Acetylcholine receptor-specific suppressive T-cell factor from a retrovirally transformed T-cell line.

Sinigaglia, Francesco; Gotti, Cecilia; Castagnoli, Paola Ricciardi; Clementi, Francesco

doi:10.1073/pnas.81.23.7569

Cited by 11 publications

(1 citation statement)

References 40 publications

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“…The specific suppressor activity of the LH8.105 cells on antilysozyme response was previously demonstrated, both in vitro and in vivo, by using the cell culture supernatant, the purified T-suppressor factor (20)(21)(22), or mRNA translation products (23). Similar studies have been performed to assess the suppressor activity of anti-acetylcholine-receptor response by clone LA41 (24). RadLV-VL3 (VL3) is a permanent T-cell line, chronically infected with highly oncogenic RadLV, and produces large amounts of infectious particles that can be recovered from the culture supernatant (25).…”

Section: Methodsmentioning

confidence: 65%

Rearrangement and expression of the alpha- and beta-chain genes of the T-cell antigen receptor in functional murine suppressor T-cell clones.

Santis¹,

Hsu²,

Adorini³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Two different antigen-speciflic radiation leukemia virus (RadLV)-transformed suppressor T-cell clones, LH8.105 and LA41, exhibiting anti-lysozyme and anti-acetylcholine-receptor suppressor activity, respectively, have been examined for rearrangement and expression of genes encoding the a and (3 chains of the T-cell receptor for antigen. LH8.105 cells express the T-cell-receptor polypeptides, as shown by specific immunoprecipitation. In both cell lines, potentially functional transcripts of a-and (3chain genes are detected by RNA blot analysis. These suppressor T-cell clones exhibit a-chain gene rearrangements, deletion of both alleles of the constant-region (C) gene segment CpIu, and rearrangement of the two alleles of Cp2 when analyzed by Southern blot hybridization. Restriction analysis suggests that the DNA rearrangement is beyond the secondjoining-region () minigene ofthe Jl2cluster. These results establish that at least some mouse suppressor T-cell clones, like helper and cytotoxic T lymphocytes, rearrange and transcribe the genes coding for the a and (3 chains of the antigen-specific T-cell receptor.Three different classes of T-cell-specific cDNA clones, a (1, 2), 13(3, 4), and y(5), have been isolated recently from human and mouse cDNA libraries. The a and (3 cDNA clones code for the a and 13 T-cell antigen-receptor subunits, respectively, as demonstrated by partial amino acid sequence data from the purified receptor (6, 7), whereas the protein product ofthe y gene has not been identified. The function of the y gene remains unclear, although its pattern of expression suggests a possible role during T-cell differentiation (8). The T-cell genes show sequence homology and genomic-organization similarities with immunoglobulin genes. The genomic sequences corresponding to these cDNA clones are rearranged and expressed specifically in T cells.The genomic organization of the /3 and y genes has been studied extensively. The 13 chain of the human and mouse antigen-specific T-cell receptor is encoded in variable (V), diversity (D), joining (J), and constant (C) gene segments.The chromosomal locus of the 3-chain genes appears to contain two linked constant genes (Cat and Cog), each associated with its own cluster of J genes (9-11). For the y class, three V,, gene segments and three Jy-C,, clusters have been identified in mouse DNA (12). The genomic organization of the a genes is still unknown, but the cDNA sequences suggest that Va-Ja and possibly Da gene segments are present and rearrange specifically in T cells to generate the transcriptionally active gene (1, 2). An obvious question is whether the three major antigen-specific T-cell subsets (cytotoxic, helper, and suppressor), which express on their membrane surface the a-f3 heterodimeric structure, use either Cat or Cp2in their rearranged genes. The data, so far, suggest that m human T-cell clones the Cr31 genes are rearranged and used by either cytotoxic, helper, or suppressor clones, excluding the possibility that Ca isotypes are subset-specific marker...

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Section: Methodsmentioning

confidence: 65%

Rearrangement and expression of the alpha- and beta-chain genes of the T-cell antigen receptor in functional murine suppressor T-cell clones.

Santis¹,

Hsu²,

Adorini³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Induction of murine T cell lymphoma expressing specific cytotoxic activity

1986

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The present work reports the establishment of an antigen-specific cytotoxic T cell lymphoma line after immortalization with a murine leukemia virus. Lymph node cells from mice bearing a transplanted syngeneic MCA sarcoma were infected in vitro with radiation leukemia virus and injected intrathymically into cogeneic recipient mice. Some lymphomas of donor origin were established as permanent continuous cell lines in vitro. One of them, NS8, expressed Thy-1.2, Lyt-1, Lyt-2 and peanut agglutinin surface markers. These cells were cytotoxic in vitro for the tumor cell line corresponding to the immunizing MCA sarcoma. No significant cytolytic activities against other syngeneic or allogeneic sarcomas or lymphomas, nor against the mastocytoma P815 were observed. After several months of in vitro propagation, the specificity of the cytotoxic activity had degraded and the level of Lyt-2 or peanut agglutinin receptors dropped. These characteristics were restored with a single in vivo passage. Thus, murine leukemia virus can be used to immortalize antigen-specific cytotoxic T cells.

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T lymphocyte recognition of acetylcholine receptor: Localization of the full T cell recognition profile on the extracellular part of the α chain of Torpedo californica acetylcholine receptor

et al. 1987

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A series of eighteen consecutive overlapping synthetic peptides, of uniform size and overlaps, which encompass the entire extracellular part (residues 1-210) of the Torpedo californica acetylcholine receptor alpha chain were examined in vitro for their ability to stimulate lymph node cells from acetylcholine receptor-primed C57BL/6 (H-2b), C3H/He (H-2k), SWR (H-2q) and SJL (H-2s) mice. The recognition sites (T sites) by acetylcholine receptor-primed lymph node cells from these mouse strains resided within six regions on the extracellular part of the alpha chain. Three of the regions recognized by T cells coincided with regions recognized by antibodies (i.e. B cells) and one of these three regions also coincided with an alpha-neurotoxin-binding region. It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has at least two sites which are recognized exclusively by T cells and to which no detectable antibody responses are directed.

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Acetylcholine receptor-specific suppressive T-cell factor from a retrovirally transformed T-cell line.

Cited by 11 publications

References 40 publications

Rearrangement and expression of the alpha- and beta-chain genes of the T-cell antigen receptor in functional murine suppressor T-cell clones.

Rearrangement and expression of the alpha- and beta-chain genes of the T-cell antigen receptor in functional murine suppressor T-cell clones.

Induction of murine T cell lymphoma expressing specific cytotoxic activity

T lymphocyte recognition of acetylcholine receptor: Localization of the full T cell recognition profile on the extracellular part of the α chain of Torpedo californica acetylcholine receptor

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