Acetic Acid Separation from Anaerobically Treated Palm Oil Mill Effluent by Ion Exchange Resins for the Production of Polyhydroxyalkanoate byAlcaligenes eutrophus

Polyhydroxyalkanoate production from anaerobically treated palm oil mill effluent by new bacterial strain Comamonas sp. EB172

“…Organic acids concentration from the broth media was determined by high performance liquid chromatography (HPLC) (Shimadzu, LC-10 AS) (Hassan et al 1997). PHA content and composition of the lyophilized cell were determined using gas chromatography (Agilent, model 7890A) using an ID-BP1 capillary column, 30 9 0.32 9 0.25 lm (SGE).…”

“…The presence of unique microflora inside the lagoon, open and closed digesters coupled with rich cellulosic and lipid waste materials in POME led to new findings in recent years. Production of organic acids (Hassan et al 1997;Tabassum et al 2008), methane (CH 4 ) (Yacob et al 2006) and biohydrogen (Chong et al 2009) from POME were evidences of the presence of potential carbon and nitrogen sources al well as biological biodiversity in the POME sludge. Production of mixed organic acids from anaerobicallay treated palm oil mill effluent has introduced it as a shown renewable and cheaper carbon sources for PHAs production (Hassan et al 1997(Hassan et al , 2002Zakaria et al 2008).…”

Microbial Biopolimerization Production from Palm Oil Mill Effluent (POME)

“…With increasing concentrations of formic acid (for a pH maintained below 4), the PHA yield and content in Rhodobacter sphaeroides IFO 12203 dropped from 0.50 g/g and 67% to 0.21 g/g and 18%, respectively. Hassan et al (1997b) later found that the presence of sludge in the anaerobically treated POME inhibited PHA accumulation by R. sphaeroides IFO 12203. This was attributed to the PHA being produced in a POME without sludge as opposed to a treated POME with sludge.…”

“…A low concentration of ammonium would accelerate the PHA production in a synthetic waste with an organic acid profile, which was observed during POME treatment. However, Hassan et al (1997b) found that addition of ammonium and phosphate to anaerobically treated POME was required to maintain the cell activity and production of PHA since neither ammonium nor phosphate was present in the anaerobically treated POME. In total, the organic acid concentrations obtained from anaerobically treated POME were too low (Hassan et al, 1996;Salmiati et al, 2010) for it to be reused as raw material in the production of PHA on an industrial scale.…”

Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238

“…The production medium used for PHB production contained (in g/L): 2 K2HPO4, 6.7 KH2PO4, 1.0 (NH4)2SO4, 0.4 MgSO4. 7H2O, 0.1 CaCl2, 0.04 FeSO4, 2.0 Lactic acid and 1.0 mL of trace elements (Hassan et al, 1997a). The pH of the production medium was controlled at 6.8 to 7.0 using 2M H2SO4 and 2M NaOH.…”