Accurate detection of enzymatic degradation processes of gelatin–alginate microcapsule by 1H NMR spectroscopy: Probing biodegradation mechanism and kinetics

A multi-technique analysis of gelatin biodegradation on the surface of core–shell nanoparticles by Alteromonas macleodii extracellular proteases

“…For instance, the biodegradation of gelatin films by various bacteria and fungi has been studied by indirect impedance, weight loss and dehydrogenase activity, 57,58 without in-depth characterizing in detail the gelatin film at the nanoscale. More recently, the degradation process on a gelatin-alginate microcapsule by enzymes has been accurately characterized by 1 H NMR spectroscopy, 59 but the enzymes employed in this study were highly specific to the substrate and did not come from microorganisms. In another study, gelatin nanoparticles coated by red blood cell membranes were studied as an antibiotic delivery system able to release the drug triggered by bacterial exotoxins and using gelatinase-positive bacterial species.…”