Accurate quantification of sphingosine-1-phosphate in normal and Fabry disease plasma, cells and tissues by LC-MS/MS with 13 C-encoded natural S1P as internal standard

Mirzaian, Mina; Wisse, Patrick; Ferraz, Maria J.; Marques, André R. A.; Gabriel, Tanit L.; Roomen, Cindy P. A. A. van; Ottenhoff, Roelof; Eijk, Marco van; Codée, Jeroen D. C.; Marel, Gijsbert A. van der; Overkleeft, Herman S.; Aerts, Johannes M. F. G.

doi:10.1016/j.cca.2016.05.017

Cited by 11 publications

(14 citation statements)

References 48 publications

(86 reference statements)

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“…The measurement of sphingoid bases (sphingosine, sphinganine, and sphingosine-1-phosphate) can be combined with the measurement of LSLs (Mirzaian et al 2016(Mirzaian et al , 2017.…”

Section: Lysosphingolipidsmentioning

confidence: 99%

Contribution of tandem mass spectrometry to the diagnosis of lysosomal storage disorders

Piraud

Pettazzoni

Lavoie

et al. 2018

J of Inher Metab Disea

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Tandem mass spectrometry (MS/MS) is a highly sensitive and specific technique. Thanks to the development of triple quadrupole analyzers, it is becoming more widely used in laboratories working in the field of inborn errors of metabolism. We review here the state of the art of this technique applied to the diagnosis of lysosomal storage disorders (LSDs) and how MS/MS has changed the diagnostic rationale in recent years. This fine technology brings more sensitive, specific, and reliable methods than the previous biochemical ones for the analysis of urinary glycosaminoglycans, oligosaccharides, and sialic acid. In sphingolipidoses, the quantification of urinary sphingolipids (globotriaosylceramide, sulfatides) is possible. The measurement of new plasmatic biomarkers such as oxysterols, bile acids, and lysosphingolipids allows the screening of many sphingolipidoses and related disorders (Niemann-Pick type C), replacing tedious biochemical techniques. Applied to amniotic fluid, a more reliable prenatal diagnosis or screening of LSDs is now available for fetuses presenting with antenatal manifestations. Applied to enzyme measurements, it allows high throughput assays for the screening of large populations, even newborn screening. The advent of this new method can modify the diagnostic rationale behind LSDs.

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“…The measurement of sphingoid bases (sphingosine, sphinganine, and sphingosine-1-phosphate) can be combined with the measurement of LSLs (Mirzaian et al 2016(Mirzaian et al , 2017.…”

Section: Lysosphingolipidsmentioning

confidence: 99%

Contribution of tandem mass spectrometry to the diagnosis of lysosomal storage disorders

Piraud

Pettazzoni

Lavoie

et al. 2018

J of Inher Metab Disea

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“…Liquid chromatography‐tandem mass pectrometry (LC‐MS/MS) is now well accepted technology because of greater sensitivity, quantitative and specificity . So far, LC‐MS/MS methods have been developed only for the determination of the S1P content in plasma . The correlative research in serum has so far not been widely reported.…”

Section: Introductionmentioning

confidence: 99%

“…The correlative research in serum has so far not been widely reported. Nevertheless, accurate quantification of S1P levels in serum still poses many difficulties to modern LC‐MS/MS technology, mainly due to variable effects of a wide variety of biological matrices, as well as the lack of proper matrices free of analytes or samples with known concentrations of analytes …”

