Acetylcholine Mediated Axon-Schwann Cell Relationships in the Squid Nerve Fiber

Villegas, Jorge

doi:10.1007/978-1-4684-3096-7_27

Cholinergic Mechanisms and Psychopharmacology

1978

DOI: 10.1007/978-1-4684-3096-7_27

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Acetylcholine Mediated Axon-Schwann Cell Relationships in the Squid Nerve Fiber

Jorge Villegas

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1979

1983

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“…The results show the presence of acetylcholine in the giant and small nerve fibers of the stellar nerve, and indicate that the Schwann cells of the giant nerve fiber have a high intracellular acetylcholine concentration, which is about 40 times that of the axoplasm. Preliminary results of this work have been reported elsewhere (VILLEGAS & JENDEN, 1977;VILLEGAS, 1977VILLEGAS, , 1978.…”

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Acetylcholine Content of the Schwann Cell and Axon in the Giant Nerve Fibre of the Squid

Villegas

Jenden

1979

Journal of Neurochemistry

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Abstract— Acetylcholine and choline were identified and their concentrations measured, by means of gas chromatography/mass spectrometry, in extracts obtained from nerve fibers of the hindmost stellar nerve of the squid Sepioteuthis sepioidea. These compounds were quantitated in samples of stellar nerve devoid of giant fiber, intact giant nerve fiber, extruded axoplasm, and axoplasm‐free giant nerve fiber sheaths. In 11 samples of stellar nerve devoid of giant fiber, weighing an average of 20.8 ± 2.3 mg (s.e.m.), 756 ± 91 pmol ACh and 8.65 ± 0.62 nmol of choline were found. The total ACh content of the largest fibre in this group (10 μm in diameter), for a 5 cm length of nerve, is in the order of 0.16 pmol. The average wet weights of a single giant nerve fiber (270‐420 μm in diameter) and its separate components (s.e.m.; in mg; number of fibers in parentheses) were: intact fiber, 4.58 ± 0.19 (25); extruded axoplasm, 3.38 ± 0.13 (20); sheaths, 1.21 ± 0.11 (16). The average ACh content per unit weight of sample was about 2‐3 times higher in the sheaths (5‐13 pmol‐mg−1) than in the axoplasm (2‐4 pmol mg−1), whereas the ACh concentrations estimated per unit volume of cellular water were about 40 times higher in the Schwann cell (107‐222 μm) than in the axon (2‐5μm). These experimental findings establish the presence of ACh in the giant nerve fiber of S. sepioidea. They also indicate the Schwann cells themselves as the main source for the release of ACh, responsible for their long‐lasting hyperpolarizations following the conduction of nerve impulse trains by the axon.

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confidence: 66%

Acetylcholine Content of the Schwann Cell and Axon in the Giant Nerve Fibre of the Squid

Villegas

Jenden

1979

Journal of Neurochemistry

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Simultaneous ultrastructural localization of serotonin and cholinesterases in Mytilus byssal retractor muscle (A.B.R.M.)

1983

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Ultrastructural localization techniques for cholinesterases (ChE) by the Karnovsky method, for monoamines by the technique of Wood, and Tranzer and Richards, and for 5HT by the technique of Wood, were performed on the anterior byssal retractor muscle of Mytilus. Simultaneous ultrastructural localization of 5HT and ChE was obtained by the use of the false neurotransmitter 5,6-DHT, followed by the method of Karnovsky for ChE. The glio-interstitial tissue presents a constant ChE activity, mainly localized on the plasma membrane, even in those processes which accompany tryptaminergic (5HT containing) neurites. Muscle cell membrane also reacts positively to the Karnovsky method. The tryptaminergic neurites themselves do not show any ChE activity; they contain dichromate reactive large (100 nm) dense cored vesicles. The presence of differentiated tryptaminergic neuromuscular junctions, suggested by other authors, is established. It was not possible to distinguish classes of nerve endings by the typology of their vesicular content. It is concluded that one can reasonably plan to study the effect of glial ChE inhibition on the physiology of the tryptaminergic (relaxing) response of the A.B.R.M. to nerve stimulation.

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Acetylcholine Mediated Axon-Schwann Cell Relationships in the Squid Nerve Fiber

Cited by 2 publications

References 21 publications

Acetylcholine Content of the Schwann Cell and Axon in the Giant Nerve Fibre of the Squid

Acetylcholine Content of the Schwann Cell and Axon in the Giant Nerve Fibre of the Squid

Simultaneous ultrastructural localization of serotonin and cholinesterases in Mytilus byssal retractor muscle (A.B.R.M.)

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