Accurate and reliable quantitation of short chain fatty acids from human feces by ultra high-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)

Chen, Li; Sun, Xiaowei; Khalsa, Amrik Singh; Bailey, Michael T.; Kelleher, Kelly J.; Spees, Colleen; Zhu, Jiangjiang

doi:10.1016/j.jpba.2021.114066

Cited by 22 publications

(10 citation statements)

References 48 publications

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“…Excellent chromatographic resolution and characteristic fragments of DMED/DEEA-SCFAs derivatives were also simultaneously achieved. These enhanced signals further improved the assay validity of the method with lower LOD, LOQ, and a better linear range as previously described ( 21 , 22 , 23 , 24 ).…”

Section: Resultssupporting

confidence: 65%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Resultssupporting

confidence: 65%

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Zhang

Yang

Wang

et al. 2021

Journal of Lipid Research

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“…This DNA was submitted for 16 S rRNA gene sequencing which will be reported in future studies. In addition, SCFA analysis were conducted on 40 samples, the results of which are reported previously [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…A Thermo Scientific Vanquish Flex UPLC coupled Q Exactive (QE) system was used to analyze derivatized SCFAs. According to the FDA guideline for bioanalytical method validation [28], the calibration curve, accuracy, precision, recovery and stability of targeted SCFAs were validated as described in our earlier study [29].…”

Section: Stool Short Chain Fatty Acid Detection and Validation Analysismentioning

confidence: 99%

Collection of biospecimens from parent-child dyads in a community garden-based nutrition intervention: protocol and feasibility

et al. 2022

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Background Non-invasive human biospecimens, including stool, urine, and hair, are important in understanding the relationship between diet and changes in human physiologic processes that affect chronic disease outcomes. However, biospecimen collection can be difficult when collecting samples for research studies that occur away from a centralized location. We describe the protocol and feasibility in collecting stool, urine, and hair biospecimens from parents and their children at a remote location as a part of a summer community garden-based intervention. Methods Stool, urine, and hair were collected as a part of the Summer Harvest Adventure (SHA) study, a randomized controlled, community garden-based intervention targeting children (ages 8–11 years) and their parents from low-resource neighborhoods. Biospecimens were collected from willing children and/or their parent/adult caregivers at baseline and post-intervention for evaluation of microbiome, metabolomics, and hair analyses among both intervention and control groups at a location distant from the academic laboratories conducting the analysis. The protocol used to assemble, deliver, collect, and process biospecimens are presented along with the frequencies with which specimens were successfully obtained. Results One hundred forty six participants (73 parent-child dyads) were part of the larger SHA study and thus eligible to provide a biospecimen. A total of 126 participants, 115 participants, and 127 participants consented to provide their hair, stool and urine samples, respectively. Of the participants that consented to provide a sample, 44 children (69.8%) and 38 parents (60.3%) provided at least one hair sample, 27 children (48.2%) and 37 parents (62.7%) provided at least one stool sample, and 36 children (57.1%) and 42 parents (65.6%) provided at least one urine sample. Sample collection at the offsite location, transport, and handling at the academic center were successful and all biospecimens were deemed adequate for analyses. DNA and metabolomics yield on a subset of stool samples obtained provided excellent results in terms of an abundance of species and metabolities, as would be predicted. Urine and hair analyses are underway. Conclusion Our work is one of the first to describe the feasibility of collecting human biospecimens, specifically stool, urine, and hair, from both parents and their children from low-resourced neighborhoods in a non-traditional garden research setting. Future work will report findings related to mechanisms between diet, microbiome, metabolites, and clinical outcomes.

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“…Different HPLC technologies have been used over years to quantify SCFAs [25,29,30], especially in studies where the intestinal microbiota was altered in association with various pathological conditions [31][32][33][34][35]. In our study, coupling tandem mass spectrometry to HPLC (HPLC-MS-MS) to quantify acetic, propionic, and butyric acids in microbial culture supernatants led to a very speci c and sensitive detection, which is properly required when low analyte concentrations are present, as in the case of SCFAs [36,37].…”

Section: Discussionmentioning

confidence: 99%

HPLC-MS-MS quantification of short-chain fatty acids secreted by probiotic strains

Calvigioni

Bertolini

Codini

et al. 2022

Preprint

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Short-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and butyric acids are often associated with systemic disorders, the administration of SCFA-producing microorganisms has been suggested as attractive approach to solve symptoms related to SCFAs deficiencies. In this research, nine probiotic strains (Bacillus clausii NR, OC, SIN, and T, Bacillus coagulans ATCC 7050, Bifidobacterium breve DSM 16604, Limosilactobacillus reuteri DSM 17938, Lacticaseibacillus rhamnosus ATCC 53103, and Saccharomyces boulardii CNCM I-745) commonly included in commercial formulations were tested for their ability to secrete SCFAs by using an improved and sensitive protocol in high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS-MS). All tested microorganisms were shown to secrete acetic acid, with only B. clausii and S. boulardii additionally able to produce propionic and butyric acids. Quantitative differences in the secretion of SCFAs were also evidenced. The application of HPLC-MS-MS may help in the analysis of SCFA production by probiotics, especially for their administration as targeted bacteriotherapy to improve SCFAs deficiencies.

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Accurate and reliable quantitation of short chain fatty acids from human feces by ultra high-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)

Cited by 22 publications

References 48 publications

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Integrated LC-MS metabolomics with dual derivatization for quantification of FFAs in fecal samples of hepatocellular carcinoma patients

Collection of biospecimens from parent-child dyads in a community garden-based nutrition intervention: protocol and feasibility

HPLC-MS-MS quantification of short-chain fatty acids secreted by probiotic strains

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