Acellular human glans extracellular matrix as a scaffold for tissue engineering: in vitro cell support and biocompatibility

Fm, Egydio; Freitas, Filho; Sayeg, Kleber; Laks, Marcus; As, Oliveira; Almeida, Fernando G.

doi:10.1590/s1677-5538.ibju.2014.0422

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…MSCs on collagen membranes have shown to induce accelerated wound healing [54]. MSCs on ECM-derived scaffolds can increase the longevity and functionality of wound healing, and MSCs on these ECM scaffolds maintain integrity and vitality for up to 14 days [55]. Fibronectin and collagen I can induce MSC chemotaxis and haptotaxis [56].…”

Section: Discussionmentioning

confidence: 99%

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

et al. 2017

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Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties including an anti-inflammatory cytokine profile and the promotion of angiogenesis via expression of growth factors in pre-clinical models. MSCs encapsulated in poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly(ethylene glycol) (Gel-PEG-Cys) crosslinked hydrogels have led to controlled cellular presentation at wound sites with favorable wound healing outcomes. However, the therapeutic potential of MSC-loaded hydrogels may be limited by non-specific protein adsorption on the delivery matrix that could facilitate the initial adhesion of microorganisms and subsequent virulent biofilm formation. Antimicrobials loaded concurrently in the hydrogels with MSCs could reduce microbial bioburden and promote healing, but the antimicrobial effect on the MSC wound healing capacity and the antibacterial efficacy of the hydrogels is unknown. We demonstrate that minocycline specifically induces a favorable change in MSC migration capacity, proliferation, gene expression, extracellular matrix (ECM) attachment, and adhesion molecule and growth factor release with subsequent increased angiogenesis. We then demonstrate that hydrogels loaded with MSCs, minocycline, vancomycin, and linezolid can significantly decrease bacterial bioburden. Our study suggests that minocycline can serve as a dual mechanism for the regenerative capacity of MSCs and the reduction of bioburden in triple antimicrobial-loaded hydrogels.

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Section: Discussionmentioning

confidence: 99%

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

et al. 2017

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“…Studies have reported that Collagens are responsible for for maintaining the ECM structure. [26] Although it is widely considered decellularization with SDS is related to ECM ultrastructure disruption [27,28] but our SEM results demonstrated that the 3D ECM structures were preserved thus indicating that 1% SDS was safe to DTG scaffolds. Besides, LN and FN are involved in cell adhesion.…”

Section: Discussionmentioning

confidence: 64%

Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering

Weng

Xie

et al. 2020

Preprint

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Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM). Herein, we prepared the 3D thyroid scaffold from the decellularization rabbit thyroid. Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression. Therefore, these findings revealed that the adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.

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“…Histologically stratified epithelial layer and organized muscle fibre bundles were evident after 6 months. Egydio et al () investigated the biocompatibility, in vitro cell support, and biomechanical properties of decellularized human glans. Immunohistochemical staining showed maintenance of the architecture as well as the extracellular matrix components.…”

Section: Resultsmentioning

confidence: 99%

“…A limitation of this review is the low number of studies found on cell‐seeded TE of corporal tissue. Furthermore, included studies, apart from Egydio et al (), were all animal studies. Techniques are not yet applicable on human patients, but knowledge obtained from animal studies can assist in exploring the technological and biological possibilities in human TE of corporal bodies.…”

Section: Discussionmentioning

confidence: 99%

A systematic review on cell‐seeded tissue engineering of penile corpora

Vocht

Kemp

Iljas

et al. 2017

J Tissue Eng Regen Med

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Tissue engineering of corporal tissue is a new development in otherwise untreatable erectile dysfunction and in urethral reconstructions to treat hypospadias or severe urethral stricture disease. Multiple complications can arise with the current treatments, whereas engineered tissue, if well vascularized and existing of autologous cells, may lead to better results. The aim of this review was to provide an overview of literature on cell-seeded-based tissue engineering of corporal penile tissue. A literature search was performed following the PRISMA guidelines. Papers describing cell-seeded tissue engineering of corporal tissue were included. Studies using different techniques, such as intracavernous injection were excluded. Fifteen articles were included in the review. Twelve of these studies described engineering of the corpus cavernosum in animal models. Two articles were found on engineering of animal corpus spongiosum and one article on engineering of the human glans. Both synthetic scaffolds and biological scaffolds were used. The advantage of a biological, acellular scaffold was that the native, complex architecture of corporal tissue was maintained. Most studies used endothelial and smooth muscle cells from corporal origin, but stem cells were also investigated. Furthermore, dynamic culturing achieved an improved cell content and functionality. This review has summarized the developments in tissue engineering of corpus cavernosum and spongiosum tissue. Functional tissue has been developed in animal studies with the use of seeded cells on scaffolds. This knowledge will form a basis for the development of tissue engineering of corporal tissue for clinical applications.

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Acellular human glans extracellular matrix as a scaffold for tissue engineering: in vitro cell support and biocompatibility

Cited by 13 publications

References 24 publications

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering

A systematic review on cell‐seeded tissue engineering of penile corpora

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