Acetylene inhibition of nitrous oxide reduction and measurement of denitrification and nitrogen fixation in soil

Yoshinari, Tadashi; Hynes, Russell K.; Knowles, Roger

doi:10.1016/0038-0717(77)90072-4

Cited by 496 publications

(208 citation statements)

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“…ARA was measured four times during 40 days in the same microcosms. Acetylene is known to inhibit the (methane and ammonium) monooxygenases of methanotrophs and other alkane-and ammonium-utilizing bacteria (De Bont and Mulder 1976), as well as the nitrous oxide reductase of denitrifiers (Yoshinari et al 1977).…”

Section: Methodological Considerationsmentioning

confidence: 99%

Effects of nitrogen fertilization on diazotrophic activity of microorganisms associated with Sphagnum magellanicum

et al. 2016

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Background and aims In pristine ombrotrophic Sphagnum-dominated peatland ecosystems nitrogen (N) is often a limiting nutrient, which is replenished by biological N 2 fixation and atmospheric N deposition. It is, however, unclear which impact long-term N deposition has on microbial N 2 fixing activity and diazotrophic diversity, and whether phosphorus (P) modulates the response. Therefore, we studied the impact of increased N deposition and N depletion on microbial N 2 fixation and diazotrophic diversity associated with the peat moss Sphagnum magellanicum, and their interaction with P availability. Methods Nitrogenase activities of S. magellanicumassociated microorganisms were determined by acetylene reduction assays (ARA) and 15 N 2 tracer methods on mosses from two geographically distinct locations with different N deposition histories, high or low N deposition, and in samples depleted in N (grown 3 years in the greenhouse) versus recent field samples. The short-term response to increased N deposition was tested for mosses differing in N and P fertilization histories. In addition, diversity of diazotrophic microorganisms was assessed by nifH gene amplicon sequencing of N-depleted mosses. Results We showed distinct and persistent differences in diazotrophic communities and their activities associated with S. magellanicum from sites with high versus low N deposition. Initially, diazotrophic activity was six times higher for the low N site. During incubation and repeated ARA, however, this activity strongly decreased, while it remained stable for the high N site. Activity for the high N site could not be increased by long-term experimental N deprivation. Short-term, experimental N application had an inhibitory effect on N 2 fixation for both sites, which was not observed in mosses with high indirect P availability. Conclusions We conclude that although N deposition negatively affects N 2 fixation as also shown in previous studies, long-term effects of N deprivation on the diazotrophic activity and community are more complex. Furthermore, our results indicated that P availability might be an important factor in modulating the response of Sphagnum-associated diazotrophs to N deposition.

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Section: Methodological Considerationsmentioning

confidence: 99%

Effects of nitrogen fertilization on diazotrophic activity of microorganisms associated with Sphagnum magellanicum

et al. 2016

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“…Dynamics of N 2 O accumulation and reduction were determined by anaerobic incubation with and without C 2 H 2 (Yoshinari et al, 1977). Non-spiked control soil and soil treated with EC 50 concentration of pyrene (concentration of pyrene in soil causing 50% inhibition of denitrification activity; denoted as EC50 treatment) were prepared for the dynamic experiment.…”

Section: Time-course Of N 2 O Accumulation and Reductionmentioning

confidence: 99%

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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a b s t r a c tToxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0e500 mg kg À1 ) followed by doseeresponse and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC 50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC 50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs.

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“…In parallel with the measurements in control bottles (without glucose and ammonium sulfate addition), 2 mg C g-l of glucose and 0.2 mg N g-l of ammonium sulfate were added to other bottles, and the rates of CO 2 production were determined in the same manner. Besides CO 2 production, the rate of denitrification under anaerobic conditions was determined for the samples with the same moisture content by using the acetylene inhibition technique (Yoshinari et al 1977). In this experiment, 0.1 g N kg-1 of potassium nitrate was added to the samples, and the partial pressure of acetylene in the head space was adjusted at 0.5 kPa.…”

Section: Methodsmentioning

confidence: 99%

Changes in gross rates of nitrogen transformations in soil caused by conversion of paddy fields to upland fields

Nishio¹,

Sekiya²,

Toriyama³

et al. 1994

Soil Science and Plant Nutrition

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Acetylene inhibition of nitrous oxide reduction and measurement of denitrification and nitrogen fixation in soil

Cited by 496 publications

References 19 publications

Effects of nitrogen fertilization on diazotrophic activity of microorganisms associated with Sphagnum magellanicum

Effects of nitrogen fertilization on diazotrophic activity of microorganisms associated with Sphagnum magellanicum

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Changes in gross rates of nitrogen transformations in soil caused by conversion of paddy fields to upland fields

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