Accurate identification of Australian mosquitoes using protein profiling

Lawrence, Andrea L.; Batovska, Jana; Webb, Cameron E.; Lynch, Stacey E.; Blacket, Mark J.; Šlapeta, Jan; Parola, Philippe; Laroche, Maureen

doi:10.1017/s0031182018001658

Cited by 20 publications

(31 citation statements)

References 58 publications

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“…pipiens complex using multiplex PCR method in itself. There has been some research undertaken using DNA barcoding [47] and protein profiling [48] to distinguish the subspecies of the complex mentioned above. Since the adopted primers in this study only focused on three forward primers (ACEquin, ACEpall, and ACEpip) and one backward primer (B1246s), plus the limitation of sampling to some extent, Cx.…”

Section: Discussionmentioning

confidence: 99%

Breeding Site Characteristics and Associated Factors of Culex pipiens Complex in Lhasa, Tibet, P. R. China

Baimaciwang

Yue

et al. 2019

IJERPH

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Characterizing the breeding sites of Culex pipiens complex is of major importance for the control of West Nile disease and other related diseases. However, little information is available about the characteristics and associated factors of the breeding sites of the Cx. pipiens complex in Lhasa, a representative high-altitude region in Southwestern China. In this study, a cross-sectional study concerning the breeding site characteristics and associated factors of the Cx. pipiens complex was carried out in Lhasa, Tibet from 2013–2016. Chi-square analysis and binary logistic regression analysis were applied to identify the key factors associated with the presence of Cx. pipiens complex larvae. Using a standard dipping method, 184 water bodies were examined and Cx. pipiens complex larvae were observed in 36 (19.57%) of them. There were significant differences in the composition of Cx. pipiens complex larvae among the breeding site stability (χ2 = 19.08, p = 0.00) and presence or absence of predators (χ2 = 6.986, p = 0.008). Binary logistic regression analysis indicated that breeding site stability and presence or absence of predators were significantly associated with the presence of Cx. pipiens complex larvae in Chengguan District, Lhasa. Relatively permanent water bodies such as water bodies along river fringes, ponds and puddles, and water bodies with no predators should be paid more attention for future Cx. pipiens complex larvae abatement campaigns in Lhasa, China.

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Section: Discussionmentioning

confidence: 99%

Breeding Site Characteristics and Associated Factors of Culex pipiens Complex in Lhasa, Tibet, P. R. China

Baimaciwang

Yue

et al. 2019

IJERPH

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“…have also highlighted the need for sharing and have expressed interest in an international database (Raharimalala et al ., 2017). Many reference spectra are also available on request or directly online (Lawrence et al ., 2019), substantially facilitating database creation. The MALDI-TOF mass spectrometry approach may also appear to be limited by the cost of the device but when the MALDI-TOF MS device is bought for clinical microbiology purposes, it can also be used for medical entomology at no additional cost.…”

Section: Discussionmentioning

confidence: 99%

Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling

et al. 2020

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BackgroundThe accurate and rapid identification of mosquito blood meals is critical to study the interactions between vectors and vertebrate hosts and, subsequently, to develop vector control strategies. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has been shown to be a reliable and effective tool for identifying single blood meals from mosquitoes.MethodsIn this study, we developed MALDI-TOF MS profiling protocols to identify Anopheles gambiae Giles, Anopheles coluzzii and Aedes albopictus mosquitoes’ mixed blood meals and the last of successive blood meals. The mosquitoes were either successively artificially fed with distinct host bloods or engorged with mixed bloods from distinct vertebrate hosts, such as humans, sheep and dogs.ResultsBlind test analyses revealed a correct identification of mixed blood meals from mosquitoes using MALDI-TOF MS profiling. The 353 MS spectra from mixed blood meals were identified using log score values >1.8. All MS spectra (n = 244) obtained from mosquitoes' successive blood meals were reproducible and specific to the last blood meal, suggesting that the previous blood meals do not have an impact on the identification of the last one.ConclusionMALDI-TOF MS profiling approach appears to be an effective and robust technique to identify the last and mixed blood meals during medical entomological surveys.

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“…MSPs were created on the basis of an algorithm using peak position, intensity and frequency data. Between two and nine new reference spectra per species were added to the lice database in our laboratory [26].…”

Section: Creation Of a Reference Spectra Databasementioning

confidence: 99%

“…Recently, MALDI-TOF MS has been evaluated as an efficient tool for the identification of arthropods including ticks [2,5], mosquitoes [26,28,46], culicoides [6,35], fleas [30,48], triatomines [25], tsetse flies [15], and phlebotomines [9] in laboratory and field conditions.…”

Section: Introductionmentioning

confidence: 99%

Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals

Ouarti

Laroche

Righi³

et al. 2020

Parasite

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.

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Accurate identification of Australian mosquitoes using protein profiling

Cited by 20 publications

References 58 publications

Breeding Site Characteristics and Associated Factors of Culex pipiens Complex in Lhasa, Tibet, P. R. China

Breeding Site Characteristics and Associated Factors of Culex pipiens Complex in Lhasa, Tibet, P. R. China

Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling

Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals

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