Accuracy of soluble human leukocyte antigen-G for predicting pregnancy among women undergoing infertility treatment: meta-analysis

Vercammen, M.; Verloes, A.; Velde, H. Van de; Haentjens, Patrick

doi:10.1093/humupd/dmn007

Cited by 59 publications

(52 citation statements)

References 40 publications

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“…Furthermore, HLA-G was claimed to be a predictor for pregnancy and a promising noninvasive tool for embryo selection (58). However, further studies combining single-embryo culture and single-embryo transfer with a highly sensitive HLA-G immunodetection technique in highly standardized conditions are needed to determine the true value of soluble HLA-G measurement in embryo culture medium (62).…”

Section: Discussionmentioning

confidence: 99%

HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos

Verloes¹,

Velde²,

LeMaoult

et al. 2011

The Journal of Immunology

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Human leukocyte Ag-G, a tolerogenic molecule that acts on cells of both innate and adaptive immunity, plays an important role in tumor progression, transplantation, placentation, as well as the protection of the allogeneic fetus from the maternal immune system. We investigated HLA-G mRNA and protein expression in human embryonic stem cells (hESC) derived from the inner cell mass (ICM) of blastocysts. hESC self-renew indefinitely in culture while maintaining pluripotency, providing an unlimited source of cells for therapy. HLA-G mRNA was present in early and late passage hESC, as assessed by real time RT-PCR. Protein expression was demonstrated by flow cytometry, immunocytochemistry, and ELISA on an hESC extract. Binding of HLA-G with its ILT2 receptor demonstrated the functional active status. To verify this finding in a physiologically relevant setting, HLA-G protein expression was investigated during preimplantation development. We demonstrated HLA-G protein expression in oocytes, cleavage stage embryos, and blastocysts, where we find it in trophectoderms but also in ICM cells. During blastocyst development, a downregulation of HLA-G in the ICM cells was present. This data might be important for cell therapy and transplantation because undifferentiated hESC can contaminate the transplant of differentiated stem cells and develop into malignant cancer cells.

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Section: Discussionmentioning

confidence: 99%

HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos

Verloes¹,

Velde²,

LeMaoult

et al. 2011

The Journal of Immunology

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“…In a recent meta-analysis it was concluded that the presence of sHLA-G in the supernatants of cultured embryos is moderately helpful to predict the ability to achieve pregnancy in women undergoing IVF treatment [34]. Additionally they suggested it to be more beneficial if the embryos are of good quality.…”

Section: Treatment Groups Percent Of Fetusses Lost (%)mentioning

confidence: 99%

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Kotzé

Hansen

Keskintepe

et al. 2010

J Assist Reprod Genet

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“…Pyruvate, lactate, glucose, and amino acids in the embryo culture medium have all been reported to correlate with embryo development and implantation potential (see [15,16] for review). Global assessment of the embryo culture media proteome [17] as well as specific proteins such as soluble human leukocyte antigen-G (sHLA-G) [18,19] have also been studied as predictors of embryo viability.…”

Section: Introductionmentioning

confidence: 99%

Receiver operating characteristic (ROC) analysis of day 5 morphology grading and metabolomic Viability Score on predicting implantation outcome

Seli

Bruce

Botros³

et al. 2010

J Assist Reprod Genet

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Purpose Assessment of embryo viability is a key component of in vitro fertilization (IVF) and currently relies largely on embryo morphology and cleavage rate. In this study, we used receiver operating characteristic (ROC) analysis to compare the Viability Score (generated by metabolomic profiling of spent embryo culture media using near infrared (NIR) spectroscopy) to morphologic grading for predicting pregnancy in women undergoing single embryo transfer (SET) on day 5. Methods A total of 198 spent embryo culture media samples were collected in four IVF centers located in the USA, Europe and Australia. First, 137 samples (training set) were analyzed by NIR to develop an algorithm that generates a Viability Score predictive of pregnancy for each sample. Next, 61 samples (validation set) were analyzed by observers blinded to embryo morphology and IVF outcome, using the Day 5 algorithm generated with the training set. Pregnancy was defined as fetal cardiac activity (FCA) at 12 weeks of gestation. Genet (2011) 28:137-144 DOI 10.1007 potential to be a better classifier for pregnancy outcome than morphology alone in women undergoing SET on day 5.

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Accuracy of soluble human leukocyte antigen-G for predicting pregnancy among women undergoing infertility treatment: meta-analysis

Abstract: Further research is needed with single-embryo culture, single-embryo transfer and highly sensitive detection techniques to determine the potential application of measuring sHLA-G in culture supernatant.

Cited by 59 publications

References 40 publications

HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos

HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Receiver operating characteristic (ROC) analysis of day 5 morphology grading and metabolomic Viability Score on predicting implantation outcome

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