Accurate and sensitive liquid chromatography/tandem mass spectrometry simultaneous assay of seven steroids in monkey brain

Bertin, Jonathan; Dury, Alain Y.; Ke, Yuyong; Ouellet, Johanne; Labrie, Fernand

doi:10.1016/j.steroids.2015.02.013

Cited by 8 publications

(9 citation statements)

References 65 publications

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“…When matrix effects cannot be minimized, e.g., including a cleanup step, an appropriate correction is necessary for an accurate quantification . In this work, the analyte ILIS was used as a surrogate, which allowed an efficient matrix correction and also a correction of potential errors associated with the sample treatment. , However, for some hormones, the analyte ILIS was not available. Although other labeled compounds eluting close to the analyte have been used as internal standards for quantification, , in this work we did not realize accurate quantification when the matrix effect was compensated using an ILIS analogue to the target hormone.…”

Section: Resultsmentioning

confidence: 99%

“…However, LC-MS/MS based methods present high sensitivity and specificity, reducing the sample preparation, and are very appropriate for (medium) high polar compounds. 5,9 Consequently, LC-MS/MS is widely used for steroids determination in mammalian samples, such as plasma, 11 milk, 12 urine 13,15 or brain. 15 Regarding fish samples/tissues, LC-MS/MS has been applied for the determination of steroids in plasma from several fish species, 2,[16][17][18][19][20] and to a lesser extent in other fish tissues.…”

Section: Introductionmentioning

confidence: 99%

“…5,9 Consequently, LC-MS/MS is widely used for steroid determination in mammalian samples, such as plasma, 11 milk, 12 urine, 13,14 or brain. 15 With regard to fish samples/tissues, LC-MS/MS has been applied for the determination of steroids in plasma from several fish species, 2,16−20 and to a lesser extent in other fish tissues. Wang et al 21 determined steroids in whole fish soft tissues with limits of detection (LOD) of ∼0.1 ng/g.…”

Section: Introductionmentioning

confidence: 99%

“…Analytical methodologies based on liquid chromatography (LC) or gas chromatography (GC) coupled to tandem mass spectrometry (MS/MS) provide improved specificity and allow the simultaneous multiclass determination of several hormones. , GC-MS/MS has been applied for the determination of steroids in blood, but the laborious sample preparation and low throughput prevent its wide use in laboratories. However, LC-MS/MS-based methods present high sensitivity and specificity, reducing the sample preparation time, and are very appropriate for (medium) highly polar compounds. , Consequently, LC-MS/MS is widely used for steroid determination in mammalian samples, such as plasma, milk, urine, , or brain . With regard to fish samples/tissues, LC-MS/MS has been applied for the determination of steroids in plasma from several fish species, ,− and to a lesser extent in other fish tissues.…”

Section: Introductionmentioning

confidence: 99%

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Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Serrano¹,

Beltrán²,

Piquer

et al. 2021

ACS Agric. Sci. Technol.

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Knowing the levels of steroids in fish is crucial to understand the reproductive processes during fish development and for reproductive success in aquaculture facilities. Although some of these compounds are present at very low concentrations, they play a relevant role in reproduction processes. Therefore, very sensitive and reliable analytical methodology is required for their accurate determination. In this work, ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) with triple quadrupole has been optimized for the identification and quantification of 13 steroids in plasma, muscle and ovaries of sole (Solea senegalensis) and, for the first time, in fish eggs and larvae. This is noteworthy because studying fish development in early stages could be the key to solving the reproductive problems observed in farmed specimens. Different strategies have been applied to solve analytical challenges such as matrix effects or the small amount of sample available. Compounds selected include progestagens (progesterone, 17α,20β-dihydroxypregnenone, 17α,20β,21trihydroxypregnenone), androgens (androstenedione, 11β-hydroxiandrostenedione, 11ketoandrostenedione, testosterone, 5α-dihydrotestosterone, 11-ketotestosterone), estrogens (estrone, estradiol), and corticosteroids (cortisol, 11-deoxycortisol). The methodology was validated in terms of accuracy (between 73-131%) and precision (relative standard deviations below 24%), at concentrations as low as 0.1 ng/mL for plasma, 0.1 ng/g for muscle and eggs, and 0.5 ng/g for ovaries and larvae. The application of the developed methodology to the analysis of samples from captive fish has allowed to identify and quantify steroids at sub-ng/mL or sub-ng/g.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Serrano¹,

Beltrán²,

Piquer

et al. 2021

ACS Agric. Sci. Technol.

