Acetyl-coenzyme A in patulin biosynthesis

Bassett, Emmett W.; Tanenbaum, Stuart W.

doi:10.1016/0006-3002(60)91397-4

Cited by 32 publications

(11 citation statements)

References 9 publications

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“…8,9 Some of the earliest evidences for involvement of acetyl-CoA thioester in polyketide pathways were demonstrated with cell-free extracts from fungi. 10–12 In fact, the 6-methylsalicyclic acid ( 12 ) synthase (6MSAS) from the fungus Penicillium patulum is the first PKS to be purified and characterized in vitro . 13 Thus, the fungal polyketides have been central to the early formation of polyketide research.…”

Section: Introductionmentioning

confidence: 99%

Navigating the Fungal Polyketide Chemical Space: From Genes to Molecules

2012

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The iterative type I polyketide synthases (IPKSs) are central to the biosynthesis of an enormously diverse array of natural products in fungi. These natural products, known as polyketides, exhibit a wide range of biological activities and include clinically important drugs as well as undesirable toxins. The PKSs synthesize these structurally diverse polyketides via a series of decarboxylative condensations of malonyl-CoA extender units and β-keto modifications in a highly programmed manner. Significant progress has been made over the past few years in understanding the biosynthetic mechanism and programming of fungal PKSs. The continuously expanding fungal genome sequence data have sparked genome-directed discoveries of new fungal PKSs and associated products. The increasing number of fungal PKSs that have been linked to their products along with in-depth biochemical and structural characterizations of these large enzymes have remarkably improved our knowledge on the molecular basis for polyketide structural diversity in fungi. This perspective highlights the recent advances and examines how the newly expanded paradigm has contributed to our ability to link fungal PKS genes to chemical structures and vice versa. The knowledge will help us navigate through the logarithmically expanding seas of genomic information for polyketide compound discovery and provided opportunities to reprogram these megasynthases to generate new chemical entities.

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Section: Introductionmentioning

confidence: 99%

Navigating the Fungal Polyketide Chemical Space: From Genes to Molecules

2012

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“…If enzyme systems capable of cleaving aromatic rings (cf. Bassett & Tanenbaum, 1960) are relatively widespread among plants and micro-organisms, then it would seem reasonable to anticipate that a number of those organisms known to produce phenolic metabolites may also yield significant amounts of their ring-fission products.…”

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Studies in the biosynthesis of fungal metabolites. 4. Alternariol monomethyl ether and its relation to other phenolic metabolites of Alternaria tenuis

Thomas¹

1961

Biochemical Journal

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“…About 100 ,ug of ['4C]patulin with specific activity of 30 to 34 mCi/mmol was obtained, and the incorporation efficiency was 4.6 to 5.8%. This specific activity of [140]patulin is much higher than those reported in other investigations (2,6,11,15), and a product with such specific activity should be very useful in pharmacological, toxi-…”

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confidence: 69%

“…To study the metabolism and mode of action of patulin, it is essential to have labeled toxin with high specific activity. Various labeled precursors have been used in patulin biosynthetic studies (1, 2,6,11,15), but the specific activities of the patulin labeled in such studies have been rather low (0.05 to 3.05 mCi/mmol). In the present study, factors affecting the incorporation of [1-_4C]acetate into patulin in replacement culture are investigated.…”

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confidence: 99%

Production of [14C]patulin by Penicillium patulum

Nip¹,

Chu²

1977

Appl Environ Microbiol

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Factors affecting the production of [14C]patulin from [1-14C]acetate by replacement cultures of Penicillium patulum have been investigated. Incorporation of [1-14C]acetate into patulin reached a maximum with 6-to 8-day-old cultures incubated at 28°C for 8 h in a replacement medium containing 0.1 M glucose, inorganic salts, and undiluted [1-'4C]acetate. The specific activity of [14C]patulin obtained from this method was 34 mCi/mmol when 0.5 mCi of [1-14C]acetate was supplied to the replacement medium.

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Acetyl-coenzyme A in patulin biosynthesis

Cited by 32 publications

References 9 publications

Navigating the Fungal Polyketide Chemical Space: From Genes to Molecules

Navigating the Fungal Polyketide Chemical Space: From Genes to Molecules

Studies in the biosynthesis of fungal metabolites. 4. Alternariol monomethyl ether and its relation to other phenolic metabolites of Alternaria tenuis

Production of [14C]patulin by Penicillium patulum

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