Acetylation of polyamines in mouse brain: Subcellular and regional distribution

Ortíz, José G.; Giacobini, Ezio; Schmidt-Glenewinkel, T.

doi:10.1002/jnr.490090211

Cited by 11 publications

(1 citation statement)

References 33 publications

(18 reference statements)

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“…Intracellular concentrations of polyamines have been reported in the millimolar range for most tissues and organisms, but concentrations vary with cell type, health and growth cycles Tabor, 1976, 1984;Ortiz et al, 1983). Increasing concentrations of putrescine, cadaverine and spermine appeared to be correlated with the degree of decomposition of certain aquatic animals (Mietz and Karmas, 1978) and are therefore probably prevalent in the aquatic environment where they are presumably encountered by teleosts.…”

Section: Polyamines Are Essential Naturally Occurring Compoundsmentioning

confidence: 99%

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Rolen

Sorensen

Mattson

et al. 2003

Journal of Experimental Biology

109

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SUMMARY Electrophysiological responses of goldfish olfactory receptor neurons(ORNs) and goldfish behavioral responses to polyamines were investigated in vivo. Electro-olfactogram (EOG) recordings indicated that polyamines (putrescine, cadaverine and spermine) are potent olfactory stimuli for goldfish with estimated electrophysiological thresholds of 10–8–10–7 mol l–1,similar to that for L-arginine, the most stimulatory amino acid. Although thresholds were similar, the magnitude of the EOG responses to intermediate(10–5–10–4 mol l–1)and high (10–3 mol l–1) concentrations of polyamines dwarfed the responses to amino acids and related single amine containing compounds (amylamine and butylamine). The EOG responses to 0.1 mmol l–1 putrescine, cadaverine and spermine were, respectively,4.2×, 4.3× and 10.3× the response of the standard, 0.1 mmol l–1 L-arginine. Electrophysiological cross-adaptation experiments indicated that polyamine receptor sites are independent from those to L-amino acids (alanine, arginine, glutamate, lysine, methionine and ornithine), bile salts (sodium taurocholate and taurolithocholate), the single amine containing compounds (amylamine and butylamine) and ATP. Further, the cross-adaptation experiments revealed the existence of independent receptor sites for the different polyamines tested. Pharmacological experiments suggested that polyamine odorant transduction does not primarily involve the cyclic AMP and IP3 second messenger pathways. Behavioral assays indicated that polyamines are attractants that elicit feeding behavior similar to that elicited by L-amino acids.

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Section: Polyamines Are Essential Naturally Occurring Compoundsmentioning

confidence: 99%

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Rolen

Sorensen

Mattson

et al. 2003

Journal of Experimental Biology

109

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Metabolism of cadaverine and pipecolic acid in brain and other organs of the mouse

Nomura

Schmidt-Glenewinkel

Giacobini

et al. 1983

J of Neuroscience Research

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Cadaverine and pipecolic acid metabolism was investigated in vitro in several organs of the mouse by measuring 14CO2 formation from labeled precursors. The liver showed the highest formation of 14CO2 from [1,5-14C]-cadaverine, whereas brain demonstrated a much lower formation. Anaerobiosis or inhibition of monoamineoxidase (MAO) activity significantly reduced 14CO2 formation in every organ, but inhibition of diamine oxidase (DAO) activity had no effect in brain and kidney. Piperidine was formed from cadaverine in vitro only in the large intestine and its content. This formation is probably of bacterial origin. Under a variety of experimental conditions we were unable to demonstrate any formation of piperidine in brain from cadaverine. Biosynthesis in vitro of [3H]-piperidine from D,L-[3H]-pipecolic acid was very low in brain and kidney. With the exception of brain and kidney, no other organs showed any formation of [3H]-piperidine. Neither MAO nor DAO inhibition influenced [3H]-piperidine formation in the large intestine with its content. Following 1 hr incubation at 37 degrees C under aerobic conditions, the levels of [14C]-pipecolic acid and [3H]-piperidine recovered from mouse brain homogenate did not indicate any significant degradation of these two substances. Our results suggest that under in vitro conditions, cadaverine is not a precursor of piperidine in brain, liver, heart, and kidney and that only very low levels of piperidine can be formed from pipecolic acid in brain. Outside the brain, formation of piperidine from pipecolic acid is detectable only in kidney and in the content of the large intestine. The latter is probably of bacterial origin. Our results do not support previous findings from other authors on an endogenous origin of piperidine in brain from cadaverine and pipecolic acid, and they suggest that a) cadaverine is not a precursor of piperidine in brain, b) the conversion of pipecolic acid into piperidine in the brain does not constitute a major metabolic pathway, and c) the main source of piperidine in the CNS may be of nonneural origin.

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Rectification of the Olfactory Cyclic Nucleotide-Gated Channel by Intracellular Polyamines

Lynch

1999

Journal of Membrane Biology

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Polyamine-induced inward rectification of cyclic nucleotide-gated channels was studied in inside-out patches from rat olfactory neurons. The polyamines, spermine, spermidine and putrescine, induced an 'instantaneous' voltage-dependent inhibition with Kd values at 0 mV of 39, 121 micron and 2.7 mM, respectively. Hill coefficients for inhibition were significantly < 1, suggesting an allosteric inhibitory mechanism. The Woodhull model for voltage-dependent block predicted that all 3 polyamines bound to a site 1/3 of the electrical distance through the membrane from the internal side. Instantaneous inhibition was relieved at positive potentials, implying significant polyamine permeation. Spermine also induced exponential current relaxations to a 'steady-state' impermeant level. This inhibition was also mediated by a binding site 1/3 of the electrical distance through the pore, but with a Kd of 2.6 mM. Spermine inhibition was explained by postulating two spermine binding sites at a similar depth. Occupation of the first site occurs rapidly and with high affinity, but once a spermine molecule has bound, it inhibits spermine occupation of the second binding site via electrostatic repulsion. This repulsion is overcome at higher membrane potentials, but results in a lower apparent binding affinity for the second spermine molecule. The on-rate constant for the second spermine binding saturated at a low rate (approximately 200 sec(-1) at +120 mV), providing further evidence for an allosteric mechanism. Polyamine-induced inward rectification was significant at physiological concentrations.

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Acetylation of polyamines in mouse brain: Subcellular and regional distribution

Cited by 11 publications

References 33 publications

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Metabolism of cadaverine and pipecolic acid in brain and other organs of the mouse

Rectification of the Olfactory Cyclic Nucleotide-Gated Channel by Intracellular Polyamines

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