Accuracy and reproducibility of coronary flow rate assessment by real-time contrast echocardiography: In vitro and in vivo studies

Lalitte, Stéphane; Masugata, Hisashi; Peters, Barry; Togni, Mario; Strachan, Monet; Yao, Biguang; Kwan, Oi Ling; DeMaria, Anthony N.

doi:10.1067/mje.2001.112908

Cited by 42 publications

(35 citation statements)

References 6 publications

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“…Neither closed-chest MCE nor end-systolic triggering acquisition was validated with the established technique of microspheres; however, real-time acquisition is an accepted technique for MBF estimation. 44,45 Finally, we did not quantify absolute MBF. Recently, Vogel et al 14 demonstrated that normalizing A␤ obtained in the myocardial walls by the LV cavity signal intensity provides absolute measurements of MBF in humans.…”

Section: Raher Et Al Murine Contrast Echocardiographymentioning

confidence: 99%

In Vivo Characterization of Murine Myocardial Perfusion With Myocardial Contrast Echocardiography

et al. 2007

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Background-The ability to noninvasively evaluate murine myocardial blood flow (MBF) in vivo would provide an important tool for cardiovascular research. Myocardial contrast echocardiography (MCE) has been used to measure MBF; however, it has not been validated in mice. This study assesses whether MCE can evaluate MBF at rest and after vasodilation and measure the maximal augmentation (coronary reserve) of MBF in mice. Wild-type (WT) and nitric oxide synthase 3 (NOS3)-deficient (NOS3 Ϫ/Ϫ ) mice were studied. Methods and Results-MCE was performed at baseline and after intravenous infusion of acetylcholine or adenosine.Definity contrast agent was infused, and parasternal views were acquired in real-time mode. Replenishment curves of myocardial contrast were obtained, and rates of signal rise (␤) and plateau intensity (A) were calculated. MBF estimated by the product of A and ␤ (A␤) was compared with that measured with fluorescent microspheres. MCE analysis was feasible in 98% (52/53) of mice. MBF measured by microspheres increased with adenosine and correlated closely with A␤. There was no difference in MCE-derived MBF between WT and NOS3 Ϫ/Ϫ mice at rest. Adenosine infusion increased MBF by 3.0Ϯ0.6-fold in NOS3 Ϫ/Ϫ mice and 2.5Ϯ0.3-fold in WT (Pϭ0.58 between genotypes). Acetylcholine induced an increase of 2.4Ϯ0.2-fold in MBF in WT mice but did not increase MBF in NOS3 Ϫ/Ϫ mice (PϽ0.0005 versus WT). Conclusions-MBF, coronary reserve, and vasodilator responses can be evaluated accurately in the intact mouse by MCE.This method demonstrated a preserved coronary response to adenosine but an impaired acetylcholine-induced vasodilation in NOS3 Ϫ/Ϫ mice compared with WT mice.

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Section: Raher Et Al Murine Contrast Echocardiographymentioning

confidence: 99%

In Vivo Characterization of Murine Myocardial Perfusion With Myocardial Contrast Echocardiography

et al. 2007

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“…The signals from the microbubbles in the blood flow are therefore enhanced dramatically [19]. Thus, blood flowing with a very low velocity, such as in the microcirculation of the testis, can be detected, and the slightest change in blood perfusion in the contralateral testis can be quantitatively measured with the TICs [20].…”

Section: Groupmentioning

confidence: 99%

Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion

Chen

Zhan²,

Zhang³

et al. 2009

Asian J Androl

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The changes of blood perfusion of contralateral testis after unilateral testicular torsion remain controversial. In this study, 28 New Zealand white male rabbits were randomly divided into five groups. Group A (n = 8), the control group, underwent a sham operation on the unilateral testis without inducing testicular torsion. In groups B, C, and D (n = 5 each), unilateral testicular torsion was induced, and, after 3, 6 or 24 h, respectively, detorsion was performed. In group E (n = 5), permanent unilateral testicular torsion was applied. Contrast-enhanced ultrasound was used to observe the blood perfusion of the contralateral testis at the following stages: pre-torsion (preopration), immediately post-torsion (postopration), pre-detorsion, immediately post-detorsion, and late-stage post-detorsion (6-12 h post-detorsion in groups B-D) or at a similar time point (15-21 h post-torsion in group E). Time-intensity curves were generated, and the following parameters were derived and analyzed: arrival time, time to peak intensity, peak intensity, and half-time of the descending peak intensity. The analysis revealed that blood perfusion of the contralateral testis increased immediately after testicular torsion on the opposite side (P < 0.05), which increased with prolonged testicular torsion of the other testis. This research demonstrated that contrast-enhanced ultrasound was valuable in evaluating blood perfusion of the contralateral testis after unilateral testicular torsion.

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“…Qualitative assessment has not yet been standardized and the use of the same replenishment cut-off values for different left ventricular segments may be inappropriate. Lafitte et al [31] showed that myocardial contrast replenishment-derived quantitative parameters vary with angle and depth of beam path. Moreover, the comparison of myocardial contrast enhancement with the most opacified segment may be suboptimal due to artifacts [31].…”

Section: Study Limitationsmentioning

confidence: 99%

Impact of previous myocardial infarction on the incremental value of myocardial contrast to two-dimensional supine bicycle stress echocardiography in evaluation of coronary artery disease

Miszalski−Jamka

Kuntz‐Hehner

Schmidt

et al. 2009

International Journal of Cardiology

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Accuracy and reproducibility of coronary flow rate assessment by real-time contrast echocardiography: In vitro and in vivo studies

Cited by 42 publications

References 6 publications

In Vivo Characterization of Murine Myocardial Perfusion With Myocardial Contrast Echocardiography

In Vivo Characterization of Murine Myocardial Perfusion With Myocardial Contrast Echocardiography

Blood perfusion of the contralateral testis evaluated with contrast-enhanced ultrasound in rabbits with unilateral testicular torsion

Impact of previous myocardial infarction on the incremental value of myocardial contrast to two-dimensional supine bicycle stress echocardiography in evaluation of coronary artery disease

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