Accumulation of soluble sugars related to desiccation tolerance during rice (Oryza sativa L) seed development and maturation

Zhu, Cancan; Cheng, C. H.; Liu, X.; He, Jiaqi

doi:10.15258/sst.2007.35.3.12

Cited by 7 publications

(4 citation statements)

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“…Furthermore, raffinose is preferentially accumulated in the embryo while non-RFO carbohydrates such as sucrose and trehalose are more highly accumulated in the endosperm (Table 1 ). Interestingly, a colocalization between QTL of longevity and QTL controlling oligosaccharide contents (sucrose, raffinose, and stachyose) has been pointed out in Arabidopsis and rice (Bentsink et al, 2000 ; Zhu et al, 2007 ). Together with raffinose, trehalose can also protect proteins and membranes damages induced by desiccation (Fernandez et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

An Integrated “Multi-Omics” Comparison of Embryo and Endosperm Tissue-Specific Features and Their Impact on Rice Seed Quality

Galland

Lounifi

et al. 2017

Front. Plant Sci.

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Although rice is a key crop species, few studies have addressed both rice seed physiological and nutritional quality, especially at the tissue level. In this study, an exhaustive “multi-omics” dataset on the mature rice seed was obtained by combining transcriptomics, label-free shotgun proteomics and metabolomics from embryo and endosperm, independently. These high-throughput analyses provide a new insight on the tissue-specificity related to rice seed quality. Foremost, we pinpointed that extensive post-transcriptional regulations occur at the end of rice seed development such that the embryo proteome becomes much more diversified than the endosperm proteome. Secondly, we observed that survival in the dry state in each seed compartment depends on contrasted metabolic and enzymatic apparatus in the embryo and the endosperm, respectively. Thirdly, it was remarkable to identify two different sets of starch biosynthesis enzymes as well as seed storage proteins (glutelins) in both embryo and endosperm consistently with the supernumerary embryo hypothesis origin of the endosperm. The presence of a putative new glutelin with a possible embryonic favored abundance is described here for the first time. Finally, we quantified the rate of mRNA translation into proteins. Consistently, the embryonic panel of protein translation initiation factors is much more diverse than that of the endosperm. This work emphasizes the value of tissue-specificity-centered “multi-omics” study in the seed to highlight new features even from well-characterized pathways. It paves the way for future studies of critical genetic determinants of rice seed physiological and nutritional quality.

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Section: Discussionmentioning

confidence: 99%

An Integrated “Multi-Omics” Comparison of Embryo and Endosperm Tissue-Specific Features and Their Impact on Rice Seed Quality

Galland

Lounifi

et al. 2017

Front. Plant Sci.

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“…There is evidence that RFO (raffinose and stachyose) contribute to a stable glass formation and to the stabilization of membranes in extensively desiccated cells (Hoekstra et al, 2001). Acquisition of desiccation tolerance in seeds is tightly associated with RFO accumulation in several species (Zhu et al, 2007). Cold‐induced accumulation of RFO has been related to the capacity of alfalfa (Castonguay et al, 1995; Cunningham et al, 2003) and other species (Korn et al, 2010; Pennycooke et al, 2003; Peters and Keller, 2009) to withstand freeze‐induced desiccation.…”

Section: Discussionmentioning

confidence: 99%

Cold‐Induced Biochemical and Molecular Changes in Alfalfa Populations Selectively Improved for Freezing Tolerance

et al. 2011

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Synthetic populations obtained after successive cycles of recurrent selection for superior tolerance to freezing were used to probe the molecular bases of superior adaptation to cold in alfalfa {Medicago sativa L.). The initial genetic backgrounds and populations derived from the cultivars Apica and Evolution were assessed for their freezing tolerance and cold-induced molecular changes using plants acclimated to natural hardening conditions in an unheated greenhouse. Significant increase in freezing tolerance in response to recurrent selection was confirmed by the determination of the lethal temperature for 50% of the plants (LTjg). Improvement of freezing tolerance was associated with differences in cold-induced molecular changes. Starch reserves in crowns progressively declined during fall hardening with a more extensive mobilization observed in advanced cycles of selection. Concentrations of cryoprotective sugars and of the amino acids proline, asparagine, and arginlne increased during fall hardening and were significantly higher in recurrently selected populations than in the initial backgrounds. Transcript levels of two cold-regulated (COR) genes, cas15 and GaS, were strongly up-regulated during fall hardening and significantly increased in response to selection mostly in the Apica background. Our results provide evidence that recurrent selection for superior freezing tolerance in alfalfa induces marked changes in traits associated with the cold acclimation process.

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“…Soluble sugars are important energy storage substances and the main source of sweetness in plant seeds. Their content is closely related to the nutrition and quality of seeds (Zhu et al 2007 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Biofortified Rice Provides Rich Sakuranetin in Endosperm

Zhao,

Hu,

Zhou

et al. 2024

Rice

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Sakuranetin plays a key role as a phytoalexin in plant resistance to biotic and abiotic stresses, and possesses diverse health-promoting benefits. However, mature rice seeds do not contain detectable levels of sakuranetin. In the present study, a transgenic rice plant was developed in which the promoter of an endosperm-specific glutelin gene OsGluD-1 drives the expression of a specific enzyme naringenin 7-O-methyltransferase (NOMT) for sakuranetin biosynthesis. The presence of naringenin, which serves as the biosynthetic precursor of sakuranetin made this modification feasible in theory. Liquid chromatography tandem mass spectrometry (LC–MS/MS) validated that the seeds of transgenic rice accumulated remarkable sakuranetin at the mature stage, and higher at the filling stage. In addition, the panicle blast resistance of transgenic rice was significantly higher than that of the wild type. Specially, the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging was performed to detect the content and spatial distribution of sakuranetin and other nutritional metabolites in transgenic rice seeds. Notably, this genetic modification also did not change the nutritional and quality indicators such as soluble sugars, total amino acids, total flavonoids, amylose, total protein, and free amino acid content in rice. Meanwhile, the phenotypes of the transgenic plant during the whole growth and developmental periods and agricultural traits such as grain width, grain length, and 1000-grain weight exhibited no significant differences from the wild type. Collectively, the study provides a conceptual advance on cultivating sakuranetin-rich biofortified rice by metabolic engineering. This new breeding idea may not only enhance the disease resistance of cereal crop seeds but also improve the nutritional value of grains for human health benefits.

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Accumulation of soluble sugars related to desiccation tolerance during rice (Oryza sativa L) seed development and maturation

Cited by 7 publications

References 0 publications

An Integrated “Multi-Omics” Comparison of Embryo and Endosperm Tissue-Specific Features and Their Impact on Rice Seed Quality

An Integrated “Multi-Omics” Comparison of Embryo and Endosperm Tissue-Specific Features and Their Impact on Rice Seed Quality

Cold‐Induced Biochemical and Molecular Changes in Alfalfa Populations Selectively Improved for Freezing Tolerance

Biofortified Rice Provides Rich Sakuranetin in Endosperm

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