Accuracy of LAMP-TB Method for Diagnosing Tuberculosis in Haiti

Kaku, Takashi; Minamoto, Fujihiko; D'Meza, Richard; Morose, Willy; Boncy, Jacque; Bijou, Josette; Geffrard, Harry; Yoshida, Masahito; Mori, Toru

doi:10.7883/yoken.jjid.2015.519

Cited by 13 publications

(10 citation statements)

References 9 publications

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“…There are several published reports on the diagnostic potential of LAMP assays for TB diagnosis. The overall performance varied greatly across the studies (5, [14][15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis

et al. 2020

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A c c e p t e d M a n u s c r i p t SUMMARY: Diagnosis tuberculosis (TB) in endemic countries is challenging due to high caseloads and limited resources. A simple, cost-effective diagnostic test for rapid detection of Mycobacterium tuberculosis in clinical specimens is in need. In Thailand, we evaluated the performance of in-house loop-mediated isothermal amplification (LAMP) targeting M. tuberculosis 16s rRNA gene for TB diagnosis. A total of 252 sputum samples from pulmonary TB suspects were analyzed. The sensitivity of LAMP was 99.04% (103/104; 95% confidential interval [CI]: 94.76-99.98%) and 72.73% (16/22; 95% CI: 49.78-89.27) for smear-positive and smear-negative samples with TB-culture positivity, respectively. LAMP detected 20.69% (24/116) of TB-culture negative samples but those all were positive by conventional PCR. The sensitivity of LAMP was higher than that of sputum microscopy while the performance of LAMP was similar to PCR. None of the samples positive for nontuberculous mycobacteria by culture and PCR was positive by LAMP. Compared with TB culture, the positive predictive value (PPV), negative predictive value (NPV) and kappa coefficient of LAMP were 83.22%, 88.33% and 0.75 respectively. Based on the diagnostic performance, we proposed that this LAMP would be suitable as a potential diagnostic test for rapid TB diagnosis in resource-limited laboratory settings.

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“…There are several published reports on the diagnostic potential of LAMP assays for TB diagnosis. The overall performance varied greatly across the studies (5, [14][15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis

et al. 2020

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“…The LAMP technique has been adapted for use in the diagnosis of different infectious diseases with notoriously high burdens, including tuberculosis and malaria, for which high sensitivity, specificity, affordability, and applicability to the tropical field are desired [ 24 ]. Among the advantages of this kit compared to PCR are that it requires less investment, staff training, and operating time.…”

Section: Discussionmentioning

confidence: 99%

Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spots

Vincent

Komaki-Yasuda

Iwagami

et al. 2018

Malar J

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BackgroundMalaria is one of the most important parasitic infectious diseases for which almost half of the world’s population is at risk. Although several diagnostic methods are now available to detect the infection, more sensitive and applicable tests are still required in the field. The loop-mediated isothermal amplification (LAMP) method is a DNA amplification tool in which the DNA amplification can be achieved by incubation at a stable temperature. A malaria detection kit based on this methodology has already been commercialized and is being used in some countries. The kit includes two reaction tubes: one targeting the common Plasmodium genus (Pan tube) and the other specifically targeting Plasmodium falciparum (Pf tube). In parallel, a simple DNA extraction method, the procedure for ultra rapid extraction (PURE), which can produce a DNA solution suitable for the LAMP reaction without the use of a centrifuge, has also become available. In this study, the sensitivity of the combination of the PURE and LAMP methods (PURE–LAMP) was evaluated with archived dried clinical blood samples of imported malaria cases, including P. falciparum, Plasmodium vivax, Plasmodium ovale, and Plasmodium malariae.ResultsUsing a nested PCR as the reference, 117 samples including 46 P. falciparum, 7 P. vivax, 9 P. ovale, 4 P. malariae, and 51 negative cases were tested. The PURE–LAMP Pan correctly identified 64 of the 66 positives and the 51 negatives. Among the Pan-positive samples 45 P. falciparum were also detected with the PURE–LAMP Pf. The PURE–LAMP Pan and PURE–LAMP Pf had respective sensitivities of 96.96% (95% CI 89.47–99.63) and 97.82% (95% CI 88.47–99.94) and common specificity of 1.ConclusionThe PURE–LAMP system is accurate when used with dried blood spots and extendable to the field.Electronic supplementary materialThe online version of this article (10.1186/s12936-018-2527-7) contains supplementary material, which is available to authorized users.

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“…Several evaluation studies have been conducted in various countries. However, the performance indicators varied greatly across the studies [ 10 11 12 13 ]. Previous studies have shown that TB-LAMP has lower sensitivity than fluorescence microscopy (FM) and that the performance of TB-LAMP is insufficient in smear-negative samples [ 14 15 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis

Kim

Cho²,

Shin³

et al. 2018

Ann Lab Med

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BackgroundEarly detection of tuberculosis (TB) is challenging in resource-poor settings because of limited accessibility to molecular diagnostics. The aim of this study was to evaluate the performance of the loop-mediated isothermal amplification kit (TB-LAMP) for TB diagnosis compared with conventional and molecular tests.MethodsA total of 290 consecutive sputum samples were collected from May till September, 2015. All samples were processed using the N-Acetyl-L-cysteine (NALC) NaOH method and tested by smear microscopy, solid and liquid culture, real-time PCR, and TB-LAMP.ResultsThe sensitivity of TB-LAMP for smear-positive and smear-negative samples with culture positivity was 92.0% and 58.8%, respectively. TB-LAMP was positive in 14.9% of TB culture-negative samples; however, all those samples were also positive by real-time PCR. In addition, none of the samples positive for nontuberculous mycobacteria by culture were positive by TB-LAMP. The overall agreement between TB-LAMP and real-time PCR was good; however, the concordance rate was significantly lower for real-time PCR positive samples with Ct values of 30–35.ConclusionsTB-LAMP could replace smear microscopy and increase TB diagnostic capacity when Xpert MTB/RIF is not feasible because of poor infrastructure.

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Accuracy of LAMP-TB Method for Diagnosing Tuberculosis in Haiti

Cited by 13 publications

References 9 publications

Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis

Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis

Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spots

Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis

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