Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

Tissari, Päivi; Zumla, Alimuddin; Tarkka, Eveliina; Mero, Sointu; Savolainen, Laura; Vaara, Martti; Aittakorpi, Anne; Laakso, Sanna; Lindfors, Merja; Piiparinen, Heli; Mäki, Minna; Carder, C; Huggett, Jim F.; Gant, Vanya

doi:10.1016/s0140-6736(09)61569-5

Cited by 192 publications

(131 citation statements)

References 19 publications

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“…However, varying technologies have been described, clinical experience remains limited, and additional validation is needed before recommending these methods as an adjunct to or replacement for standard blood culture techniques [71][72][73]. In addition, susceptibility testing is likely to require isolation and direct testing of viable pathogens for the foreseeable future.…”

Section: Diagnosismentioning

confidence: 99%

Surviving Sepsis Campaign: International Guidelines for Management of Sepsis and Septic Shock: 2016

et al. 2017

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Objective: To provide an update to "Surviving Sepsis Campaign Guidelines for Management of Sepsis and Septic Shock: 2012". Design:A consensus committee of 55 international experts representing 25 international organizations was convened. Nominal groups were assembled at key international meetings (for those committee members attending the conference). A formal conflict-of-interest (COI) policy was developed at the onset of the process and enforced throughout. A stand-alone meeting was held for all panel members in December 2015. Teleconferences and electronic-based discussion among subgroups and among the entire committee served as an integral part of the development. Methods:The panel consisted of five sections: hemodynamics, infection, adjunctive therapies, metabolic, and ventilation. Population, intervention, comparison, and outcomes (PICO) questions were reviewed and updated as needed, and evidence profiles were generated. Each subgroup generated a list of questions, searched for best available evidence, and then followed the principles of the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system to assess the quality of evidence from high to very low, and to formulate recommendations as strong or weak, or best practice statement when applicable.

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Section: Diagnosismentioning

confidence: 99%

Surviving Sepsis Campaign: International Guidelines for Management of Sepsis and Septic Shock: 2016

et al. 2017

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“…However, blood culture typically requires 1-3 days to become positive for detection of bacteria. Previous reports suggest that microarray or quantitative polymerase chain reaction analyses may be a faster and sensitive method for clinical identification of bacterial infection (27). It is reported that procalcitonin, but not CRP and WBC counts, is associated with gram-negative bacteria (28).…”

Section: Discussionmentioning

confidence: 99%

Inflammatory molecules expression pattern for identifying pathogen species in febrile patient serum

Liu

Lin

et al. 2016

Experimental and Therapeutic Medicine

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Abstract.Inflammatory molecules, such as cytokines and chemokines, have been considered markers for bacterial or viral infection in serum of patients in numerous studies. The aim of the present study was to investigate whether we were able to identify the pathogen species through patterns of inflammatory molecules. A total of 132 patients with elevated body temperature (tympanic temperature, >38.3˚C) were recruited for this study. The concentrations of various inflammatory molecules in the patients' serum were evaluated using a cytometric bead array. Higher concentrations of interleukin (IL)-6 and IL-8 were detected in bacterial infection groups (patients with positive and negative blood cultures), as compared with the viral infection group. Viral infection (including influenza and dengue viral infections) was associated with higher concentrations of interferon-γ-inducible protein 10 (IP-10), as compared with the bacterial infection group. In addition, IL-8 levels in the gram-negative bacteria group were higher, as compared with the gram-positive bacteria group. However, IL-8 was insufficient for bacterial species identification. By contrast, dengue virus infection induced the highest serum level of IP-10 among all groups. In conclusion, detection of the patterns of inflammatory molecules may aid the subsequent management and treatment modalities in hospitals, although evaluation of these molecules alone may be insufficient for identifying the pathogen species.

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“…74 The new improved version of Prove-it™ (Prove-it™ StripArray version 2.0) provides more comprehensive answers with less effort. In particular, it detects ∼80 species of Gram-negative and Gram-positive bacteria, 13 species of fungi, as well as identifying mecA, an antibiotic resistance marker used to identify methicillin-resistant Staphylococcus aureus.…”

Section: Prove-it™ Sepsismentioning

confidence: 99%

“…The assay is easy to process, reducing the risk of human error and providing high throughput and reliability for the routine laboratory diagnosis. 74,76,77 The main strength of the test is that it offers essential information for the treatment of septic patients 18 hours faster than traditional methods. Several papers have been presented indicating a high sensitivity and specificity (95% and 99%, respectively), although a lower diagnostic sensitivity (62%) has also been observed, nevertheless in a previous study a significantly lower diagnostic sensitivity (62%) was observed.…”

Section: Prove-it™ Sepsismentioning

confidence: 99%

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In vitro diagnosis of sepsis: a review

Marcello

Tumolo²,

Donno³

et al. 2016

PLMI

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Sepsis, severe sepsis and septic shock, systemic inflammatory response, and other related manifestations represent a relevant medical problem with high morbidity and mortality, despite the improvements in diagnosis, treatment, and preventive measures over the last few decades. The limited knowledge of the pathophysiology in association with the lack of in vitro diagnostic methods for the certain and quick determination of the causative microbiological agents and their antibiotic resistance means the condition is still critical and of high impact in health care. The current gold standard method to detect the sepsis-causing pathogens, which is based on blood culture, is still insufficiently sensitive and slow. The new culture-independent molecular biology-based techniques can lead to the identification of a broad range of microorganisms and resistance markers within a few hours and with high sensitivity and specificity; nevertheless, limitations of, for example, the polymerase chain reaction-based methods still hamper their application in the clinical routine. This review summarizes the in vitro diagnostic methods and their approach in the clinical diagnosis of the bloodstream infections, and explores their advantages and disadvantages at the current state of the art. A quick analysis of the future prospective in multiplex technologies for microbiological diagnosis of sepsis is also provided.

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Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

Cited by 192 publications

References 19 publications

Surviving Sepsis Campaign: International Guidelines for Management of Sepsis and Septic Shock: 2016

Surviving Sepsis Campaign: International Guidelines for Management of Sepsis and Septic Shock: 2016

Inflammatory molecules expression pattern for identifying pathogen species in febrile patient serum

In vitro diagnosis of sepsis: a review

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