Accurate Determination of the Diffusion Coefficient of Proteins by Fourier Analysis with Whole Column Imaging Detection

Zarabadi, Atefeh S.; Pawliszyn, Janusz

doi:10.1021/ac503069g

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…As shown by experimental results, the calculation in the time domain, especially based on peak height and width, is not as effective as the frequency domain [14]. An average concentration detector would visualize the moving boundary signal as a sigmoid peak, which is not easy to deal with in the FT approach, however, using the concentration gradient detection (Schlieren); the output signal would have a derivative form that looks like a Gaussian-wise shape.…”

Section: Determination Of Diffusion Coefficientmentioning

confidence: 99%

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Development of a multichannel microfluidic system with Schlieren imaging microscopy for online chip-based moving boundary electrophoresis

Zarabadi

Pawliszyn

Hajialamdari

2017

Journal of Chromatography A

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The concentration gradient detection method based on the Schlieren optics employed for electrophoresis analyses by extending the technology to a multi-channel system using a prototyped microfluidic chip (thinXXS Micro-technology, Germany). The results prove that coupling a chip-based microfluidic device with Schlieren detection is an appropriate approach to improve the electrophoretic separations. The effects of channel's geometry and dimension were investigated by conducting the experiments in channels with different cross sectional areas. Fast kinetic data acquisition of the charge-coupled device (CCD) camera facilitated recording of a time sequence of optical images, demonstrating the potential of the CCD camera as a powerful tool for studying dynamic processes such as diffusion. Diffusion coefficients of sample proteins were measured under static and dynamic conditions, where the static mode demonstrated more accurate results. Furthermore, the Fourier transformation was employed to improve the Schlieren images for quantitative analysis of the diffusion coefficient measurement.

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Section: Determination Of Diffusion Coefficientmentioning

confidence: 99%

“…The present study explores the data analysis of Schlieren images, by calculating the diffusion coefficient in the frequency domain. The Fourier transformation approach is the same as what has been employed for analysis of the diffusion data from capillary isoelectric focusing with whole column imaging detection [14].…”

Section: Introductionmentioning

confidence: 99%

Development of a multichannel microfluidic system with Schlieren imaging microscopy for online chip-based moving boundary electrophoresis

Zarabadi

Pawliszyn

Hajialamdari

2017

Journal of Chromatography A

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“…Monoclonal antibody isoforms from healthy vs ovary and lymphatic cancer donors had similar retention times but α-1-Acid glycoprotein peak position and peak area were utilized to quantify differences [242]. Qualitative glycoprotein detection in cIEF can be achieved via whole column cIEF imaging [232,255,256] or cIEF-SDS [257].…”

Section: Peak Capacity Comparisonmentioning

confidence: 99%

Surface Enabled Lab-on-a-Chip (Loc) Device for Protein Detection and Separation

Wang¹

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Applications of capillary electrophoresis for biopharmaceutical product characterization

2021

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Capillary electrophoresis (CE), after being introduced several decades ago, has carved out a niche for itself in the field of analytical characterization of biopharmaceutical products. It does not only offer fast separation, high resolution in miniaturized format, but equally importantly represents an orthogonal separation mechanism to high‐performance liquid chromatography. Therefore, it is not surprising that CE‐based methods can be found in all major pharmacopoeias and are recommended for the analysis of biopharmaceutical products during process development, characterization, quality control, and release testing. Different separation formats of CE, such as capillary gel electrophoresis, capillary isoelectric focusing, and capillary zone electrophoresis are widely used for size and charge heterogeneity characterization as well as purity and stability testing of therapeutic proteins. Hyphenation of CE with MS is emerging as a promising bioanalytical tool to assess the primary structure of therapeutic proteins along with any impurities. In this review, we confer the latest developments in capillary electrophoresis, used for the characterization of critical quality attributes of biopharmaceutical products covering the past 6 years (2015–2021). Monoclonal antibodies, due to their significant share in the market, have been given prioritized coverage.

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Accurate Determination of the Diffusion Coefficient of Proteins by Fourier Analysis with Whole Column Imaging Detection

Cited by 6 publications

References 30 publications

Development of a multichannel microfluidic system with Schlieren imaging microscopy for online chip-based moving boundary electrophoresis

Development of a multichannel microfluidic system with Schlieren imaging microscopy for online chip-based moving boundary electrophoresis

Surface Enabled Lab-on-a-Chip (Loc) Device for Protein Detection and Separation

Applications of capillary electrophoresis for biopharmaceutical product characterization

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