Section: Introductionmentioning

confidence: 99%

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

Tang

Chen

et al. 2020

Thoracic Cancer

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Background Sphingosine 1‐phosphate (S1P), a bioactive lipid, has been shown to mediate cancer processes. Therefore, accurate qualitative and quantitative determination is essential. The current assay method is still cumbersome to be of practical use worldwide and the aim of this study was therefore to develop a fast, accurate, precise and efficient LC‐MS/MS method for targeted analyses of S1P in serum samples. Methods Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) is an established method used for monitoring and analyzing S1P levels in serum. We determined the level of serum S1P in 256 patients with lung cancer and 36 healthy donors, and used Spearman';s rank correlation analysis to evaluate the difference in serum S1P levels between radiotherapy and nonradiotherapy patients. Results Standard curves were linear over ranges of 25–600 ng/mL for S1P with correlation coefficient (r2) greater than 0.9996. The lower limit of quantifications (LLOQs) was 25 ng/mL. The intra‐ and interbatch precisions and accuracy was less than 10% for S1P. The recoveries of the method were found to be 80%–98%. Serum S1P levels in healthy donors were different from those in patients (P < 0.001). Of 256 lung cancer patients, 124 (48.4%) received radiotherapy and were identified to have concomitant low serum S1P levels (222.13 ± 48.63), whereas 132 (51.6%) who had not received radiotherapy were identified to have high levels (315.16 ± 51.06). The serum S1P levels were therefore associated with radiotherapy (Spearman's Rho = −0.653, P < 0.001). Conclusions Our results indicated that this new LC‐MS/MS method is rapid, sensitive, specific and reliable for the quantification of S1P levels in serum samples. The level of S1P in serum samples of patients with lung cancer who received radiotherapy was significantly lower than that in patients who did not receive radiotherapy. Key points An improved method was established to quantify S1P levels in human serum by LC‐MS/MS, which enabled the change in serum S1P levels in lung cancer patients to be monitored, in combination with radiotherapy, and their clinical significance to be analyzed.

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“…Therefore, S1P, as an inflammatory cytokine, is a potential biomarker to treat AA rats. To quantify low‐abundance S1P in biological fluids (such as plasma and lymph fluid), several bioanalytical methods are generally used, such as liquid chromatography–tandem mass spectrometry (LC–MS/MS) and enzyme‐linked immunosorbent assay (ELISA), which are primarily based on LC–MS/MS using a stable‐labeled internal standard (IS) (such as [ 13 C 2 D 2 ] S1P) or S1P homologs (such as 13 C 5 C18‐S1P and commercial C17‐S1P) (Egom et al, ; Lan et al, ; Mirzaian et al, ; Chipeaux et al, ). However, these methods require chemical derivatization and relatively long experimental times, as well as complex sample collection and processing.…”

Section: Introductionmentioning

confidence: 99%

UHPLC–MS/MS analysis of sphingosine 1‐phosphate in joint cavity dialysate and hemodialysis solution of adjuvant arthritis rats: Application to geniposide pharmacodynamic study

Dai

Wang

et al. 2019

Biomedical Chromatography

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Geniposide (GE) is an iridoid glycoside compound with anti‐inflammatory effect. The potential of sphingosine 1‐phosphate (S1P) as a plasma marker in human diseases was suggested recently in the literature, which demonstrated that, in patients with inflammatory diseases, plasma S1P was elevated. It follows that the obstructive coronary artery disease can be predicted with serum S1P. Therefore, S1P can also be potentially used as a pharmacodynamic marker to study adjuvant arthritis (AA) rats. In the current study, a UHPLC–MS/MS method combined with the microdialysis sampling technique (using FTY720 phosphate as an internal standard) was adopted and validated to measure S1P levels in the hemodialysis fluid and joint cavity dialysates of AA rats after oral administration of GE. A S1P concentration–time curve in the dialysate was established in this study. It was demonstrated that GE exerted an anti‐inflammatory effect by reducing AA‐induced elevated S1P levels. It is showed that changes in S1P concentrations over time can be used to monitor the pharmacodynamic effects of GE in treating AA rats in pharmacodynamic studies.

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Accurate quantification of sphingosine-1-phosphate in normal and Fabry disease plasma, cells and tissues by LC-MS/MS with 13 C-encoded natural S1P as internal standard

Cited by 11 publications

References 48 publications

Contribution of tandem mass spectrometry to the diagnosis of lysosomal storage disorders

Contribution of tandem mass spectrometry to the diagnosis of lysosomal storage disorders

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

UHPLC–MS/MS analysis of sphingosine 1‐phosphate in joint cavity dialysate and hemodialysis solution of adjuvant arthritis rats: Application to geniposide pharmacodynamic study

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