Acetylcholinesterase inhibitors neostigmine and physostigmine inhibit induction of alpha-amylase activity during seed germination in barley, Hordeum vulgare var. Jyoti

Beri, Veena; Gupta, Rajendra

doi:10.1016/j.lfs.2007.02.018

Cited by 28 publications

(19 citation statements)

References 13 publications

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“…Measurement of α-amylase activity in germinating seeds is a classical bioassay for determining the GA level (Beri & Gupta, 2007). In the present study, low concentrations of IAA (i.e., 5 and 10 µg/mL) promoted the induction of α-amylase in germinating durum wheat seeds, perhaps, because the exogenous IAA stimulated GA biosynthesis.…”

Section: Discussionmentioning

confidence: 54%

“…During seed germination in wheat, barley and other graminaceous species, gibberellic acid (GA) is formed in the embryo and transferred to the aleuronic layer, where it induces the synthesis of α-amylase (Beri & Gupta, 2007). Measurement of α-amylase activity in germinating seeds is a classical bioassay for determining the GA level (Beri & Gupta, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The reaction medium was incubated for 10 min at room temperature (22 ± 2 °C), then the reaction was terminated by adding 1 mL of a 0.1% iodine reagent (6 g potassium iodide, 0.6 g iodine in 1 L of 0.05 N HCl) and 3 mL of 0.05 N HCl. The absorbance was measured at 620 nm and the decrease in the absorbance relative to the blank was considered as α-amylase activity (Beri & Gupta, 2007).…”

Section: Bacteria Strains and Growth Conditionsmentioning

confidence: 99%

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Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Tabatabaei

Ehsanzadeh

Etesami

et al. 2016

Span. j. agric. res.

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The role of plant-associated bacteria in plant physiology and metabolism is well documented, but little has been known about the roles played by Pseudomonas in durum wheat (Triticum turgidum L. var durum) growth and development. An in vitroexperiment was conducted to observe the effect of the inoculation of four indole-3-acetic acid (IAA)-producing Pseudomonas isolates and exogenous IAA on seed germination traits and α-amylase activity of durum wheat. The results showed inoculation with all bacterial isolates led to a decrease in the germination percent, although the extent of the depression varied with the isolate. A significant relationship between concentrations of bacterial IAA and the germination inhibition percent in durum wheat seeds by different bacteria strains was observed. The results of this assay showed the effect of bacterial isolates on α-amylase activity after six and 8 days of inoculation was significant, while effect of these isolates on α-amylase activity after two and 4 days of inoculation was not meaningful. In addition, the exogenously applied IAA displayed a concentration-dependent effect on seed germination attributes and α-amylase activity, consistent with the possibility that the inhibitory effect of bacterial inoculation on seed germination was in consequence of bacteria-produced IAA. Therefore, it may suggested that the inhibitory role of IAA in seed germination and α-amylase activity should be taken into account during the screening of IAA-producing Pseudomonas isolates for durum wheat growth promoting agents.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Bacteria Strains and Growth Conditionsmentioning

confidence: 99%

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Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Tabatabaei

Ehsanzadeh

Etesami

et al. 2016

Span. j. agric. res.

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“…Notably, it was previously shown that the application of AChE inhibitors neostigmine and physostigmine to barley seeds repressed germination. 6 Thus, it appears that genes responsible for the biosynthesis, sensing, and degradation of ACh in Arabidopsis is localized primarily to the seed in Arabidopsis, while root is also the site that is capable of sensing exogenously applied ACh.…”

Section: Ach Does Not Significantly Affect Germination Rates Of Arabimentioning

confidence: 99%

“…1,6 Moreover, previous pharmacological studies indicate that ACh seems to be involved in various physiological processes including, but not limited to, the control of stomatal aperture, gravitropism, and root development. 7,8,9 However, despite the general occurrence of ACh across kingdoms, genes that are orthologous to known animal ChAT, AChE, or genes encoding muscarinic or nicotinic ACh receptors have not been successfully identified from plants or bacteria to date.…”

Section: Introductionmentioning

confidence: 99%

High-resolution mass spectrometry for detecting Acetylcholine in Arabidopsis

Murata

Watanabe

Sugahara

et al. 2015

Plant Signaling & Behavior

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Acetylcholine (ACh) was first identified a century ago, and has long been known as a neurotransmitter in animals. However, it has been shown recently that the occurrence of ACh is widespread among various non-animal species including higher plants. Although previous reports suggest that various plant species are capable of responding to exogenously applied ACh, the molecular basis for ACh biosynthesis and regulatory mechanisms mediated by endogenous ACh are largely unclear. This is partly because of the lack of conclusive data on the occurrence and the tissue specificity of ACh in plants. To this end, we performed various analyses including liquid chromatography electrochemical detection (LC-ECD), liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. The results, together with electrospray ionization-orbitrap Fourier transform mass spectrometry (ESI-orbitrap FT-MS) analysis provide strong evidence that ACh exists in Arabidopsis thaliana tissues. The results also showed that the level of ACh is highest in seed, followed by root and cotyledon. Moreover, exogenously applied ACh inhibited the elongation of Arabidopsis root hairs. These results collectively indicate that ACh exists primarily in seed and root in Arabidopsis seedlings, and plays a pivotal role during the initial stages of seedling development by controlling root hair elongation in Arabidopsis.

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Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

Chaâbene

Hakim

Rorat

et al. 2017

Enviro Toxic and Chemistry

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Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity was dose- and time-dependent. Likewise, H O production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks. However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element. Environ Toxicol Chem 2018;37:797-806. © 2017 SETAC.

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Acetylcholinesterase inhibitors neostigmine and physostigmine inhibit induction of alpha-amylase activity during seed germination in barley, Hordeum vulgare var. Jyoti

Cited by 28 publications

References 13 publications

Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

High-resolution mass spectrometry for detecting Acetylcholine in Arabidopsis

Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

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