Police officers engage in movements such as foot pursuits, sudden directional changes, or prolonged sprinting while wearing occupational loads (including body armor, baton, radio, handcuffs, and sidearm). This study investigated the effects of incrementally heavier loads carried by police students on their physical performance. Police students (n 5 63; male n 5 39 male and 24 female students) performed an acceleration over a 10-m distance (Acc 10m ), Illinois agility test (IAT), and 300-yard shuttle run test (ShR 300y ) without load, with a 5-kg load (handgun, spare empty magazine, baton, and handcuffs), and with a 10-kg load (loaded vest). The effects of different load conditions were investigated for both sexes using a repeated-measure analysis of variance and Cohen's effect size. Female students carried relatively heavier loads and were slower than male students in Acc 10m , IAT, and ShR 300y . Load significantly (p , 0.001) impaired the performance in Acc 10m , IAT, and ShR 300y in both sexes, but the effects were greater (p , 0.001) among female police students. A load as little as 5 kg was sufficient to reduce Acc 10m , IAT, and ShR 300y among police students. Increasing the load to 10 kg (i.e., wearing the body armor) did not significantly affect Acc 10m and IAT among male students, but it did affect Acc 10m and IAT among female students. Sprint endurance (i.e., ShR 300y ) was strongly affected by both loads regardless of sex. Strength and conditioning programs should focus on strength, power, and anaerobic endurance to improve performance while carrying occupational loads. Occasional training with loads similar to those at work may further ensure that officers are fit for purpose.