Accumulation of miR-155 and BIC RNA in human B cell lymphomas

Abstract

We show that the microRNA miR-155 can be processed from sequences present in BIC RNA, a spliced and polyadenylated but non-protein-coding RNA that accumulates in lymphoma cells. The precursor of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated BIC RNA, which is primarily cytoplasmic. By using a sensitive and quantitative assay, we find that clinical isolates of several types of B cell lymphomas, including diffuse large B cell lymphoma (

Altered expression of MicroRNA in synovial fibroblasts and synovial tissue in rheumatoid arthritis

“…Mature miR-155 is generated from a precursor form known as BIC RNA, encoded by the bic gene and known to be linked with lymphoma (30). Under normal conditions, miR-155 has been described to be expressed by human T cells, B cells, monocytes, and endothelial cells (29,(31)(32)(33).…”

New meaning in the message: Noncoding RNAs and their role in retinal development

“…Finally, pre-miRNAs are cleaved in the cytoplasm by Dicer. In the relatively few cases where primiRNAs are easily detected, such as BIC/miR-155 in immune cells, the primiRNA is far less abundant than the corresponding mature miRNA (Eis et al, 2005;Kluiver et al, 2005).…”

Implications of micro-RNA profiling for cancer diagnosis

“…Although expression differences may not necessarily reflect causal events of tumorigenesis, such changes may, nevertheless, regulate genes important in tumor pathogenesis and may be useful for classifying tumors and predicting their outcomes. Examples of such gene expression alterations in miRNAs have been detected in CLL (Calin et al, 2002), colorectal neoplasia (Michael et al, 2003;Cummins et al, 2006), pituitary adenomas (Bottoni et al, 2005), lung cancer (Takamizawa et al, 2004;Johnson et al, 2005), Burkitt's lymphoma (Metzler et al, 2004), B-cell lymphoma (Eis et al, 2005;He et al, 2005b;Kluiver et al, 2005), breast cancer , glioblastoma (Chan et al, 2005;Ciafre et al, 2005) and papillary thyroid cancer (PTC) (He et al, 2005a).…”

“…For breast cancers, miRNA profiles have been reported to distinguish breast tumors from normal breast epithelium, and have been correlated with specific breast cancer pathologic features such as estrogen and progesterone receptor status, tumor stage, vascular invasion and proliferation index . A single miRNA, B-cell integration cluster (BIC)/miR-155, appears to be dramatically overexpressed in several types of B-cell lymphomas, including a subset of diffuse large B-cell lymphomas (DLBCLs) (Eis et al, 2005;Kluiver et al, 2005). DLBCL samples with an activated B-cell phenotype had high levels of BIC/miR-155, whereas those with the germinal center phenotype did not.…”