Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance

Schneider, Karl H.; Enayati, Marjan; Grasl, Christian; Walter, Ingrid; Budinský, Luboš; Zebic, Gabriel; Kaun, Christoph; Wagner, Anja; Kratochwill, Klaus; Redl, Heinz; Teuschl, Andreas; Podesser, Bruno K.; Bergmeister, Helga

doi:10.1016/j.biomaterials.2018.05.045

Cited by 53 publications

(47 citation statements)

References 63 publications

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“…Besides, dUECM showed the best cell filtration ( Figure 5), scaffold recellularization/differentiation, and the formation of characteristic endometrial secretory glands, indicating the tissue regeneration promotion effect of the decellularized matrix compositions. It is pointed out that ECM preservation is critical for the function of acellular matrix to promote cell proliferation [34]. In our study, the major ECM compositions, including collagen IV, laminin, and fibrin, were well retained, which was also beneficial for cell seeding and expanding after xenotransplantation.…”

Section: Discussionsupporting

confidence: 55%

“…Many crosslinking methods have been exploited, but a suitable crosslinking strategy with balanced mechanical strength and bioactivity is still rare. The degraded extracellular matrix could promote the cell proliferation by providing the essential nutritional ingredients [34], but it is also crucial to provide extended mechanical support at the defected/ excised area for cell seeding, proliferation and differentiation within sufficient time to regenerate defect tissue. Here, we prepared PC-and GP-crosslinking dUECM at low, middle, and high crosslinking degree each and evaluated how the different crosslinking degrees affect the physicochemical properties of dUECM and the following in vivo performance.…”

Section: Discussionmentioning

confidence: 99%

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Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Yao

Zheng

Lin

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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2020) Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction, Artificial Cells, Nanomedicine, and Biotechnology, 48:1, 218-229, ABSTRACTDecellularized extracellular matrix (dECM) has been considered as a promising scaffold in xenotransplantation, yet natural tissue dECM is often mechanically weak and rapidly degraded, compromising the outcomes. How to restore the mechanical strength and optimise the in vivo degradation, but maintain the microstructure and maximumly suppress the immune rejection, remains challenging. For this aim, we prepared and characterised various crosslinked decellularized rabbit uterus matrix (dUECM) and evaluated in vivo performance after uterus xenotransplantation from rabbit to rat. Naturally derived genipin (GP) and procyanidins (PC) were chosen to crosslink the dUECM, producing significant mechanical enhanced crosslinked-dUECM along with prolonged enzymatic degradation rate. Xenogeneic subcutaneous graft studies revealed that PC-and GP-crosslinked dUECM experienced significant cell infiltration and caused low immune reactions, indicating the desired biocompatibility. In vivo transplantation of GP-and PC-crosslinked dUECM to a uterus circular excised rat yielded excellent recellularization ability and promoted uterus regeneration after 90 days. While the reconstruction efficacy of crosslinked dUECM is highly depended on the crosslinking degree, crosslinking condition must be carefully evaluated to balance the role of crosslinked dECM in mechanical and biological support for tissue regeneration promotion. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Yao

Zheng

Lin

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Some protocols report trypsin and hypo/hypertonic solutions used together with these detergents [27,50,56], while the use of enzymatic DNA and RNA removal as one of the final steps is also recommended [27,56]. All of these chemical methods report quite good results, both in vitro and in vivo, implying the use of detergents as a valuable approach to vascular decellularization, while improvements related to avoiding immunogenicity and cytotoxicity are required [121].…”

Section: Vascular Tissuementioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

152

129

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The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.

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“…Differing from cellular materials, immunogenicity of ECM components is generally conservative among species. Therefore, it is well tolerant when used as allografts (Chen et al, 2019a) or xenografts (Schneider et al, 2018). Decellularized IVD scaffolds significantly promoted MSC viability and increased Collagen type II, Collagen type II/type I, AGN, Sox-9, GPC3 expression in vitro, indicating a NP-like phenotype differentiation, and alleviated aggravation of disc height decline and microstructure disruption on rabbit model .…”

Section: Natural Biomaterialsmentioning

confidence: 99%

Biomaterials-Induced Stem Cells Specific Differentiation Into Intervertebral Disc Lineage Cells

Peng

Huang

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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Stem cell therapy, which promotes stem cells differentiation toward specialized cell types, increases the resident population and production of extracellular matrix, and can be used to achieve intervertebral disc (IVD) repair, has drawn great attention for the development of IVD-regenerating materials. Many materials that have been reported in IVD repair have the ability to promote stem cells differentiation. However, due to the limitations of mechanical properties, immunogenicity and uncontrollable deviations in the induction of stem cells differentiation, there are few materials that can currently be translated into clinical applications. In addition to the favorable mechanical properties and biocompatibility of IVD materials, maintaining stem cells activity in the local niche and increasing the ability of stem cells to differentiate into nucleus pulposus (NP) and annulus fibrosus (AF) cells are the basis for promoting the application of IVDregenerating materials in clinical practice. The purpose of this review is to summarize IVD-regenerating materials that focus on stem cells strategies, analyze the properties of these materials that affect the differentiation of stem cells into IVD-like cells, and then present the limitations of currently used disc materials in the field of stem cell therapy and future research perspectives.

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Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance

Cited by 53 publications

References 63 publications

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Biomaterials-Induced Stem Cells Specific Differentiation Into Intervertebral Disc Lineage Cells

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