Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple

Abstract

The effects of the plant signal molecule acetosyringone (AS) and the osmoprotectant betaine phosphate (BP) have been examined for their ability to increase the transformation efficiency of Agrobacterium tumefaciens (At), C58C1::pGV3850 harboring the binary vector pKIWI105. This binary plasmid encodes the β-glucuronidase (GUS) gene and was previously shown to be expressed exclusively in plant tissues. Bacteria were grown in one of two previously reported virulence induction media (MS20 and SIM) for 5h and GUS activity was measured fluorimetrically in individual 6 week old leaf discs as a quantitative measure of stable transformation events. Bacteria induced in MS20 supplemented with AS (0.1 mM) and BP (1 mM) showed a significant increase in GUS activity as compared to media containing AS or BP added singly or control media lacking the supplements. The effects of another osmoprotectant proline (1 mM) could replace the beneficial effects of betaine. No significant difference was observed among treatments with respect to the two induction media.

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

“…al.·Silvae Genetica (2009) [123][124][125][126][127][128] lence genes of A. tumefaciens (SHARMA et al, 2005) to increase transformation efficiency. Many reports showed that AS could remarkably improve the transformation efficiency in some plants, such as apple (JAMES et al, 1993), rice (HIEI et al, 1994), and maize (ISHIDA et al, 1996). However, a study from GODWIN et al (1991) suggested that adding AS could suppress virulence of some A. tumefaciens strains.…”

Applications of Biotechnology in Kiwifruit (Actinidia)

“…However, the degree to which these factors influence transformation efficiency varies with species and reports. Acetosyringone (AS), one of the phenolic compounds released by wounded plant tissue, and a signal molecule to ensure effective vir-induction and T-DNA transfer (Stachel et al, 1985;1986), has been widely used to increase transformation efficiency in various crops (James et al, 1993;H. Wu et al, 2003).…”

Citrus biotechnology: Achievements, limitations and future directions

“…It is generally used at a concentration of 100 μM in cocultivation medium in citrus . Its positive role as a stimulator in facilitating infection by Agrobacterium has been established in the transformation of many woody plants such as apple (James et al, 1993) and kiwifruit (Janssen and Gardner, 1993) and crop plants (Kumar and Rajam, 2005).…”