Acetoacetyl-CoA reductase PhaB as an excellent anti-Prelog biocatalyst for the synthesis of chiral β-hydroxyl ester and the molecular basis of its catalytic performance

Wang, Ting; Yang, Ke; Tian, Qingfeng; Han, Ruiting; Zhang, Xuanshuo; Li, Aipeng; Zhang, Lianbing

doi:10.1016/j.mcat.2021.111854

Cited by 2 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCC 6803 SDR anti-Prelog promisc. 4RZI [170] KRED1-Pglu Pichia glucozyma SDR promiscuous 3KZV [171] ChemBioChem Remarkably, thermophilic dehydrogenases have been discovered in some psychrophilic organisms. For example, the structurally characterized Moraxella sp.…”

Section: Hvadh2mentioning

confidence: 99%

Stairway to Stereoisomers: Engineering Short‐ and Medium‐Chain Ketoreductases To Produce Chiral Alcohols

Shanbhag

2023

ChemBioChem

View full text Add to dashboard Cite

The short-and medium-chain dehydrogenase/reductase superfamilies are responsible for most chiral alcohol production in laboratories and industries. In nature, they participate in diverse roles such as detoxification, housekeeping, secondary metabolite production, and catalysis of several chemicals with commercial and environmental significance. As a result, they are used in industries to create biopolymers, active pharmaceutical intermediates (APIs), and are also used as components of modular enzymes like polyketide synthases for fabricating bioactive molecules. Consequently, random, semi-rational and rational engineering have helped transform these enzymes into product-oriented efficient catalysts. The rise of newer synthetic chemicals and their enantiopure counterparts has proved challenging, and engineering them has been the subject of numerous studies. However, they are frequently limited to the synthesis of a single chiral alcohol. The study attempts to defragment and describe hotspots of engineering short-and medium-chain dehydrogenases/reductases for the production of chiral synthons.

show abstract

Section: Hvadh2mentioning

confidence: 99%