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show abstract

“…Although GC–MS has a long history in steroid analysis, liquid chromatography-mass spectrometry (LC–MS) has become the current trend [ 7 , 8 ]. LC–MS, LC–MS/MS methodology have been proved to have higher selectivity, sensitivity, throughput and simpler sample pretreatment process than radioimmunoassay, GC–MS and GC–MS/MS [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative-Profiling Method of Serum Steroid Hormones by Hydroxylamine-Derivatization HPLC–MS

Liu

Chi

Fan

et al. 2019

Nat. Prod. Bioprospect.

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A sensitive and rapid high performance liquid chromatography–mass spectrometry (HPLC–MS) method was developed and validated for simultaneous quantification of ten steroid hormones, including estrogens, androgens, progesterones, and corticosteroids four classes of steroids. The following ten steroid hormones were analyzed: progesterone, 21-deoxycortisol, estrone, 4-androstenedione, testosterone, dihydro-testosterone, androstenone, dehydroepiandrosterone, corticosterone and cortisone. Stable deuterated isotopes were used as internal standards for quantification. Sample preparation with and without derivatization were performed after liquid–liquid extraction, and the corresponding results were compared according to sensitivity and selectivity. Hydroxylamine derivatization was found to improve the ionization efficiency of the analytes for electrospray ionization MS analysis. The gradient of mobile phase and experimental parameters for HPLC separation were optimized. The lower limits of quantification were in the range of 0.05–5 ng mL −1 with wide linear range for the ten steroid hormones. The intra-day precision < 11.1% and recovery of 84.5–120% with negligible matrix effect were achieved, where within the acceptance limits of the FDA guideline. Total HPLC–MS analysis time was 6 min. This method enables simultaneous quantification of steroids in human serum. It will be helpful for the serum steroid profiling in order to understand various endocrinology diseases. Graphical Abstract

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Determination of estrogens in human serum using a novel chemical derivatization–assisted liquid chromatography‐electrospray ionization‐tandem mass spectrometry method

Kang

Fan

Xiao

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale Assessing estrogen concentrations in biological systems can provide valuable information on physiological processes, which is crucial for the early diagnosis of many diseases. Because estrogens are present in the human body in low concentrations and in a wide dynamic range, analytical methods with high sensitivity and specificity are required for their determination in complex biological matrices. Methods To discover an appropriate derivatization reagent for estrogen mass spectrometry (MS) analysis, we compared five sulfonyl chloride derivatization reagents, namely 3‐methyl‐8‐quinolinesulfonyl chloride (MQSCl) and 8‐quinolinesulfonyl chloride (QSCl), 1‐methyl‐1H‐pyrazole‐4‐sulfonyl chloride, 1,2‐methyl‐imidazole‐5‐sulfonyl chloride, and dansyl chloride. By selecting the derivatization reagent with the best performance, we developed and validated a novel chemical derivatization–assisted‐liquid chromatography‐electrospray ionization‐tandem mass spectrometry (CD‐LC‐ESI‐MS/MS) method to simultaneously determine the concentrations of estrone, estradiol, and estriol (E1, E2, and E3) in human serum. Results It was found that among the five investigated reagents, MQSCl‐derivatized estrogens presented the highest sensitivity using LC‐ESI‐MS/MS. Based on this discovery, MQSCl was chosen to derivatize the analyzed estrogens to assist LC‐ESI‐MS/MS analysis. The limit of quantification of E1, E2, and E3 was measured as 2.7, 4.6, and 5.1 pg/mL, respectively. Inter‐ and intra‐day precision, expressed as the coefficient of variation, was shown to be lower than 13.2% for all concentrations. The mean recovery was 72.4% overall, with good reproducibility at low, medium, and high concentrations in the calibration range. Conclusions The developed method was successfully applied to the quantitative determination of estrogens in clinical human serum from pediatric and adult women, demonstrating the suitability of estrogen analysis in the biological matrix at low concentration (pg/mL).

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Accurate and sensitive liquid chromatography/tandem mass spectrometry simultaneous assay of seven steroids in monkey brain

Cited by 8 publications

References 65 publications

Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Quantitative-Profiling Method of Serum Steroid Hormones by Hydroxylamine-Derivatization HPLC–MS

Determination of estrogens in human serum using a novel chemical derivatization–assisted liquid chromatography‐electrospray ionization‐tandem mass spectrometry method

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