Accumulation of Poly(3-hydroxybutyrate) from Octanoate in Different Pseudomonas Belonging to the rRNA Homology Group I

Diard, Stéphane; Carlier, Jean‐Philippe; Ageron, Elisabeth; Grimont, Patrick A. D.; Langlois, Valérie; Guérin, Philippe; Bouvet, Odile

doi:10.1078/0723-2020-00114

Cited by 27 publications

(15 citation statements)

References 31 publications

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“…While mcl‐PHA accumulation is widely reported in pseudomonads, scl‐PHA production is not a common characteristic of Pseudomonas species (Diard et al ., 2002 ). P. oleovorans group including P. oleovorans , P. pseudoalcaligenes , Pseudomonas sp., as well as recently described P. extremoaustralis and Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

“…P. oleovorans group including P. oleovorans , P. pseudoalcaligenes , Pseudomonas sp., as well as recently described P. extremoaustralis and Pseudomonas sp. MPC6 are examples of Pseudomonas species that have the capacity to accumulate scl‐PHA when grown with sodium octanoate (Diard et al ., 2002 ; Lopez et al ., 2009 ; Catone et al ., 2014 ; Orellana‐Saez et al ., 2019 ). In P. extremoaustralis , the scl‐PHA, polyhydroxybutyrate (PHB) cluster phaRBAC is located in a genomic island of 32.3 kb containing 28 ORFs (Ayub et al ., 2007 ).…”

Section: Resultsmentioning

confidence: 99%

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Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Narancic

Salvador

Hughes

et al. 2021

Microbial Biotechnology

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The throwaway culture related to the single-use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET-derived terephthalic acid (TA) into PHA and performed an in-depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilize TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Narancic

Salvador

Hughes

et al. 2021

Microbial Biotechnology

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“…14‐3 showed high PHB production (80 wt %) in overnight cultures grown in accumulation medium containing sodium octanoate as carbon source (Table 2). PHB production from octanoate has been described in a few other Pseudomonas species (Diard et al , 2002). PHB accumulation in Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Impaired polyhydroxybutyrate biosynthesis from glucose inPseudomonassp. 14-3 is due to a defective Î²-ketothiolase gene

Ayub

Pettinari

Méndez

et al. 2006

FEMS Microbiology Letters

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Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a beta-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous beta-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its beta-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative beta-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids.

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“…[4]. PHB production has been reported in some Pseudomonas strains, such as those included in the “ P. oleovorans group” [5].…”

Section: Introductionmentioning

confidence: 99%

High Polyhydroxybutyrate Production in Pseudomonas extremaustralis Is Associated with Differential Expression of Horizontally Acquired and Core Genome Polyhydroxyalkanoate Synthase Genes

et al. 2014

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Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species. Previous studies with this strain demonstrated that PHB genes are located in a genomic island. In this work, the analysis of the genome of P. extremaustralis revealed the presence of another PHB cluster phbFPX, with high similarity to genes belonging to Burkholderiales, and also a cluster, phaC1ZC2D, coding for medium chain length PHA production (mclPHA). All mclPHA genes showed high similarity to genes from Pseudomonas species and interestingly, this cluster also showed a natural insertion of seven ORFs not related to mclPHA metabolism. Besides PHB, P. extremaustralis is able to produce mclPHA although in minor amounts. Complementation analysis demonstrated that both mclPHA synthases, PhaC1 and PhaC2, were functional. RT-qPCR analysis showed different levels of expression for the PHB synthase, phbC, and the mclPHA synthases. The expression level of phbC, was significantly higher than the obtained for phaC1 and phaC2, in late exponential phase cultures. The analysis of the proteins bound to the PHA granules showed the presence of PhbC and PhaC1, whilst PhaC2 could not be detected. In addition, two phasin like proteins (PhbP and PhaI) associated with the production of scl and mcl PHAs, respectively, were detected. The results of this work show the high efficiency of a foreign gene (phbC) in comparison with the mclPHA core genome genes (phaC1 and phaC2) indicating that the ability of P. extremaustralis to produce high amounts of PHB could be explained by the different expression levels of the genes encoding the scl and mcl PHA synthases.

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Accumulation of Poly(3-hydroxybutyrate) from Octanoate in Different Pseudomonas Belonging to the rRNA Homology Group I

Cited by 27 publications

References 31 publications

Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Impaired polyhydroxybutyrate biosynthesis from glucose inPseudomonassp. 14-3 is due to a defective Î²-ketothiolase gene

High Polyhydroxybutyrate Production in Pseudomonas extremaustralis Is Associated with Differential Expression of Horizontally Acquired and Core Genome Polyhydroxyalkanoate Synthase Genes

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