4, ALP, RUNX2, and OCN). Antimicrobial properties were investigated by counting colony-forming units (CFUs) of Streptococcus mitis, Porphyromonas gingivalis, and Fusobaterium nucleatum on membranes and in the surrounding medium. Membranes showed no cytotoxicity. ALP activity was higher and ALP, BMP4, and OCN genes were up-regulated in DPSCs on modified membranes compared to unmodified membranes. The CFUs were reduced on modified membranes and in the medium. Modified membranes showed great biocompatibility and a high osteoinductive effect. Additionally, they showed antimicrobial and antibiofilm effects against periopathogens. It can be concluded that the incorporation of CHI and hydroxyapatite nanoparticles in collagen membranes may be advantageous to promote osteogenesis and reduce bacterial adhesion.

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3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/odontogenic differentiation

López-García,

Aznar-Cervantes,

Pagán

et al. 2024

Dental Materials

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Acetylsalicylic-acid (ASA) regulation of osteo/odontogenic differentiation and proliferation of human dental pulp stem cells (DPSCs) in vitro

Cited by 5 publications

References 28 publications

Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity

Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity

Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite

3D Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/odontogenic differentiation

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