Acellular dermal graft for repair of abdominal wall defects in rabbits

Abstract

Sixteen clinically healthy New Zealand white rabbits of either sex were divided into 2 equal groups (I and II) of 8 animals each. Under thiopental sodium (2.5 %) anaesthesia a 2 × 3 cm full-thickness abdominal wall defect in the mid-ventral abdominal wall was created and repaired with an acellular dermal graft (ADG) in all the animals of group I (test group). In animals of group II (control group) a full-thickness linear midline abdominal muscular wall incision was made and repaired with a continuous suture pattern using 2-0 nylon

Tissue Engineering for Tissue and Organ Regeneration

“…It was indeed an immediate response initiated by surgical trauma when the abdominal well defects were created which perhaps correlated with the inflammatory phase of defects. A similar observations was reported by many researchers (Singh et al, 2008;Zuki et al, 2007;Gangwar et al, 2006;Gamba et al, 2002;Tung et al, 2002). Moreover, the inflammatory process is a normal response to surgical injury and the presence of implant as foreign objects.…”

“…However, there was not even a single skeletal muscle fiber formation in the implant of control groups. This finding seems consistence with previous reports of biological biomaterials mediated wound repair (Singh et al, 2008;Zuki et al, 2007;Gangwar et al, 2006;Gamba et al, 2002;Tung et al, 2002). In contrast to the above cases, in both the treatment groups (I and II), skeletal muscle tissue regeneration were clearly observed.…”

“…Dullness, depression and partial anorexia in the immediate postoperative period was attributed to surgical trauma and inflammation at the site of reconstruction. A similar result was reported by Gangwar et al (2006) using acellular dermal graft for repair of abdominal wall defects in rabbits. Neither the treatment groups nor its control groups in both types of scaffolds have showed any wound complication and infection as shown in figure 11, since, the complication rate after repair of large tissue defects strongly depends on applied reconstructive material.…”

“…The repair of large soft tissue defects, especially abdominal wall defects, is still a challenge for surgeons and continues to be a significant problem for patients (Gangwar et al, 2006;Lai et al, 2003). Free muscle transfer from local or distant sites is commonly employed for the surgical repair of muscle-tissue defects, but this practice is frequently associated with significant donor-site morbidity (Wei et al, 1995).…”

Repair of abdominal wall hernias using acellular dermal matrix in goats

“…However, biological biomaterials in their native form tend to be more immunogenic and hence are decellularised to minimise their immunogenicity (Gilbert et al 2006). Acellular dermal matrix (ADM) grafts have been used by several researchers for the reconstruction of abdominal wall defects in rabbits (Gangwar et al 2006;Singh et al 2008;Eberli et al 2010;Ngo et al 2011) and rats (Kaya et al 2006) with excellent results. Although results of the preclinical animal studies have been promising, uses of acellular dermal grafts for the reconstruction of abdominal hernias in clinical situations are limited.…”