Accumulation of sugars and nucleosides in response to high salt and butanol stress in 1-butanol producing Synechococcus elongatus

Fathima, Artnice Mega; Laviña, Walter A.; Putri, Sastia Prama; Fukusaki, Eiichiro

doi:10.1016/j.jbiosc.2019.08.015

Cited by 4 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complete uptake of TAN was expected since N-NH 4 + is the preferred nitrogen form consumed by most microalgal and cyanobacterial species, followed by N-NO 3 − and finally N-NO 2 − . These trends have been reported in several studies that used diluted and undiluted digestate (Arashiro et al 2020 ; Fathima et al 2020 ). Although microalgae could be assumed to uptake most of NH 4 + , NO 3 − , NO 2 − , and PO 4 3− , however, the presence of nitrifying/denitrifying bacteria in the microalgae-bacterial consortium may have also contributed to the N and P consumption.…”

Section: Resultssupporting

confidence: 75%

Cultivation of carbohydrate-rich microalgae with great settling properties using cooling tower wastewater

Ortíz-Sánchez

Guillén-Garcés

Morales-Arrieta

et al. 2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

Wastewater treatment and simultaneous production of value-added products with microalgae represent a sustainable alternative. Industrial wastewater, characterized by high C/N molar ratios, can naturally improve the carbohydrate content in microalgae without the need for any external source of carbon while degrading the organic matter, macro-nutrients, and micro-nutrients. This study aimed to understand the treatment, reuse, and valorization mechanisms of real cooling tower wastewater (CWW) from a cement-processing industry mixed with domestic wastewater (DW) to produce microalgal biomass with potential for synthesis of biofuels or other value-added products. For this purpose, three photobioreactors with different hydraulic retention times (HRT) were inoculated simultaneously using the CWW-DW mixture. Macro- and micro-nutrient consumption and accumulation, organic matter removal, algae growth, and carbohydrate content were monitored for 55 days. High COD (> 80%) and macronutrient removals (> 80% of N and P) were achieved in all the photoreactors, with heavy metals below the limits established by local standards. The best results showed maximum algal growth of 1.02 g SSV L−1 and 54% carbohydrate accumulation with a C/N ratio of 31.24 mol mol−1. Additionally, the harvested biomass presented a high Ca and Si content, ranging from 11 to 26% and 2 to 4%, respectively. Remarkably, big flocs were produced during microalgae growth, which enhanced natural settling for easy biomass harvesting. Overall, this process represents a sustainable alternative for CWW treatment and valorization, as well as a green tool for generating carbohydrate-rich biomass with the potential to produce biofuels and fertilizers.

show abstract

Section: Resultssupporting

confidence: 75%

Cultivation of carbohydrate-rich microalgae with great settling properties using cooling tower wastewater

Ortíz-Sánchez

Guillén-Garcés

Morales-Arrieta

et al. 2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…As there is no difference in the photosystems between the two strains, a decrease in photosynthetic activity in response to BuOH production would also occur in the DC11 strain. Recently, a metabolomic study of the DC11 strain with BuOH stress treatment revealed an accumulation of nucleosides and depletion of XMP and ATP under BuOH stress conditions (Fathima et al, 2020). ATP depletion can be explained by a decrease in photosynthetic activity.…”

Section: Discussionmentioning

confidence: 99%

Effects of <i>n</i>-butanol production on metabolism and the photosystem in <i>Synechococcus elongatus</i> PCC 7942 based on metabolic flux and target proteome analyses

Wada

Uebayashi

Toya

et al. 2023

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Although n-butanol (BuOH) is an ideal fuel because of its superior physical properties, it has toxicity to microbes. Previously, a Synechococcus elongatus PCC 7942 derivative strain that produces BuOH from CO2 was developed by introducing six heterologous genes (BUOH-SE strain). To identify the bottleneck in BuOH production, the effects of BuOH production and its toxicity on central metabolism and the photosystem were investigated. Parental (WT) and BUOH-SE strains were cultured under autotrophic conditions. Consistent with the results of a previous study, BuOH production was observed only in the BUOH-SE strain. Isotopically non-stationary 13 C-metabolic flux analysis revealed that the CO2 fixation rate was much larger than the BuOH production rate in the BUOH-SE strain (1.70 vs 0.03 mmol gDCW -1 h -1 ), implying that the carbon flow for BuOH biosynthesis was less affected by the entire flux distribution. No large difference was observed in the flux of metabolism between the WT and BUOH-SE strains.Contrastingly, in the photosystem, the chlorophyll content and maximum O2 evolution rate per dry cell weight of the BUOH-SE strain were decreased to 81% and 43% of the WT strain, respectively. Target proteome analysis revealed that the amounts of some proteins related to antennae (ApcA, ApcD, ApcE, and CpcC), photosystem II (PsbB, PsbU, and Psb28-2), and cytochrome b6f complex (PetB and PetC) in photosystems decreased in the BUOH-SE strain. The activation of photosynthesis would be a novel approach for further enhancing BuOH production in S. elongatus PCC 7942.

show abstract

“…Using a high producer of 1-butanol S. elongatus DC11 strain, demonstrated a significant accumulation of sugars and nucleosides under salt and alcohol stress compared to the original construction of the strain background. The results obtained from this study may be useful for future strain enhancement strategies in S. elongatus , focusing specifically on the metabolic response of this strain to 1-butanol stress ( Fathima et al, 2020 ).…”

Section: Metabolomics-driven Strain Improvements In Different Organismsmentioning

confidence: 99%

Metabolomics-driven strain improvement: A mini review

Iman

Herawati²,

Fukusaki³

et al. 2022

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

In recent years, mass spectrometry-based metabolomics has been established as a powerful and versatile technique for studying cellular metabolism by comprehensive analysis of metabolites in the cell. Although there are many scientific reports on the use of metabolomics for the elucidation of mechanism and physiological changes occurring in the cell, there are surprisingly very few reports on its use for the identification of rate-limiting steps in a synthetic biological system that can lead to the actual improvement of the host organism. In this mini review, we discuss different strategies for improving strain performance using metabolomics data and compare the application of metabolomics-driven strain improvement techniques in different host microorganisms. Finally, we highlight several success stories on the use of metabolomics-driven strain improvement strategies, which led to significant bioproductivity improvements.

show abstract

Accumulation of sugars and nucleosides in response to high salt and butanol stress in 1-butanol producing Synechococcus elongatus

Cited by 4 publications

References 25 publications

Cultivation of carbohydrate-rich microalgae with great settling properties using cooling tower wastewater

Cultivation of carbohydrate-rich microalgae with great settling properties using cooling tower wastewater

Effects of <i>n</i>-butanol production on metabolism and the photosystem in <i>Synechococcus elongatus</i> PCC 7942 based on metabolic flux and target proteome analyses

Metabolomics-driven strain improvement: A mini review

Contact Info

Product

Resources

About