ACE- inhibitory and radical scavenging activities of bioactive peptides obtained from camel milk casein hydrolysis with proteinase K

Abstract

The aim of this study was to evaluate the effects of enzymatic hydrolysis of camel whole casein on their antioxidant and angiotensin-converting enzyme (ACE)-inhibitory properties. Whole camel casein was hydrolyzed by proteinase K (PK), and the hydrolysates were fractionized by ultrafiltration membranes into three fractions. Semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC) was used to differentiate the mixture of peptides in the 3 kDa permeate fractions. A fraction (F4) with potentials of ACE-inhibitory activity (IC50 = 73 μg.mL −1 ) and radical scavenging activity (IC50 = 6.8 μg.mL −1 ) was selected for further purification and fractionation. The fraction F4C obtained from a second step purification of F4 showed strong ACE-inhibitory activity (IC 50 = 36 μg.mL −1 ) as well as radical scavenging activity (IC 50 = 3.3 μg.mL −1 ). The results of this study suggest that whole camel casein can be considered as a promising source for the production of peptides with potential of ACE-inhibitory and antioxidant activities.

Effects of dynamic ultra-high pressure homogenization on the structure and functional properties of casein

“…Therefore, it is necessary to improve the utilization rate of casein. Wang et al [6] applied the heat treatment to improve the properties of casein in yak milk; Huang et al [7] studied the impact of ultrasound on the gelling properties of casein; Rahimi et al [8] conducted enzymatic hydrolysis of casein in camel milk using protease K to obtain the bioactive peptides that scavenged free radicals. In recent years, researchers have used dynamic ultra-high pressure homogenization (UHPH) to improve the structural and functional properties of some proteins.…”

Dipeptidyl peptidase IV (DPP-IV) inhibitory properties of camel milk protein hydrolysates generated with trypsin

“…A wide range of bioactivities including antihypertensive, antioxidant, antimicrobial, anti-inflammatory and immunomodulatory properties have been reported in the literature for camel milk protein-derived peptides (for reviews, see: Hailu et al, 2016;Mati et al, 2016). More particularly, the antioxidant, antimicrobial and angiotensin converting enzyme (ACE) inhibitory activities of camel milk protein hydrolysates appear to have been studied (Jrad et al, 2014;Kumar, Chatli, Singh, Mehta, & Kumar, 2016;Moslehishad et al, 2013;Rahimi et al, 2016;Salami et al, 2010). In certain instances more potent bioactivities (antimicrobial, ACE inhibition and antioxidant activity) have been reported for camel compared to bovine milk hydrolysates and fermentates (Moslehishad et al, 2013;Salami et al, 2010).…”

Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases

“…Proteinase K was selected based on our previous work with camel milk casein, where we reported that the peptide fractions resulted from proteinase K digestion had the best antioxidant properties. [13]. Thus, proteinase K was used in the current study to evaluate its effect on walnut proteins.…”