Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS: focus on selenoprotein P

Busto, M. Estela del Castillo; Oster, Caroline; Cuello-Nuñez, Susana; Deitrich, Christian L.; Raab, Andrea; Konopka, Anna; Lehmann, Wolf D.; Goenaga‐Infante, Heidi; Fisicaro, Paola

doi:10.1039/c6ja00122j

Cited by 21 publications

(15 citation statements)

References 23 publications

(66 reference statements)

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“…A more sophisticated version of the system includes a switching valve and a circuit containing a column with an affinity for albumin, allowing for the specific recovery of SelAlb during the loading run for its subsequent quantification (Figure 3d) [84,[100][101][102][103]. It can be refined by the integration of a SEC column into the system (Figure 3e-f) [104][105][106][107].…”

Section: Liquid Chromatography-inductively Coupled Plasma-mass Spectrometry(icp-ms)mentioning

confidence: 99%

“…The closest proxy protein has been for the moment a full-length human recombinant SELENOP in which the original 10 SeCys residues (Sec) were replaced by 10 cysteines (Cys). This replacement was achieved by point mutations of nucleotide triplets coding Sec to triplets coding Cys in the coding sequence for selenoprotein present in the expression vector used as a template for E.coli protein synthesis [100]. Selenium was introduced in the polypetide chain during cell-free protein E.coli synthesis in the form of SeMet or 76 Se-Met for the preparation of the SEPP1 standard and an isotopically labeled spike for isotope dilution analysis [118,119].…”

mentioning

confidence: 99%

“…Selenium was introduced in the polypetide chain during cell-free protein E.coli synthesis in the form of SeMet or 76 Se-Met for the preparation of the SEPP1 standard and an isotopically labeled spike for isotope dilution analysis [118,119]. The standard and spike were purified by SDS PAGE [100]. However imperfect the assumption of the similarity of the behavior of the standard and the spike might be, the use of such an isotopically enriched spike allowed the quantification by standard addition and isotope dilution analysis by ICP-MS after the purification of SELENOP by affinity chromatography.…”

mentioning

confidence: 99%

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Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Lamarche

Ronga

Szpunar

et al. 2021

IJMS

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Selenoprotein P (SELENOP) is an emerging marker of the nutritional status of selenium and of various diseases, however, its chemical characteristics still need to be investigated and methods for its accurate quantitation improved. SELENOP is unique among selenoproteins, as it contains multiple genetically encoded SeCys residues, whereas all the other characterized selenoproteins contain just one. SELENOP occurs in the form of multiple isoforms, truncated species and post-translationally modified variants which are relatively poorly characterized. The accurate quantification of SELENOP is contingent on the availability of specific primary standards and reference methods. Before recombinant SELENOP becomes available to be used as a primary standard, careful investigation of the characteristics of the SELENOP measured by electrospray MS and strict control of the recoveries at the various steps of the analytical procedures are strongly recommended. This review critically discusses the state-of-the-art of analytical approaches to the characterization and quantification of SELENOP. While immunoassays remain the standard for the determination of human and animal health status, because of their speed and simplicity, mass spectrometry techniques offer many attractive and complementary features that are highlighted and critically evaluated.

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Section: Liquid Chromatography-inductively Coupled Plasma-mass Spectrometry(icp-ms)mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Lamarche

Ronga

Szpunar

et al. 2021

IJMS

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“…Clark's grebe tissues. Protein extracts from Clark's grebe liver tissue (n = 3) and muscle tissue (n = 3) and extraction blanks (n = 3) were screened for the co-elution of Hg and Se with selenoproteins by double affinity (AF) high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS3,24 ). All samples were measured in parallel in theS9 same analytical run.…”

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confidence: 99%

Demethylation of Methylmercury in Bird, Fish, and Earthworm

Manceau

Bourdineaud

Oliveira

et al. 2021

Environ. Sci. Technol.

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122

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Toxicity of methylmercury (MeHg) to wildlife and humans results from its binding to cysteine residues of proteins, forming MeHg-cysteinate (MeHgCys) complexes that hinder biological functions. MeHgCys complexes can be detoxified in vivo, yet how this occurs is unknown. We report that MeHgCys complexes are transformed into selenocysteinate (Hg(Sec)4) complexes in multiple animals from two phyla (a waterbird, freshwater fish, and earthworms) sampled in different geographical areas and contaminated by different Hg sources. In addition, high energy-resolution Xray absorption spectroscopy (HR-XAS) and chromatography-ICP mass spectrometry of the waterbird liver support the binding of Hg(Sec)4 to selenoprotein P and biomineralization of Hg(Sec)4 to chemically inert nanoparticulate mercury selenide (HgSe). The results provide a foundation for understanding mercury detoxification in higher organisms, and suggest that the identified MeHgCys to Hg(Sec)4 demethylation pathway is common in nature.All data supporting the findings of this study are available within the paper and have been deposited in the U.S. Geological Survey repository ScienceBase. 31 The deposit includes all HR-XANES spectra, the Hg L3-edge HR-EXAFS spectrum of the Clark's grebe liver, and the Cartesian coordinates of the Hg(selenoneine)4 complex and the Hg10(SeMe)20 cluster.

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“…However, the element-specific chromatogram will reveal the presence of any impurity peak(s) containing the target atom so a traceable value for the target molecule can be calculated. This approach has been used for compounds such as selenoproteins, 8 ferritin-bound iron 9 and carboplatin adducts. 10…”

Section: Traceability In the Laboratorymentioning

confidence: 99%

Traceable measurements by atomic spectrometry

Sargent¹

2021

Spectrosc. Europe

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Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS: focus on selenoprotein P

Abstract: Systematic approach for the accurate quantification of selenoproteins (SEPP1) in human plasma/serum by IDA-LC-ICP-MS.

Cited by 21 publications

References 23 publications

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Demethylation of Methylmercury in Bird, Fish, and Earthworm

Traceable measurements by atomic spectrometry

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