Acetylcholine esterase enzyme doped multiwalled carbon nanotubes for the detection of organophosphorus pesticide using cyclic voltammetry

Thakkar, Jinal B.; Gupta, Shweta; Prabha, C. Ratna

doi:10.1016/j.ijbiomac.2019.06.162

Cited by 50 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the inhibition rate of organophosphorus pesticides on enzymes also changes with time. In the first 10 min of the enzyme inhibition reaction, the inhibition rate of organophosphorus pesticides on the enzyme showed an upward trend, but after that, the inhibition rate tended to stabilize (Du, Ye, Cai, Liu, & Zhang, 2010; Thakkar, Gupta, & Prabha, 2019). Figure 3b shows the change of the inhibition rate of pesticides on enzymes with time.…”

Section: Methodsmentioning

confidence: 99%

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

Yang

et al. 2020

J Food Process Engineering

View full text Add to dashboard Cite

Different organophosphorus pesticides (OPs) normally vary in the level of toxicity to human health, but common electrochemical identification methods usually require complex electrode fabrication processes and high detection cost. To overcome these problems, a pesticide residues identification method based on multilayer paper-based microfluidic chip was proposed. The microfluidic chip integrates the enzyme inhibition and a label-free screen-printed carbon electrode (SPCE). Three different pesticides were selected as study objects, respectively, avermectin, phoxim, dimethoate. This chip realizes on-chip identification via analyzing impedance time-sequence spectrum data aroused by the enzyme inhibition during the 15 min period. A classification model based on principal component analysis (PCA) and support vector machines (SVMs) was established to evaluate these data. Furthermore, the real-world sample (lettuce) was tested to verify the feasibility of the proposed detection method. The results showed that the classification accuracy was achieved around 93% in multiple experiments. Therefore, the method has good stability and specificity in rapid identification of different pesticides. Practical Applications Identification of pesticide types is essential to human health, for this, impedance time-sequence spectrum data aroused by the enzyme inhibition during the 15 min period was used to establish a classification model to identity different types of pesticides. In this article, multilayer paper-based microfluidic chip and a label-free screenprinted carbon electrode (SPCE) was proposed without complicated operation steps and expensive cost, which provide a basis for establishment of the rapid pesticide discriminator.

show abstract

Section: Methodsmentioning

confidence: 99%

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

Yang

et al. 2020

J Food Process Engineering

View full text Add to dashboard Cite

show abstract

“…Inhibition of enzymatic reaction was also employed for the detection of other CWAs, such as organophosphate derivatives (OP). An interesting example is an electrochemical biosensor involving MWCNTs and acetylcholinesterase for the determination of paraoxon with an LOD value of 0.1 nM [130]. A sensitive amperometric acetylcholinesterase biosensor for OPs, using a 4,7-di (furan-2-yl) benzo [1,2,5] thiadiazole polymer, AgNPs and amine-functionalized rGO, was recently reported, as well.…”

Section: Chemical Warfare Agents (Cwas)mentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

View full text Add to dashboard Cite

This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

show abstract

“…Hence, nanomaterial-based systems have been developed to address these issues due to their promising properties. [28][29][30] Carbon nanotubes (CNTs), which are one-dimensional nanomaterials with a distinctive hexagonal shape, possess excellent electrical conductivity and can be used as electrode modiers to accelerate electrode responses, reduce overpotential, and facilitate electron transfer events. 31,32 However, the widespread use of CNTs has been constrained by their poor solubility in solvents and suboptimal dispersion.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical biosensor for detection of circulating tumor cells based on a highly efficient enzymatic cascade reaction

et al. 2023

View full text Add to dashboard Cite

show abstract

Acetylcholine esterase enzyme doped multiwalled carbon nanotubes for the detection of organophosphorus pesticide using cyclic voltammetry

Cited by 50 publications

References 30 publications

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

Pesticide residues identification by impedance time‐sequence spectrum of enzyme inhibition on multilayer paper‐based microfluidic chip

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Ultrasensitive electrochemical biosensor for detection of circulating tumor cells based on a highly efficient enzymatic cascade reaction

Contact Info

Product

Resources

About