Accuracy of microCT in the quantitative determination of the degree and distribution of mineralization in developing bone

Abstract

Accuracy of microCT with polychromatic radiation can be considered adequate for assessment of the degree of mineralization of developing bone. Therefore, this method provides a three-dimensional means by which to simultaneously investigate the bone structure as well as the degree of mineralization during development in a non-destructive manner and with high resolution.

Biomechanical effect of mineral heterogeneity in trabecular bone

“…An integration time of 1200 ms was applied to reduce noise substantially and to facilitate the discrimination between bone and background. The microCT was equipped with an aluminum filter and a correction algorithm that reduces the effects of beam hardening (Mulder et al, 2004). The scan time was 51.774.0 h for each specimen.…”

High-Resolution Imaging Techniques for Bone Quality Assessment

“…Various calibration procedures based on idealized phantoms or theoretical calculations have been established to derive relations between attenuation and bone mineral density [64,65,67,82,93,94]. Like clinical CT, the polychromatic X-ray source used in conventional mCT systems produces an X-ray beam with photons spanning a broad range of energies.…”

Effect of Food Consistency on the Degree of Mineralization in the Rat Mandible

“…It was averaged in each volume of interest. [16][17][18] It is important to recognize the difference between two mineralization parameters. The first, the degree of mineralization of bone, is the parameter that has been used in the present study.…”

“…The latter kept their original gray value that can be considered proportional to the local degree of mineralization. 16,20 The degree of mineralization was derived from the linear attenuation coefficient using a linear relation, which was calibrated with a phantom containing hydroxyapatite densities of 0, 100, 200, 400, and 800 mg/cm 3 . It was averaged in each volume of interest.…”

“…The scan resolution was 15 lm, the integration time was 1200 ms, and the beam intensity was 45 kV, which corresponds to an effective energy of approximately 24 keV. 16 The microCT system was equipped with an aluminum filter (0.5 mm) to remove low energy rays. The effect of beam hardening was further reduced by applying a correction algorithm that was developed by the manufacturer.…”