Accumulation of medium chain fatty acids in Nannochloropsis oceanica by heterologous expression of Cuphea palustris thioesterase FatB1

Südfeld, Christian; Kiyani, Aamna; Buckens, Hortense; Hubáček, Michal; Wijffels, René H.; Barbosa, María J.; D’Adamo, Sarah

doi:10.1016/j.algal.2022.102665

Cited by 4 publications

(4 citation statements)

References 66 publications

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“…Tailor-designed thioesterases are the critical chain control step in medium-chain fatty acids synthesis. Other engineered thioesterases such as CpFatB1 have been reported in other microbial cell factories (Hernández Lozada et al, 2020;Krishnan et al, 2020;Südfeld et al, 2022).…”

Section: Fatty Acid Supply Modulementioning

confidence: 99%

“…Only ‘TesA was evaluated in P. putida . Other tailor‐optimized thioesterases such as ‘TesA RD‐2 and CpFatB1* have been used to produce medium‐chain fatty acids in other microbes such as E. coli and microalgae (Cho et al., 2020 ; Hernández Lozada et al., 2020 ; Lennen & Pfleger, 2013 ; Südfeld et al., 2022 ). Similarly, these thioesterases can be used to further improve the fatty acid supply in P. putida .…”

Section: Future Perspectivementioning

confidence: 99%

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Pseudomonas putida as a platform for medium‐chain length α,ω‐diol production: Opportunities and challenges

Lu,

Wijffels,

Martins dos Santos

et al. 2024

Microbial Biotechnology

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Medium‐chain‐length α,ω‐diols (mcl‐diols) play an important role in polymer production, traditionally depending on energy‐intensive chemical processes. Microbial cell factories offer an alternative, but conventional strains like Escherichia coli and Saccharomyces cerevisiae face challenges in mcl‐diol production due to the toxicity of intermediates such as alcohols and acids. Metabolic engineering and synthetic biology enable the engineering of non‐model strains for such purposes with P. putida emerging as a promising microbial platform. This study reviews the advancement in diol production using P. putida and proposes a four‐module approach for the sustainable production of diols. Despite progress, challenges persist, and this study discusses current obstacles and future opportunities for leveraging P. putida as a microbial cell factory for mcl‐diol production. Furthermore, this study highlights the potential of using P. putida as an efficient chassis for diol synthesis.

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Section: Fatty Acid Supply Modulementioning

confidence: 99%

Section: Future Perspectivementioning

confidence: 99%

Pseudomonas putida as a platform for medium‐chain length α,ω‐diol production: Opportunities and challenges

Lu,

Wijffels,

Martins dos Santos

et al. 2024

Microbial Biotechnology

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“…oceanica has also been engineered to produce tailored lipids, such as medium-chain fatty acids. For example, overexpression of acyl-ACP thioesterase from Cuphea palustris resulted in a small amount (2–4%) of C8:0 and C10:0 fatty acids in TAG, while introducing engineered C. palustris thioesterase with a preference to C12:0 led to ∼2–6% of C12:0 production in N.…”

Section: Metabolic Engineering For Lipid Production In N Oceanicamentioning

confidence: 99%

“…21 N. oceanica has also been engineered to produce tailored lipids, such as medium-chain fatty acids. For example, overexpression of acyl-ACP thioesterase from Cuphea palustris resulted in a small amount (2−4%) of C8:0 and C10:0 fatty acids in TAG, 60 while introducing engineered C. palustris thioesterase with a preference to C12:0 led to ∼2−6% of C12:0 production in N. oceanica TAG. 61 In addition to metabolic engineering, other approaches, such as synthetic co-cultures with oleaginous fungi, have been explored to improve oil production of N. oceanica.…”

Section: Production In N Oceanicamentioning

confidence: 99%

Biochemistry and Biotechnology of Lipid Accumulation in the Microalga Nannochloropsis oceanica

2022

J. Agric. Food Chem.

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Oils are among the most important agricultural commodities and have wide applications in food/nutrition, biofuels, and oleochemicals. The oleaginous microalga Nannochloropsis oceanica can produce large amounts of oils and the high-value ω-3 eicosapentaenoic acid, which represents a promising resource for oil production targeting biodiesel, nutraceutical, and aquaculture industries. In recent years, with the availability of omics databases and the development of genetic tools, N. oceanica has been extensively investigated as a model photosynthetic organism for studying lipid metabolism and as a green cellular factory to produce lipids for industrial applications. This review summarizes the current knowledge on the lipid composition and biosynthetic pathways of N. oceanica and reviews the recent advances in metabolic engineering of lipid production in this microalga.

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Hypes, hopes, and the way forward for microalgal biotechnology

Barbosa

Janssen

Südfeld

et al. 2023

Trends in Biotechnology

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Accumulation of medium chain fatty acids in Nannochloropsis oceanica by heterologous expression of Cuphea palustris thioesterase FatB1

Cited by 4 publications

References 66 publications

Pseudomonas putida as a platform for medium‐chain length α,ω‐diol production: Opportunities and challenges

Pseudomonas putida as a platform for medium‐chain length α,ω‐diol production: Opportunities and challenges

Biochemistry and Biotechnology of Lipid Accumulation in the Microalga Nannochloropsis oceanica

Hypes, hopes, and the way forward for microalgal biotechnology

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