Accumulation of PHA and its copolyesters by Methylobacterium sp. KCTC 0048

Kang, Choong Kyung; Lee, Hyun Sook; Kim, Jung Ho

doi:10.1007/bf00129929

Cited by 22 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 Thus far, P3HP cannot be produced by any native bacterial strains. When some structurally-related carbon sources like 3HP, a,v-alkanediols, acrylate, g-butyrolactone or 1,3-propanediol were added, PHAs containing the 3HP monomers could be produced by some microbes like Alcaligenes eutrophus, 7 Alcaligenes latus, 8 Ralstonia eutropha, 9 Methylobacterium, 10 and engineered Escherichia coli. 11 However, the addition of these expensive precursors increased the production cost and restricted the further development.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂

et al. 2015

View full text Add to dashboard Cite

(2015) Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiellapneumoniae strain without vitamin B 12 , Bioengineered, 6:2, 77-81, DOI: 10.1080/21655979.2015 The present study examined the production of P3HP from glycerol in the recombinant Klebsiella pneumoniae strain, which naturally synthesizes vitamin B 12 . The genes glycerol dehydratase and its reactivation factor (dhaB123, gdrA, and gdrB from K. pneumoniae), aldehyde dehydrogenase (aldH from E. coli) were cloned and expressed in K. pneumoniae to produce 3-hydroxypropionate (3HP), with 2 genes (dhaT and yqhD) for biosynthesis of 1,3-propanediol were deleted. To obtain P3HP production, propionyl-CoA synthetase (prpE from E. coli) and polyhydroxyalkanoate synthase (phaC from Ralstonia eutropha) were introduced. Under the appropriate aeration condition, the cell yield and P3HP content were 0.24 g/L and 12.7% (wt/wt [cell dry weight]) respectively along with 2.03 g/L 3HP after 48 h cultivation. Although the yield is relatively low, this study shows the feasibility of producing P3HP in K. pneumoniae from glycerol without vitamin B 12 for the first time. The results also suggest that the aeration conditions should be optimized carefully for the efficient production of P3HP.

show abstract

Section: Introductionmentioning

confidence: 99%

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Higher fractions (4.3 and 10.5 mol%) of 3HP moieties were incorporated by Methylobacterium sp. KCTC 0048 when the strain was fed with 3HP (Table 3) (39). For a fully controlled 3HP-to-3-hydroxybutyrate (3HB) composition process, Azohydromonas lata (formerly Alcaligenes latus [40]) was cultivated in the presence of various amounts of 3HP and 3HB and accumulated poly(3HP-co-3HB)s with 3HP fractions of between 7 and 88 mol% (16).…”

Section: Hp-containing Copolymersmentioning

confidence: 99%

Poly(3-Hydroxypropionate): a Promising Alternative to Fossil Fuel-Based Materials

Andreeßen

Taylor

Steinbüchel

2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bPolyhydroxyalkanoates (PHAs) are storage compounds synthesized by numerous microorganisms and have attracted the interest of industry since they are biobased and biodegradable alternatives to fossil fuel-derived plastics. Among PHAs, poly(3-hydroxypropionate) [poly(3HP)] has outstanding material characteristics and exhibits a large variety of applications. As it is not brittle like, e.g., the best-studied PHA, poly(3-hydroxybutyrate) [poly(3HB)], it can be used as a plasticizer in blends to improve their properties. Furthermore, 3-hydroxypropionic acid (3HP) is considered likely to become one of the new industrial building blocks, and it can be obtained from poly(3HP) by simple hydrolysis. Unfortunately, no natural organism is known to accumulate poly(3HP) so far. Thus, several efforts have been made to engineer genetically modified organisms capable of synthesizing the homopolymer or copolymers containing 3HP. In this review, the achievements made so far in efforts to obtain biomass which has accumulated poly(3HP) or 3HP-containing copolymers, as well as the properties of these polyesters and their applications, are compiled and evaluated.

show abstract

“…Another attempt to assemble poly(3HB-co-3HP) was described by Kang et al (23). The authors cultivated Methylobacterium sp.…”

Section: Bacterial Synthesis Of Poly(3hb-co-3hp) Copolymersmentioning

confidence: 99%

Biosynthesis and Biodegradation of 3-Hydroxypropionate- Containing Polyesters

Andreeßen

Steinbüchel

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

3-Hydroxypropionate (3HP) is an important compound in the chemical industry, and the polymerized 3HPcan be used as a bioplastic. In this review, we focus on polyesters consisting of 3HP monomers, including the homopolyester poly(3-hydroxypropionate) and copolyesters poly(3-hydroxybutyrate-co-3-hydroxypropionate), poly(3-hydroxypropionate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate), poly(4-hydroxybutyrate-co-3-hydroxypropionate-co-lactate), and poly(3-hydroxybutyrate-co-3-hydroxypropionate-co-4-hydroxybutyrate-co-lactate). Homopolyesters like poly(3-hydroxybutyrate) are often highly crystalline and brittle, which limits some of their applications. The incorporation of 3HP monomers reduces the glass transition temperature, the crystallinity, and also, at up to 60 to 70 mol% 3HP, the melting point of the copolymer. This review provides a survey of the synthesis and physical properties of different polyesters containing 3HP.Bacterial polyhydroxyalkanoates (PHAs) are natural biodegradable thermoplastics produced by various microorganisms as intracellular energy and carbon storage compounds. PHAs have attracted increased attention as possible alternatives to petroleum-based polymers. They are biodegradable, insoluble in water, nontoxic, biocompatible, piezoelectric, thermoplastic, and/or elastomeric. These features make PHAs suitable for several applications in the packaging industry, medicine, pharmacy, agriculture, and food industry, as raw materials for the production of enantiomerically pure chemicals, and for the production of paints (2, 44). The best characterized PHA is poly(3-hydroxybutyrate) [poly(3HB)], which is synthesized by many bacteria (28,31,38,41). Unfortunately, poly(3HB) is a highly crystalline and brittle polymer with a low elongation-to-break factor, which has prevented its use in a wide range of applications. To obtain bacterial PHAs with improved physical and mechanical properties, previous studies have demonstrated the biosynthesis of copolyesters consisting of 3-hydroxybutyrate (3HB) and a second constituent. Pathways for the biosynthesis of such PHA copolyesters, like poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)], poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [poly(3HB-co-4HV)], and poly(3-hydroxybutyrate-co-3-hydroxypropionate)

show abstract

Accumulation of PHA and its copolyesters by Methylobacterium sp. KCTC 0048

Cited by 22 publications

References 11 publications

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂

Poly(3-Hydroxypropionate): a Promising Alternative to Fossil Fuel-Based Materials

Biosynthesis and Biodegradation of 3-Hydroxypropionate- Containing Polyesters

Contact Info

Product

Resources

About

Accumulation of PHA and its copolyesters by Methylobacterium sp. KCTC 0048

Cited by 22 publications

References 11 publications

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B12

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B12

Poly(3-Hydroxypropionate): a Promising Alternative to Fossil Fuel-Based Materials

Biosynthesis and Biodegradation of 3-Hydroxypropionate- Containing Polyesters

Contact Info

Product

Resources

About

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂

Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineeredKlebsiella pneumoniaestrain without vitamin B₁₂