Accuracy of electromagnetic models to estimate cardiomyocyte membrane polarization

Milan, Hugo Fernando Maia; Bassani, Rosana A.; Santos, Luiz Eduardo Canton; Almeida, Antônio-Carlos G. de; Bassani, José Wilson Magalhães

doi:10.1007/s11517-019-02054-2

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…The results of real shaped cells as obtained from e.g. a z-stack using confocal microscopy could be also considered as important but as it was shown recently, the modeling of (primary) cardiomyocytes as prolate spheroids is a good approximation approximation of stationary state with decreased computational cost in comparison to modeling of the real 3D geometry 62 .…”

Section: Methodsmentioning

confidence: 99%

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Dermol–Černe

Napotnik

Reberšek

et al. 2020

Sci Rep

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In gene electrotransfer and cardiac ablation with irreversible electroporation, treated muscle cells are typically of elongated shape and their orientation may vary. Orientation of cells in electric field has been reported to affect electroporation, and hence electrodes placement and pulse parameters choice in treatments for achieving homogeneous effect in tissue is important. We investigated how cell orientation influences electroporation with respect to different pulse durations (ns to ms range), both experimentally and numerically. Experimentally detected electroporation (evaluated separately for cells parallel and perpendicular to electric field) via Ca2+ uptake in H9c2 and AC16 cardiomyocytes was numerically modeled using the asymptotic pore equation. Results showed that cell orientation affects electroporation extent: using short, nanosecond pulses, cells perpendicular to electric field are significantly more electroporated than parallel (up to 100-times more pores formed), and with long, millisecond pulses, cells parallel to electric field are more electroporated than perpendicular (up to 1000-times more pores formed). In the range of a few microseconds, cells of both orientations were electroporated to the same extent. Using pulses of a few microseconds lends itself as a new possible strategy in achieving homogeneous electroporation in tissue with elongated cells of different orientation (e.g. electroporation-based cardiac ablation).

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Section: Methodsmentioning

confidence: 99%

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Dermol–Černe

Napotnik

Reberšek

et al. 2020

Sci Rep

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show abstract

“…Artman et al, 2000;Bassani & Bassani, 2002), associated with the much smaller cytosolic volume. Based in previous data on NCX-mediated Ca 2+ fluxes and cell geometry (Bassani & Bassani, 2002;Milan et al, 2019; present results), it can be estimated that the amount of Na + that enters the neonate cell via NCX per twitch is 0.40% of the amount required to increase [Na + ] i by 10 mm, vs. 0.12% in adult myocytes. The virtual absence of the T-tubule network during the first post-natal week (Ziman et al, 2010) may also allow rapid access of the inflowing Na + to the bulk cytosol.…”

Section: Developmental Differences In Na + Handlingmentioning

confidence: 58%

Developmental differences in myocardial transmembrane Na⁺ transport: implications for excitability and Na⁺ handling

Oshiyama

Pereira

Cardoso

et al. 2022

The Journal of Physiology

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support-information-section).Natália Ferreira Oshiyama obtained her PhD degree in Electrical Engineering from the School of Electrical and Computer Engineering of the University of Campinas, in Brazil. She is currently a researcher at the Center for Biomedical Engineering of the University of Campinas. Her research interests are understanding mechanisms involved in generation of electrical activity of cardiomyocytes, in both physiological and pathophysiological conditions, and also evaluating developmental differences. Much of her research work focuses on electrophysiological and fluorescence experiments, and also mathematical modelling of biophysical processes.

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Evaluating the Mechanical Properties of Isolated Rat Cardiomyocytes Sarcolemma Using Atomic Force Microscopy

Almazloum,

Cotta,

Bassani

et al. 2023

IFMBE Proceedings

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Accuracy of electromagnetic models to estimate cardiomyocyte membrane polarization

Cited by 6 publications

References 38 publications

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Developmental differences in myocardial transmembrane Na⁺ transport: implications for excitability and Na⁺ handling

Evaluating the Mechanical Properties of Isolated Rat Cardiomyocytes Sarcolemma Using Atomic Force Microscopy

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Accuracy of electromagnetic models to estimate cardiomyocyte membrane polarization

Cited by 6 publications

References 38 publications

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Developmental differences in myocardial transmembrane Na+ transport: implications for excitability and Na+ handling

Evaluating the Mechanical Properties of Isolated Rat Cardiomyocytes Sarcolemma Using Atomic Force Microscopy

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Product

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Developmental differences in myocardial transmembrane Na⁺ transport: implications for excitability and Na⁺ handling