Accurate early prediction of tumour response to PDT using optical coherence angiography

Sirotkina, Marina A.; Moiseev, Alexander A.; Matveev, Lev A.; Zaitsev, Vladimir Yu.; Elagin, Vadim; Kuznetsov, Sergey S.; Gelikonov, Grigory V.; Ksenofontov, S. Yu.; Zagaynova, Elena V.; Feldchtein, Felix I.; Gladkova, Natalia D.; Vitkin, I. Alex

doi:10.1038/s41598-019-43084-y

Cited by 31 publications

(32 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Multimodal OCT has also proven to be very useful for in vivo evaluation of fairly rapid and pronounced posttherapeutic changes in tumors. For example, one can mention the OCT-based angiographic observation of blood-circulation blockages in tumors and in peri-tumorous regions for accurate prediction of the outcome of vasculature-targeted photodynamic therapy (PDT) during the first 24 hours post-PDT [6][7][8] . However, unlike the fairly easily observed perturbation in the microcirculation of blood, the assessment of the histological tissue structure lacks precise, noninvasive methods.…”

Section: Introductionmentioning

confidence: 99%

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

et al. 2020

Preprint

View full text Add to dashboard Cite

We present a non-invasive method based on Optical Coherence Elastography (OCE) enabling the in vivo segmentation of morphological tissue constituents, in particular, monitoring of morphological alterations during both tumor development and its response to therapies. The method uses compressional OCE to reconstruct tissue stiffness map as the first step. Then the OCE-image is divided into regions, for which the Young's modulus (stiffness) falls in specific ranges corresponding to the morphological constituents to be discriminated. These stiffness ranges (characteristic "stiffness spectra") are initially determined by careful comparison of the "goldstandard" histological data and the OCE-based stiffness map for the corresponding tissue regions. After such precalibration, the results of morphological segmentation of OCE-images demonstrate a striking correlation with the histological results in terms of percentage of the segmented zones. To demonstrate high sensitivity of the OCEmethod and its excellent correlation with conventional histological segmentation we present results obtained in vivo on a murine model of breast cancer in comparative experimental study of the efficacy of two anti-tumor chemotherapeutic drugs with different mechanisms of action. The new technique allowed in vivo monitoring and quantitative segmentation of (i) viable, (ii) dystrophic, (iii) necrotic tumor cells and (iv) edema zones very similar to morphological segmentation of histological images. Numerous applications in other experimental/clinical areas requiring rapid, nearly real-time, quantitative assessment of tissue structure can be foreseen.

show abstract

Section: Introductionmentioning

confidence: 99%

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Using OCT, one can evaluate both the general structure of biological tissue (number and thickness of its layers), the state of the connective tissue, and microcirculation (blood flow and lymph flow) in real time. This approach has already proven itself in solving a number of tasks, e.g., visualizing the microcirculation in the oral mucosa [16] and monitoring the vascular response to treatment [17,18].…”

Section: Introductionmentioning

confidence: 99%

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Sirotkina¹,

Potapov²,

Vagapova³

et al. 2019

Sovrem Tehnol Med

View full text Add to dashboard Cite

The aim of the study was to test the method of optical coherence tomography (OCT) for its ability to obtain images of blood and lymphatic vessels of the vulva.Materials and Methods. The study was performed using a multimodal optical coherence tomography device developed in the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod). In this setup, 3D images of 3.4×3.4×1.25 mm are created within 26 s. OCT angiography and OCT lymphangiography are based on the speckle structure analysis. Visualization of blood and lymphatic vessels does not require any additional contrast agents.A histological study of vulvar biopsy samples from two locations was performed in 3 patients without vulvar pathology and in 5 patients with vulvar lichen sclerosus; the results were then compared with 3D OCT images obtained at the same locations.Results. Using the multimodal OCT, we found that normal mucous membrane of the vulva had a well-developed network of blood and lymphatic vessels; their diameters were increasing with depth. In the subepithelial zone, only blood capillary loops could be seen, whereas lymphatic capillaries became detectable starting from a depth of 170 μm (submucosa layer).In the case of vulvar lichen sclerosus, the density of the blood vessel network decreases dramatically and the capillary loops in the subepithelial zone disappear. Lymphatic vessels become detectable at a depth of 300 μm, and only few thin vessels can be detected at depths of 300 to 600 μm. The results suggest that the presentation of blood and lymphatic vessels depends on the state of the vulvar connective tissue. Specifically, the number of blood and lymphatic vessels is reduced in areas of hyalinosis and sclerosis of collagen fibers.Conclusion. For the first time, using multimodal OCT, an in vivo study of blood and lymphatic vessels of the vulvar mucosa was performed on normal and lichen sclerosus-affected vulva; the obtained OCT images were then compared with histological images of the same tissues. The OCT method allows one to diagnose a zone of hyalinosis and sclerosis of collagen fibers characterized by an abnormally low number of blood and lymphatic vessels.

show abstract

“…2(a-c) (in the absence of additive noise), but for randomly distributed 5 10 2  scatterers, the density of which is close to concentration of cells in biological tissues. In the simulation, the axially moved particles displaced by 2.25x10 -4 m between the consequent A-scans, which corresponds to the velocity 2.25 m/s for the acquisition rate of the OCT scanner described in [9,24] and applied for angiographic mapping in [25,26,27,28] using the high-pass filtering principle. Figure 3a shows the initial B-scan through the axis of the vertical flow and Fig.…”

Section: Examples Of Refocusing Of Simulated Oct Scans Including the mentioning

confidence: 99%

Computationally efficient model of OCT scan formation by focused beams and its usage to demonstrate a novel principle of OCT-angiography

Matveyev¹,

Matveev²,

Moiseev³

et al. 2020

Laser Phys. Lett.

View full text Add to dashboard Cite

A computationally highly efficient full-wave model of OCT-scan formation by focused beams in spectral-domain optical coherence tomography (OCT) is presented. Similarly to some previous models, it is based on summation of fields scattered by discrete sub-resolution scatterers and enables accounting for axial and lateral inhomogeneity of the illuminating Gaussian beam. The main feature ensuring high computational efficiency of the described model is that instead of numerical integration of the scattered signal over the receiving aperture we apply analytical description of both the illuminating-beam focusing and collection of the scattered signals over the receiving aperture. Elimination of numerical integration over the receiving aperture has increased the computation speed by a factor of ~3 10. This is of key importance for practical feasibility of simulations of 3D OCT data volumes for large amounts (~7 5 10 10 ) of scatterers corresponding to realistic densities of cells in biological tissues. We demonstrate the model possibilities by simulating digital refocusing of strongly focused OCT beams in the presence moving scatterers. A novel principle of contrast-agent-free visualization of scatterer flows with velocities typical of blood microcirculation is demonstrated.

show abstract

Accurate early prediction of tumour response to PDT using optical coherence angiography

Cited by 31 publications

References 32 publications

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Computationally efficient model of OCT scan formation by focused beams and its usage to demonstrate a novel principle of OCT-angiography

Contact Info

Product

Resources

About