Accurate 3D morphological computational model reconstruction of suspended cells imaged through stratified media by the precise depth-varying point spread function method

Yang, Zewen; Zhang, Lu; Lv, Ning; Song, Chunli; Wang, Huijun; Luo, Liu; Li, Yuan; Zhao, Hong

doi:10.1364/oe.465309

Cited by 2 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different from traditional modeling methods which applied spheres and ellipsoids to represent the internal and external cellular structures, in this study clinical lymphocyte morphologies in suspension were detected layer by layer firstly by fluorescence confocal microscope (FCM). Their 3D morphologies of membranes and nuclei were reconstructed by our methods published before [32,33]. The membrane and nucleus morphologies of clinical lymphocytes then were obtained as the basic morphology models.…”

Section: Morphology Modelingmentioning

confidence: 99%

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

et al. 2022

Self Cite

View full text Add to dashboard Cite

Objective and Impact Statement. Distinguishing malignant lymphocytes from normal ones is vital in pathological examination. We proposed an inverse light scattering (ILS) method for label-free suspended lymphocytes with complex fine structures to identify their volumes for pathological state. Introduction. Light scattering as cell’s “fingerprint” provides valuable morphology information closely related to its biophysical states. However, the detail relationships between the morphology with complex fine structures and its scattering characters are not fully understood. Methods. To quantitatively inverse the volumes of membrane and nucleus as the main scatterers, clinical lymphocyte morphologies were modeled combining the Gaussian random sphere geometry algorithm by 750 reconstructed results after confocal scanning, which allowed the accurate simulation to solve ILS problem. For complex fine structures, the specificity for ILS study was firstly discussed (to our knowledge) considering the differences of not only surface roughness, posture, but also the ratio of nucleus to the cytoplasm and refractive index. Results. The volumes of membrane and nucleus were proved theoretically to have good linear relationship with the effective area and entropy of forward scattering images. Their specificity deviations were less than 3.5%. Then, our experimental results for microsphere and clinical leukocytes showed the Pearson product-moment correlation coefficients (PPMCC) of this linear relationship were up to 0.9830~0.9926. Conclusion. Our scattering inversion method could be effectively applied to identify suspended label-free lymphocytes without destructive sample pretreatments and complex experimental systems.

show abstract

Section: Morphology Modelingmentioning

confidence: 99%

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…It mitigates non-focal plane stray light by placing two conjugated pinholes in the illumination and detection light paths. Subsequently, it records fluorescence images from different layers through mechanical scanning in the depth direction to acquire comprehensive 3D structural information [ 1 , 2 ]. To minimize exposure to high-intensity lasers in LSCM, light-sheet microscopy employs a thin light sheet to illuminate the sample from the side.…”

Section: Introductionmentioning

confidence: 99%

Alternating projection combined with fast gradient projection (FGP-AP) method for intensity-only measurement optical diffraction tomography in LED array microscopy

Yang,

Zhang,

Liu

et al. 2024

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Optical diffraction tomography (ODT) is a powerful label-free measurement tool that can quantitatively image the three-dimensional (3D) refractive index (RI) distribution of samples. However, the inherent "missing cone problem," limited illumination angles, and dependence on intensity-only measurements in a simplified imaging setup can all lead to insufficient information mapping in the Fourier domain, affecting 3D reconstruction results. In this paper, we propose the alternating projection combined with the fast gradient projection (FGP-AP) method to compensate for the above problem, which effectively reconstructs the 3D RI distribution of samples using intensity-only images captured from LED array microscopy. The FGP-AP method employs the alternating projection (AP) algorithm for gradient descent and the fast gradient projection (FGP) algorithm for regularization constraints. This approach is equivalent to incorporating prior knowledge of sample non-negativity and smoothness into the 3D reconstruction process. Simulations demonstrate that the FGP-AP method improves reconstruction quality compared to the original AP method, particularly in the presence of noise. Experimental results, obtained from mouse kidney cells and label-free blood cells, further affirm the superior 3D imaging efficacy of the FGP-AP method.

show abstract

Accurate 3D morphological computational model reconstruction of suspended cells imaged through stratified media by the precise depth-varying point spread function method

Cited by 2 publications

References 33 publications

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

Scattering Inversion Study for Suspended Label-Free Lymphocytes with Complex Fine Structures

Alternating projection combined with fast gradient projection (FGP-AP) method for intensity-only measurement optical diffraction tomography in LED array microscopy

Contact Info

Product

Resources

About