Acetone–butanol–ethanol production from substandard and surplus dates by Egyptian native Clostridium strains

Isolation of nativeClostridiafor utilization of agroindustrial cherry waste into butanol

“…Although the literature does not show details regarding the methodology for the isolation of Clostridium, for soil samples, the previous treatment consists of drying the samples and sifting them. It suggests that this process leads to an increase in the density of the spores because elements such as land, among others, are eliminated by weight and size …”

“…Samples FY were sieved in a 300‐mm mesh (VWR, Pennsylvania, USA) and 0.3 g was mixed with 2 mL of filtered water. For other hand samples, FD were dried out at 37°C for a week, and 0.3 g was mixed with 2 mL of filtered water.…”

“…In recent decades, resources have been invested in researching and developing biotechnology to generate biofuels, such as biobutanol, which is presented as a feasible alternative when compared with traditional ethanol. It is similar to gasoline so it can be mixed with it, but in a higher ratio than ethanol .…”

Improvement of fungal lipids esterification process by bacterial lipase for biodiesel synthesis

“…The analysis of 16S rRNA genes was conducted as described by Abd-Alla et al [28], sequences of the 16S rRNA of strains were first analyzed using the advanced BLAST search program at the NCBI website: http://www.ncbi.nlm.nih.gov/BLAST/T in order to assess the degree of DNA similarity. The nucleotide sequences of the strains ASU 3, ASU 11, ASU 16 and ASU 32 were deposited in the GenBank nucleotide sequence database under accession numbers KP777551, KP777550, KP777549 and KP777552, respectively.…”

In situ hydrogen, acetone, butanol, ethanol and microdiesel production by Clostridium acetobutylicum ATCC 824 from oleaginous fungal biomass

“…In contrast to the traditional biofuel, ethanol, butanol has many advantages due to its nonhygroscopicity and low vapor pressure; it can be added to gasoline at any concentration and has a high potential for replacing petrochemical derived energy, these properties suggest that biobutanol could be a next generation biofuel [10e12]. Therefore, butanol production by fermenting acetoneebutanoleethanol (ABE)-producing clostridia has drawn much attention in recent years [13,14]. However, despite its many advantages, there are some impediments resulting from costs of substrate, butanol recovery from the fermentation broth, low productivity and difficulty in controlling culture metabolism [8,10].…”