Accuracy of a raw saliva-based COVID-19 RT-LAMP diagnostic assay

Cabral, Kátia Maria dos Santos; Baptista, Ramon Cid Gismonti; Castineiras, Terezinha Marta Pereira Pinto; Tanuri, Amilcar; Carneiro, Fabiana Avila; Almeida, Marcius da Silva; Montero-Lomeli, Monica

doi:10.1016/j.bjid.2023.102790

The Brazilian Journal of Infectious Diseases

2023

DOI: 10.1016/j.bjid.2023.102790

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Accuracy of a raw saliva-based COVID-19 RT-LAMP diagnostic assay

Kátia Maria dos Santos Cabral,

Ramon Cid Gismonti Baptista,

Terezinha Marta Pereira Pinto Castineiras

et al.

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2024

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References 33 publications

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Challenges in the Diagnosis of SARS-CoV-2 Infection in the Nervous System

Da Silva,

Cabral-Castro,

Gonçalves

et al. 2024

Viruses

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Neurological involvement has been widely reported in SARS-CoV-2 infection. However, viral identification in the cerebrospinal fluid (CSF) is rarely found. The aim of this study is to evaluate the accuracy of virological and immunological biomarkers in CSF for the diagnosis of neuroCOVID-19. We analyzed 69 CSF samples from patients with neurological manifestations: 14 with suspected/confirmed COVID-19, with 5 additional serial CSF samples (group A), and as a control, 50 non-COVID-19 cases (group B—26 with other neuroinflammatory diseases; group C—24 with non-inflammatory diseases). Real-time reverse-transcription polymerase chain reaction (real-time RT-PCR) was used to determine SARS-CoV-2, and specific IgG, IgM, neopterin, and protein 10 induced by gamma interferon (CXCL-10) were evaluated in the CSF samples. No samples were amplified for SARS-CoV-2 by real-time RT-PCR. The sensitivity levels of anti-SARS-CoV-2 IgG and IgM were 50% and 14.28%, respectively, with 100% specificity for both tests. CXCL-10 showed high sensitivity (95.83%) and specificity (95.83%) for detection of neuroinflammation. Serial CSF analysis showed an association between the neuroinflammatory biomarkers and outcome (death and hospital discharge) in two cases (meningoencephalitis and rhombencephalitis). The detection of SARS-CoV-2 RNA and specific immunoglobulins in the CSF can be used for neuroCOVID-19 confirmation. Additionally, CXCL-10 in the CSF may contribute to the diagnosis and monitoring of neuroCOVID-19.

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Challenges in the Diagnosis of SARS-CoV-2 Infection in the Nervous System

Da Silva,

Cabral-Castro,

Gonçalves

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

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Accuracy of a raw saliva-based COVID-19 RT-LAMP diagnostic assay

Cited by 1 publication

References 33 publications

Challenges in the Diagnosis of SARS-CoV-2 Infection in the Nervous System

Challenges in the Diagnosis of SARS-CoV-2 Infection in the Nervous System

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