ACE consensus meeting report: Culture systems

Bolton, Virginia N.; Cutting, Rachel; Clarke, Helen; Brison, Daniel R.

doi:10.3109/14647273.2014.944417

Cited by 20 publications

(11 citation statements)

References 136 publications

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“…Earlier efforts to culture human embryos to the blastocyst stage in less sophisticated media resulted in acceptable blastocyst formation rates but very low pregnancy rates [5][6][7][8], indicating that human embryos are capable of developing in diverse culture media, not all of which appear to generate viable embryos with a capacity to implant and result in a pregnancy. The adaptability of human embryos to varying culture conditions has prompted investigation and discussions regarding possible downstream long-term effects of single versus sequential culture on pregnancy outcome and offspring phenotype [43][44][45][46][47].…”

Section: Discussionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate. Included studies randomized either oocytes/zygotes or women. Eligible oocyte/zygote studies were analyzed to assess the risk difference (RD) and 95 % confidence intervals (CI) between the two media systems; eligible woman-based studies were analyzed to assess the risk ratio (RR) and 95 % CI for clinical pregnancy rate. Results No differences were observed between single and sequential media for either ongoing pregnancy per randomized woman (relative risk (RR) = 0.9, 95 % CI = 0.7 to 1.3, two studies including 246 women, I 2 = 0 %) or clinical pregnancy per randomized woman (RR = 1.0, 95 % CI = 0.7 to 1.4, one study including 100 women); or miscarriage per clinical pregnancy: RR = 1.3, 95 % CI = 0.4 to 4.3, two studies including 246 participants, I 2 = 0 %). Single media use was associated with an increase blastocyst formation per randomized oocyte/ zygote (relative distribution (RD) = +0.06, 95 % CI = +0.01 to Declaration of Authors' Roles Conception and design (CR, LR, WPM, IAS, and NR-F); search strategy (WPM, IAS); data extraction (WPM, PAN, IGRV, CON), analysis, and interpretation (all authors); writing the article (all authors). All authors had full access to all the data in the study and approved the final manuscript.Capsule In conclusion, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6 blastocyst stage. +0.12, ten studies including 7455 oocytes/zygotes, I 2 = 83 %) but not top/high blastocyst formation (RD = +0.05, 95 % CI = −0.01 to +0.11, five studies including 3879 oocytes/zygotes, I 2 = 93 %). The overall quality of the evidence was very low for all these four outcomes. Electronic supplementary materialConclusions Although using a single medium for extended culture has some practical advantages and blastocyst formation rates appear to be higher, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6. Future studies comparing these two media systems in well-designed trials should be performed.

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Section: Discussionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

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“…Based on 'the back to nature' strategy, growth factors have attracted much attention because of their intrinsic existence in female genital tracts [1,28]. Now, whether it is necessary to supplement a specific growth to culture medium in human IVF procedure is a hot spot issue.…”

Section: Discussionmentioning

confidence: 99%

Effects of granulocyte-macrophage colony-stimulating factor supplementation in culture medium on embryo quality and pregnancy outcome of women aged over 35 years

Zhou

Chu

Sha

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study is to explore whether a low concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF) supplementation in culture medium is beneficial to infertile women aged over 35 years. Conclusion Although no benefit of GM-CSF on embryo quality was observed, the addition of this factor significantly decreased the occurrence of chemical pregnancy of women aged over 35 years, indicating the role of GM-CSF in improving implantation competence of embryos derived from elderly infertile women.

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“…Assisted reproductive technology (ART) procedures such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) bypass certain natural selection barriers and require in vitro culture (IVC) of gametes and embryos. This involves their exposure to polystyrene substrates in large volumes of media where they remain static, with limited nutrients and in contact with end products of metabolism (Bolton, Cutting, Clarke, & Brison, ). Further, embryos are manipulated through pipetting and exposed to temperature and pH oscillations, which can induce stress responses (Xie, Wang, Puscheck, & Rappolee, ).…”

Section: Introductionmentioning

confidence: 99%

Embryo responses to stress induced by assisted reproductive technologies

Ramos‐Ibeas

Heras

Gómez-Redondo

et al. 2019

Molecular Reproduction Devel

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Assisted reproductive technology (ART) has led to the birth of millions of babies. In cattle, thousands of embryos are produced annually. However, since the introduction and widespread use of ART, negative effects on embryos and offspring are starting to emerge. Knowledge so far, mostly provided by animal models, indicates that suboptimal conditions during ART can affect embryo viability and quality, and may induce embryonic stress responses. These stress responses take the form of severe gene expression alterations or modifications in critical epigenetic marks established during early developmental stages that can persist after birth. Unfortunately, while developmental plasticity allows the embryo to survive these stressful conditions, such insult may lead to adult health problems and to long-term effects on offspring that could be transmitted to subsequent generations. In this review, we describe how in mice, livestock, and humans, besides affecting the development of the embryo itself, ART stressors may also have significant repercussions on offspring health and physiology. Finally, we argue the case that better control of stressors during ART will help improve embryo quality and offspring health. K E Y W O R D S assisted reproductive technologies, bovine, embryo, human, mouse, stress

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ACE consensus meeting report: Culture systems

Cited by 20 publications

References 136 publications

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Effects of granulocyte-macrophage colony-stimulating factor supplementation in culture medium on embryo quality and pregnancy outcome of women aged over 35 years

Embryo responses to stress induced by assisted reproductive technologies

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