Accuracy of the Local Lesion Method for Measuring Virus Activity. II. Tobacco-Necrosis, Alfalfa-Mosaic, and Tobacco-Ringspot Viruses

Price, W. C.; Spencer, Ernest L.

doi:10.2307/2437518

Cited by 12 publications

(7 citation statements)

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“…A steep doseresponse curve was found in wild-type plants, concordant with previous results for multipartite viruses (5,12). A rigorous analysis of the data with the classic infection model, which assumes that the three types of virus particles occur at the same frequency in the inoculum, was therefore highly congruent with these historical results.…”

Section: Discussionsupporting

confidence: 87%

“…Recent work on the IAH for Tobacco etch virus, a monopartite Potyvirus, confirmed various IAH model predictions (7,10,11). On the other hand, the relationship between the AMV dose and the number of local lesions has been reported to be steeper than IAH predictions (12). Therefore, we first set out to confirm that the data for the infection of wild-type and P2 plants do not support the IAH model, whereas data for the P12 plants were expected a priori to support the IAH model.…”

Section: Predictions Of a Simple Infection Modelmentioning

confidence: 88%

“…When k Ͼ 1, the infection presented above is referred to as the dependent-action (DA) model. Note that k can take values of less than 1, but this outcome is not expected here given previous results (5,12). Note that for fitting of the classic infection model, the combined dose (d) of all three particle types was used.…”

Section: ϫ2mentioning

confidence: 89%

“…Moreover, any experimental error (e.g., variation in virus dose or inoculum size) will also tend to make the dose-response curve smoother (20,21). Therefore, the observation of steep dose-response curves for plant multipartite viruses seems to be somewhat unlikely from the outset, and its apparent commonality is therefore striking (5,12).…”

Section: Predictions Of a Simple Infection Modelmentioning

confidence: 99%

“…Multipartite viruses were indeed discovered because of the effects of multipartition on the relationship between the dose and the local-lesion number. Price and Spencer (12) first reported that the relationship between the dose and the number of local lesions for Alfalfa mosaic virus (AMV), Tobacco necrosis virus, and Tobacco ringspot virus on locallesion hosts was steeper than predicted by the IAH model. It was quickly recognized that these steep dose-local-lesion relationships could be explained if there were complementation between different types of virus particles (13,14).…”

mentioning

confidence: 99%

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Effects of the Number of Genome Segments on Primary and Systemic Infections with a Multipartite Plant RNA Virus

Sánchez‐Navarro

Zwart

Elena

2013

J Virol

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bMultipartite plant viruses were discovered because of discrepancies between the observed dose response and predictions of the independent-action hypothesis (IAH) model. Theory suggests that the number of genome segments predicts the shape of the dose-response curve, but a rigorous test of this hypothesis has not been reported. Here, Alfalfa mosaic virus (AMV), a tripartite Alfamovirus, and transgenic Nicotiana tabacum plants expressing no (wild type), one (P2), or two (P12) viral genome segments were used to test whether the number of genome segments necessary for infection predicts the dose response. The dose-response curve of wild-type plants was steep and congruent with the predicted kinetics of a multipartite virus, confirming previous results. Moreover, for P12 plants, the data support the IAH model, showing that the expression of virus genome segments by the host plant can modulate the infection kinetics of a tripartite virus to those of a monopartite virus. However, the different types of virus particles occurred at different frequencies, with a ratio of 116:45:1 (RNA1 to RNA2 to RNA3), which will affect infection kinetics and required analysis with a more comprehensive infection model. This analysis showed that each type of virus particle has a different probability of invading the host plant, at both the primary-and systemic-infection levels. While the number of genome segments affects the dose response, taking into consideration differences in the infection kinetics of the three types of AMV particles results in a better understanding of the infection process.

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Section: Discussionsupporting

confidence: 87%