Accumulation of α-Keto Acids as Essential Components in Cyanide Assimilation by
            <i>Pseudomonas fluorescens</i>
            NCIMB 11764

Kunz, Daniel A.; Chen, Jui‐Lin; Pan, Guangliang

doi:10.1128/aem.65.4.1813-1813.1999

Cited by 7 publications

(8 citation statements)

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“…The phosphoribosylglycinamide formyltransferase activity of P. pseudoalcaligenes CECT5344 was determined, and we found that enzymatic activity was 41% higher in cyanide‐grown cells than in ammonia‐grown cells (data not shown). Although putative siderophore production in response to cyanide was initially reported by Chen and Kunz (1997) in Pseudomonas fluorescens , this iron‐chelating activity was proposed later to be due to the extracellular accumulation of α‐ketoacids which carry out the non‐enzymatic scavenging of cyanide in this bacterium (Kunz et al ., 1998). Thus, our finding that cyanide induces a putative formyltransferase probably involved in the synthesis of a siderophore points out again on the production of this type of specific iron chelators in response to cyanide.…”

Section: Resultsmentioning

confidence: 97%

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The cyanotrophic bacterium Pseudomonas pseudoalcaligenes CECT5344 responds to cyanide by defence mechanisms against iron deprivation, oxidative damage and nitrogen stress

Luque-Almagro

Huertas

Roldán

et al. 2007

Environmental Microbiology

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Two-dimensional (2-D) electrophoresis approach has been used to test protein expression changes in response to cyanide in the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344. This is a cyanide-assimilating strain which also grows in media containing cyanide-enriched effluent from the jewellery industry. The bacterium efficiently uses this residue as the sole nitrogen source for aerobic growth under alkaline pH with negligible nitrogen losses as HCN. Cell-free extracts isolated from P. pseudoalcaligenes grown with a jewellery residue, free cyanide or ammonium chloride as nitrogen source were subjected to 2-D electrophoresis and the spot patterns were examined to determine differential protein expression. Electrophoretic plates exhibiting an average of 1000 spots showed significant differences in the expression of about 44 proteins depending on the nitrogen source. Some of these protein spots were analysed by Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Characterization of five of these proteins reveals that cyanide shock induces proteins related to iron acquisition, regulation of nitrogen assimilation pathways and oxidative stress repairing and protection.

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Section: Resultsmentioning

confidence: 97%

“…Besides, bacteria and fungi have developed metabolic pathways to degrade cyanide by a number of oxidative, reductive and hydrolytic pathways, as well as through substitution/addition reactions involving synthesis and subsequent hydrolysis of nitriles (Ebbs, 2004) and cyanohydrines (Kunz et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

The cyanotrophic bacterium Pseudomonas pseudoalcaligenes CECT5344 responds to cyanide by defence mechanisms against iron deprivation, oxidative damage and nitrogen stress

Luque-Almagro

Huertas

Roldán

et al. 2007

Environmental Microbiology

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“…Along these lines, P. aeruginosa has been reported to have a detoxification mechanism that involves the excretion of α‐ketoglutarate, which will react spontaneously with cyanide to produce cyanohydrin (Von Tigerstrom and Campbell, 1966; Blumer and Haas, 2000). More recent studies have demonstrated that another Pseudomonad, Pseudomonas fluorescens , can use this cyanohydrin product as a source of nitrogen (Kunz et al. , 1992; 1998).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa RoxR, a response regulator related to Rhodobacter sphaeroides PrrA, activates expression of the cyanide‐insensitive terminal oxidase

Comolli

Donohue

2002

Molecular Microbiology

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Summary The facultative anaerobe Pseudomonas aeruginosa has multiple aerobic electron transport pathways, one of which is terminated by a cyanide‐insensitive oxidase (CIO). This study characterizes a P. aeruginosa two‐component system that regulates CIO production. The response regulator of this system (RoxR) has significant amino acid sequence similarity to PrrA of Rhodobacter sphaeroides and related proteins in other α‐proteobacteria. In heterologous complementation analysis, R. sphaeroides PrrA rescued the growth defect of a P. aeruginosa mutant lacking RoxR, and RoxR enabled photosynthetic growth of an R. sphaeroides PrrA mutant. Also, RoxR could substitute for PrrA in activating transcription in vitro, demonstrating that these proteins are functional homologues. P. aeruginosa strains lacking RoxR or the sensor kinase (RoxS) were more sensitive than wild type to the respiratory inhibitors cyanide and azide. The phenotypes of these mutant strains correlated with reduced cyanide‐insensitive O2 utilization and less cyanide‐dependent expression of the locus encoding the CIO (cioAB). The ability of purified RoxR to bind to the cioAB promoter region also suggests that this protein acts directly to regulate cioAB transcription. Therefore, RoxR appears to play a role in regulating the transcription of loci for P. aeruginosa energy‐generating enzymes similar to that of its homologues in α‐proteobacteria.

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“…Stress predominates among all the factors that enhance the concentrations of phytochemicals in fruits and vegetables. This makes sense when it is considered that all stress types (biotic/abiotic) are conducive to oxidative stress in plants [59], and oxidative signaling controls synthesis and accumulation of secondary metabolites [60]. Plants produce phenolic compounds as a defensive mechanism to biotic/abiotic stresses [61].…”

Section: Results Of the Analysis Of Strawberry Plantsmentioning

confidence: 99%

Quality Parameter Levels of Strawberry Fruit in Response to Different Sound Waves at 1000 Hz with Different dB Values (95, 100, 105 dB)

Özkurt

Altuntas

2018

Agronomy

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All living organisms perceive mechanical signals, regardless of their taxonomic classifications or life habits. Because of their immobility, plants are influenced by a variety of environmental stresses, such as mechanical stress, during their growth and development. Plants develop physiological behaviors to adapt to their environment for long-term development and evolution. Sound-induced stress—an abiotic stress factor—is an example of mechanical stress and is caused by sound waves generated by different sources. This stress has a negative effect on the development and growth of plants. The strawberry plants evaluated in this study were exposed to three different sound intensity levels (95, 100, 105 dB) at a constant frequency of 1000 Hz. In strawberry plants, stress induced by sound waves is thought to trigger increased production of secondary metabolites as a defense mechanism. To determine the effect of sound applications, the fresh and dry weights of the roots and shoots were measured in strawberry plants, and the pH, total soluble solids (Brix), titratable acidity, vitamin C, total sugar, total acid, and total phenols were analyzed in the fruits. Results show that the sound stress, which was produced at a constant frequency (1000 Hz) and different sound levels (95, 100, 105 dB), affects the growth parameters of the plant and several quality parameters of the fruit.

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Accumulation of α-Keto Acids as Essential Components in Cyanide Assimilation by Pseudomonas fluorescens NCIMB 11764

Cited by 7 publications

References 0 publications

The cyanotrophic bacterium Pseudomonas pseudoalcaligenes CECT5344 responds to cyanide by defence mechanisms against iron deprivation, oxidative damage and nitrogen stress

The cyanotrophic bacterium Pseudomonas pseudoalcaligenes CECT5344 responds to cyanide by defence mechanisms against iron deprivation, oxidative damage and nitrogen stress

Pseudomonas aeruginosa RoxR, a response regulator related to Rhodobacter sphaeroides PrrA, activates expression of the cyanide‐insensitive terminal oxidase

Quality Parameter Levels of Strawberry Fruit in Response to Different Sound Waves at 1000 Hz with Different dB Values (95, 100, 105 dB)

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