Accurate determination of small one-bond heteronuclear residual dipolar couplings by F1 coupled HSQC modified with a G-BIRD(r) module

A novel liquid crystalline system for partial alignment of polar organic molecules

“…For monitoring the temperature dependence of the liquid crystalline system, a 15 min equilibration period was interleaved between successive temperatures. The 1 H, 13 C couplings ( 1 T CH = 1 J CH + 1 D CH ) [22] for 1 were measured from HSQC spectra with no decoupling in the 13 C dimension using a new pulse scheme including a BIRD filter within the HSQC sequence [23] at the same instrument using a TBI probe. The measurements for 2 were performed on a Bruker DRX-600 instrument equipped with a 5 mm TBI probe.…”

Visualization of enantiomers and determination of homo- and hetero-nuclear residual dipolar and scalar couplings: The natural abundant 13C edited J/D-resolved NMR techniques

“…The F 1 -coupled HSQC is another alternative wherein the advantage of homonuclear decoupling has been derived by the insertion of G-BIRD(r) module in the middle of F 1 dimension. This demands a longer experimental time as many t 1 -increments are required to achieve high resolution in the F 1 -dimension [31]. This disadvantage has been partially overcome by utilizing the J-resolved type of experiments, such as, J-resolved HMQC and J-resolved HSQC [32,33].…”

Measurement of one-bond 13Cα–1Hα residual dipolar coupling constants in proteins by selective manipulation of CαHα spins

“…To achieve a similar effect here, we use modified BIRD pulses [25]. Such pulses have been used previously to remove unwanted interaction from the indirectly detected dimensions of 2D experiments [32,33]. The modified BIRD pulse applied in the middle of the t 1 period eliminates evolution of proton-proton couplings that would otherwise accelerate signal decay.…”