Accuracy of the CellaVision DM96 platform for reticulocyte counting

Abstract

Context:Many hematology laboratories have adopted semi-automated digital platforms for routine use and the evidence supporting their use is increasing.Aims:The CellaVision platforms are among the most thoroughly studied digital hematology platforms; we wished to determine the accuracy of CellaVision for reticulocyte counting.Design, Materials and Methods:We compared reticulocyte counts performed manually, using the Beckman Coulter LH750 automated analyzer and with the CellaVision DM96 platform. We analyzed the results for pair-wise correlation and bias, and precision.Statistical Analyses Used:Analyses were performed using Statistical Package for the Social Sciences software (SPSS), including Spearman's rho correlation coefficient, Friedman's two-way Analysis Of Variance (ANOVA) for comparison of distributions; bias was compared by way of mean and standard deviation.Results:The CellaVision reticulocyte counts correlated most strongly with those of the analyzer (often considered the benchmark test); the reticulocyte count distributions were noted not to be significantly different from each other across all three methods. The mean and standard deviation of bias were lowest in the comparison of CellaVision and LH750 counts.Conclusions:Our data provide additional support for the accuracy of digital hematology applications using the CellaVision DM96 platform.

Digital morphology analyzers in hematology: ICSH review and recommendations

“…Mahe et al found good correlation in the reticulocyte count between the CellaVision DM96, the Beckman Coulter LH750 automated analyzer, and the manual reticulocyte count …”

Toward five‐part differential of leukocytes based on electrical impedances of single cells and neural network

“…Microscopic examinations of blood smears have functioned as the golden approach of the five‐part differential of WBC, where leukocytes are treated with acidic eosin y for the staining of cytoplasm and basic methyl blue for the staining of nucleus, followed by morphology and color examinations and classifications [2–5]. Although this approach is well established, it suffers from two key limitations of low throughput and high labor intensive.…”

“…Since numbers and percentages of leukocyte subtypes are deeply correlated with the states of human health due to their key roles in immune systems, the five-part differential of leukocytes has functioned as a primary screening condition for a variety of diseases (e.g., inflammation, infection or leukemia) [1]. Microscopic examinations of blood smears have functioned as the golden approach of the five-part differential of WBC, where leukocytes are treated with acidic eosin y for the staining of cytoplasm and basic methyl blue for the staining of nucleus, followed by morphology and color examinations and classifications [2][3][4][5].…”