Accuracy of the Local‐lesion Method for Measuring Virus Activity. I. Tobacco‐mosaic Virus

Spencer, Ernest L.; Price, W. C.

doi:10.1002/j.1537-2197.1943.tb14758.x

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…Uniform plants with 12-15 large leaves, were selected and the growing points removed prior to assay . Since lesion numbers on the test plant varied with the virus content of the inoculum, preliminary trials as suggested by SPENCER and PRICE (11) were made to obtain a workable virus concentration . Preparations both from infected tobacco and tomato leaf samples were tested at various dilutions ranging from crude juice to 1 :10,000 .…”

Section: Viral Assay Techniquementioning

confidence: 99%

Studies on the inheritance of resistance to tobacco virus in the tomato

1965

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Section: Viral Assay Techniquementioning

confidence: 99%

Studies on the inheritance of resistance to tobacco virus in the tomato

1965

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“…Xanthi nc for the quantification of ToBRFV in bioassay. It has been shown that there were no significant differences in susceptibility between leaves in different positions when plants were grown under reduced light intensity [72,73]. The influence of the various factors mentioned above could be kept at bay by applying appropriate experimental designs and using statistical analysis in the evaluation of the data obtained [73].…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that there were no significant differences in susceptibility between leaves in different positions when plants were grown under reduced light intensity [72,73]. The influence of the various factors mentioned above could be kept at bay by applying appropriate experimental designs and using statistical analysis in the evaluation of the data obtained [73]. When the number of inoculated half-leaf units from N. glutinosa plants exceeds 16, the accuracy of local lesion methods increases [73,74].…”

Section: Discussionmentioning

confidence: 99%

“…The influence of the various factors mentioned above could be kept at bay by applying appropriate experimental designs and using statistical analysis in the evaluation of the data obtained [73]. When the number of inoculated half-leaf units from N. glutinosa plants exceeds 16, the accuracy of local lesion methods increases [73,74]. Therefore, using at least 24 half-leaf units for each dilution makes the accuracy of the method adequate in a way that statistically, there was no significant difference between the leaf positions.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Models for Quantification of Tomato Brown Rugose Fruit Virus Based on a Bioassay Using a Local Lesion Host

Zarghani

Monavari

Ehlers

et al. 2022

Plants

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Considering the availability of serological and molecular biological methods, the bioassay has been paled into insignificance, although it is the only experimental method that can be used to demonstrate the infectivity of a virus. We compared goodness-of-fit and predictability power of five models for the quantification of tomato brown rugose fruit virus (ToBRFV) based on local lesion assays: the Kleczkowski model, Furumoto and Mickey models I and II, the Gokhale and Bald model (growth curve model), and the modified Poisson model. For this purpose, mechanical inoculations onto Nicotiana tabacum L. cv. Xanthi nc and N. glutionosa L. with defined virus concentrations were first performed with half-leaf randomization in a Latin square design. Subsequently, models were implemented using Python software and fitted to the number of local lesions. All models could fit to the data for quantifying ToBRFV based on local lesions, among which the modified Poisson model had the best prediction of virus concentration in spike samples based on local lesions, although data of individual indicator plants showed variations. More accurate modeling was obtained from the test plant N. glutinosa than from N. tabacum cv. Xanthi nc. The position of the half-leaves on the test plants had no significant effect on the number of local lesions.

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“…To assess and ensure both the precision and accuracy of estimated virus concentrations, the inclusion of a spike control—comprising an additional sample with a known virus concentration—is recommended. It has been demonstrated that the local lesion assay achieves a satisfactory level of accuracy when implemented with 16 replications [ 20 , 75 ]. For the establishment of models, the developers used 24 half-leaf units for each dilution, and the experiments were repeated three times.…”

Section: Application Of Infectivity Dilution Curve In Virucidal Effic...mentioning

confidence: 99%

Quantifying Plant Viruses: Evolution from Bioassay to Infectivity Dilution Curves along the Model of Tobamoviruses

Nourinejhad Zarghani,

Monavari,

Nourinejhad Zarghani

et al. 2024

Viruses

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This review describes the development of the bioassay as a means of quantifying plant viruses, with particular attention to tobamovirus. It delves into various models used to establish a correlation between virus particle concentration and the number of induced local lesions (the infectivity dilution curve), including the Poisson, Furumoto and Mickey, Kleczkowski, Growth curve, and modified Poisson models. The parameters of each model are described, and their application or performance in the context of the tobacco mosaic virus is explored. This overview highlights the enduring value of the infectivity dilution curve in tobamovirus quantification, providing valuable insights for researchers or practitioners of bioassays and theoreticians of modeling.

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Accuracy of the Local‐lesion Method for Measuring Virus Activity. I. Tobacco‐mosaic Virus

Cited by 13 publications

References 13 publications

Studies on the inheritance of resistance to tobacco virus in the tomato

Studies on the inheritance of resistance to tobacco virus in the tomato

Comparison of Models for Quantification of Tomato Brown Rugose Fruit Virus Based on a Bioassay Using a Local Lesion Host

Quantifying Plant Viruses: Evolution from Bioassay to Infectivity Dilution Curves along the Model of Tobamoviruses

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