Acetone-butanol production from pentoses by Clostridium acetobutylicum

Ounine, K.; Petitdemange, H.; Raval, G.

doi:10.1007/bf00130841

Cited by 55 publications

(29 citation statements)

References 9 publications

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“…Although C. acetobutylicum can metabolize either Gluc or Xyl individually, Xyl metabolism is severely inhibited when Gluc is available (14)(15)(16)(17). The overexpression of an Escherichia coli transaldolase-encoding gene, talA, in C. acetobutylicum increased Xyl utilization when the pentose sugar was the sole source of car-bon (18); the transaldolase reaction mediates the formation of E4P and F6P, a glycolytic intermediate, by combining two PPP intermediates, GAP and sedoheptulose-7-phosphate (S7P) (Fig.…”

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confidence: 99%

Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum

Aristilde

Lewis

Park

et al. 2015

Appl Environ Microbiol

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Bacterial metabolism of polysaccharides from plant detritus into acids and solvents is an essential component of the terrestrial carbon cycle. Understanding the underlying metabolic pathways can also contribute to improved production of biofuels. Using a metabolomics approach involving liquid chromatography-mass spectrometry, we investigated the metabolism of mixtures of the cellulosic hexose sugar (glucose) and hemicellulosic pentose sugars (xylose and arabinose) in the anaerobic soil bacterium Clostridium acetobutylicum. Simultaneous feeding of stable isotope-labeled glucose and unlabeled xylose or arabinose revealed that, as expected, glucose was preferentially used as the carbon source. Assimilated pentose sugars accumulated in pentose phosphate pathway (PPP) intermediates with minimal flux into glycolysis. Simultaneous feeding of xylose and arabinose revealed an unexpected hierarchy among the pentose sugars, with arabinose utilized preferentially over xylose. The phosphoketolase pathway (PKP) provides an alternative route of pentose catabolism in C. acetobutylicum that directly converts xylulose-5-phosphate into acetyl-phosphate and glyceraldehyde-3-phosphate, bypassing most of the PPP. When feeding the mixture of pentose sugars, the labeling patterns of lower glycolytic intermediates indicated more flux through the PKP than through the PPP and upper glycolysis, and this was confirmed by quantitative flux modeling. Consistent with direct acetyl-phosphate production from the PKP, growth on the pentose mixture resulted in enhanced acetate excretion. Taken collectively, these findings reveal two hierarchies in clostridial pentose metabolism: xylose is subordinate to arabinose, and the PPP is used less than the PKP.

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mentioning

confidence: 99%

Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum

Aristilde

Lewis

Park

et al. 2015

Appl Environ Microbiol

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“…C. acetobutylicum ATCC 824 was obtained from the Russian National Collection of Industrial Microorganisms. This strain has proteolytic, saccharolytic and amylolytic ability (Croux, Canard & Goma, 1992;Bassam & Hans, 1990;Ounine, Petitdemange, Raval & Gay, 1983).…”

Section: Methodsmentioning

confidence: 99%

Biodetoxification of Lignocellulosic Hydrolysates by Specially Adapted Activated Sludge

Morozova¹

2017

European Proceedings of Social and Behavioural Sciences

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Realization of an inexpensive and effective detoxification method of lignocellulosic hydrolysates is a prerequisite for reducing the cost of biofuels production. We suggest a cost-effective waste-free method of hydrolysates purification from acetone-butanol inhibitors, which is based on the use of specially adapted activated sludge. We conducted the detoxification of an enzymatic hydrolysate of miscanthus cellulose and an acid hydrolysate of spruce by specially adapted activated sludge of the urban wastewater treatment plants. As a result of purification of the spruce hydrolysate, the concentration of 5-HMF has decreased from 3.06 g/L to 0.0012 g/L, furfural -from 1.3 g/L to 0.0009 g/L. The concentrations of 5-HMF have reduced from 1.03 g/L to 0.0013 g/L and the concentrations of furfural have decreased from 0.71 g/L to 0.0008 g/L. We showed the effectiveness of the method by fermentation of the medium based on treated or untreated hydrolysate by Clostridium acetobutylicum ATCC 824. The obtained results can be valuable not only for the production of biobutanol, but in the technologies for bioethanol production.

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“…Процесс брожения осуществлялся штаммом бакте-рий Clostridium acetobutylicum ATCC 824, по-лученным из ВКПМ. Данный штамм харак-теризуется способностью сбраживать как пентозы, так и гексозы (Ounine et al, 1983), а также обладает ярко выраженной амилоли-тической и протеолитиче-ской способностью .…”

Section: материалыunclassified

Evaluation of the possibility of using brewer’s spent grain for the fermentation of lignocellulosic hydrolysates to biobutanol

Morozova¹,

Semyonov²

2017

Principles of the Ecology

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Acetone-butanol production from pentoses by Clostridium acetobutylicum

Cited by 55 publications

References 9 publications

Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum

Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum

Biodetoxification of Lignocellulosic Hydrolysates by Specially Adapted Activated Sludge

Evaluation of the possibility of using brewer’s spent grain for the fermentation of lignocellulosic hydrolysates to biobutanol

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