Acetate Metabolism in Physiology, Cancer, and Beyond

Bose, Shree; Ramesh, Vijyendra; Locasale, Jason W.

doi:10.1016/j.tcb.2019.05.005

Cited by 151 publications

(107 citation statements)

References 57 publications

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“…Acetyl-CoA is a fundamental component of de novo fatty acid synthesis and cholesterol. As an acetyl donor for histone lysine acetylation, acetyl-CoA is also an essential molecule for signaling and epigenetics [1]. In this study, we revealed that nuclear FABP7 is closely associated with nuclear acetyl-CoA levels, as well as H3K27ac levels on the promoters of several genes.…”

Section: Discussionmentioning

confidence: 63%

“…7h and i, analysis was performed in the group of between GST-treated and FABP7wt treated or between FABPwttreated and FABP7mut-treated Shown are representative proteins interacted with FABP7wt and without FABP7mut, with their MASCOT protein scores, spectral counts, and number of sequence. False discovery rate (FDR) calculated by MASCOT decoy search was 3.10% 1 Protein score was determined by the MASCOT software 2 Number of uniquely assigned spectral count for the respective proteins is shown 3 Number of sequence match for the respective proteins is shown 4 Number of spectral count for the respective proteins in the mock purification is shown verified that ACLY interacts with FABP7 (Tables 2 and 3, and Fig. 7a).…”

Section: Discussionmentioning

confidence: 99%

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FABP7 Regulates Acetyl-CoA Metabolism Through the Interaction with ACLY in the Nucleus of Astrocytes

et al. 2020

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Fatty acid binding protein 7 (FABP7) is an intracellular fatty acid chaperon that is highly expressed in astrocytes, oligodendrocyte-precursor cells, and malignant glioma. Previously, we reported that FABP7 regulates the response to extracellular stimuli by controlling the expression of caveolin-1, an important component of lipid raft. Here, we explored the detailed mechanisms underlying FABP7 regulation of caveolin-1 expression using primary cultured FABP7-KO astrocytes as a model of loss of function and NIH-3T3 cells as a model of gain of function. We discovered that FABP7 interacts with ATP-citrate lyase (ACLY) and is important for acetyl-CoA metabolism in the nucleus. This interaction leads to epigenetic regulation of several genes, including caveolin-1. Our novel findings suggest that FABP7-ACLY modulation of nuclear acetyl-CoA has more influence on histone acetylation than cytoplasmic acetyl-CoA. The changes to histone structure may modify caveolae-related cell activity in astrocytes and tumors, including malignant glioma.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

FABP7 Regulates Acetyl-CoA Metabolism Through the Interaction with ACLY in the Nucleus of Astrocytes

et al. 2020

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“…Some of these differences may indeed be attributed to the presence of an ongoing malignant process. For instance, tumor cells have been shown to capture acetate as a carbon source to sustain growth [95]. In addition, the turnover and usage of choline metabolites like phosphocholine, branched chain amino acids such as valine, and energy-storing molecules like creatine are known to be markedly altered in neoplastic tissues [96,97].…”

Section: Discussionmentioning

confidence: 99%

Pilot Multi-Omic Analysis of Human Bile from Benign and Malignant Biliary Strictures: A Machine-Learning Approach

Urmán

Herranz

Uriarte

et al. 2020

Cancers

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Cholangiocarcinoma (CCA) and pancreatic adenocarcinoma (PDAC) may lead to the development of extrahepatic obstructive cholestasis. However, biliary stenoses can also be caused by benign conditions, and the identification of their etiology still remains a clinical challenge. We performed metabolomic and proteomic analyses of bile from patients with benign (n = 36) and malignant conditions, CCA (n = 36) or PDAC (n = 57), undergoing endoscopic retrograde cholangiopancreatography with the aim of characterizing bile composition in biliopancreatic disease and identifying biomarkers for the differential diagnosis of biliary strictures. Comprehensive analyses of lipids, bile acids and small molecules were carried out using mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (1H-NMR) in all patients. MS analysis of bile proteome was performed in five patients per group. We implemented artificial intelligence tools for the selection of biomarkers and algorithms with predictive capacity. Our machine-learning pipeline included the generation of synthetic data with properties of real data, the selection of potential biomarkers (metabolites or proteins) and their analysis with neural networks (NN). Selected biomarkers were then validated with real data. We identified panels of lipids (n = 10) and proteins (n = 5) that when analyzed with NN algorithms discriminated between patients with and without cancer with an unprecedented accuracy.

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“…Through the acetate pathway, compounds called polyketides are formed (by the condensation of acetic acid units, giving rise to poly-β-keto chains). In this class are fatty acids, polyacetylenes, prostaglandins, macrolide antibiotics, and various aromatic compounds, such as anthraquinones and tetracyclines [ 74 , 75 ].…”

Section: Secondary Metabolite Biosynthesismentioning

confidence: 99%

Plants as Sources of Anti-Inflammatory Agents

et al. 2020

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Plants represent the main source of molecules for the development of new drugs, which intensifies the interest of transnational industries in searching for substances obtained from plant sources, especially since the vast majority of species have not yet been studied chemically or biologically, particularly concerning anti-inflammatory action. Anti-inflammatory drugs can interfere in the pathophysiological process of inflammation, to minimize tissue damage and provide greater comfort to the patient. Therefore, it is important to note that due to the existence of a large number of species available for research, the successful development of new naturally occurring anti-inflammatory drugs depends mainly on a multidisciplinary effort to find new molecules. Although many review articles have been published in this regard, the majority presented the subject from a limited regional perspective. Thus, the current article presents highlights from the published literature on plants as sources of anti-inflammatory agents.

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Acetate Metabolism in Physiology, Cancer, and Beyond

Cited by 151 publications

References 57 publications

FABP7 Regulates Acetyl-CoA Metabolism Through the Interaction with ACLY in the Nucleus of Astrocytes

FABP7 Regulates Acetyl-CoA Metabolism Through the Interaction with ACLY in the Nucleus of Astrocytes

Pilot Multi-Omic Analysis of Human Bile from Benign and Malignant Biliary Strictures: A Machine-Learning Approach

Plants as Sources of Anti-Inflammatory Agents

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