Accumulation of macular xanthophylls in unsaturated membrane domains

Wiśniewska, Anna; Subczynski, Witold K.

doi:10.1016/j.freeradbiomed.2006.01.016

Cited by 48 publications

(54 citation statements)

References 39 publications

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“…However, the overall concentration of the cholesterol employed in nanodiscs can be smaller to ensure native-like membrane confinement in the model system. This feature is also relevant, because preparation of nanodiscs requires homogenous suspension of lipids and detergent in buffer, and cholesterol is known to form detergent resistant fractions with saturated lipids [40,41] thus preparation of nanodisc with high concentration of cholesterol can prove to be unattainable in standard systems. The solution to this problem can be the detergent free systems where up to 10 mol% of cholesterol was successfully incorporated into the DMPC based nanodiscs [4].…”

Section: Discussionmentioning

confidence: 99%

Comparative EPR studies on lipid bilayer properties in nanodiscs and liposomes

Stępień

Polit

Wiśniewska-Becker

2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Studies of the membrane proteins suggest their close interaction with the lipid surroundings. Membrane proteins and their activities are affected by the composition and structure of the lipid bilayer. therefore adequate surroundings for studied protein are crucial for the model membrane to ensure its biological relevance. In recent years nanodiscs which are small fragments of lipid bilayer stabilised by derivatives of apolipoprotein, called membrane scaffold protein ( MSP), have been established as alternative tool in structural and functional studies of membrane proteins. In this study, the influence MSP of different length on structure and dynamics of DMPC and POPC bilayer was investigated and compared to bilayer present in liposomes. EPR spectroscopy technique using different PC-based spin probes was employed to show cholesterol-like organising effect of MSPs on lipid bilayer, thus giving a better insight into the nanodiscs model membrane structure, and its possible implications in the research of membrane protein applications.

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Section: Discussionmentioning

confidence: 99%

Comparative EPR studies on lipid bilayer properties in nanodiscs and liposomes

Stępień

Polit

Wiśniewska-Becker

2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…According to their structure, carotenoids may be distributed in diferent ways into the cell compartments. In particular, they can be found within the inner section of the lipid bilayer of cell membranes, only if they are strict hydrocarbons like β-carotene or lycopene, or they can protrude into an aqueous environment from the membrane surface with a hydrophilic portion if they contain oxygen atoms, which confer them a more polar structure [14,15]. Xanthophylls, such as lutein, fucoxanthin, neoxanthin, and xanthophyll cycle pigments, are among these more hydrophilic carotenoids.…”

Section: Carotenoids: Function and Distribution In Photosynthetic Cellsmentioning

confidence: 99%

Synthesis of Antioxidant Carotenoids in Microalgae in Response to Physiological Stress

Faraloni¹,

Torzillo²

2017

Carotenoids

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Carotenoids act as potential antioxidants, quenching energy of excited singlet oxygen and scavenging free radicals. Among microalgae, Haematococcus, Chlamydomonas, Chlorella, Dunaliella and diatoms and dinolagellates, such as Phaeodactylum and Isochrysis, are able to synthesize large amount of carotenoids. The main function of carotenoids consists in absorbing light to perform photosynthesis, and some of them are constitutively present in the cells (primary carotenoids). The main primary carotenoids usually found are neoxanthin, violaxanthin, lutein, and β-carotene. To preserve cells from oxidative damage, their production may be increased, while other carotenoids may be synthesized de novo. In particular, under stress conditions such as high light exposure, nutrient starvation, change in oxygen partial pressure, and high or low temperatures, microalgal metabolism is altered and photosynthetic activity may be reduced. In these conditions, photosynthetic electrons transport is reduced, and the intracellular reduction level increase may be associated with the formation of free radicals and species containing singlet oxygen. In order to prevent damage from photooxidation, microalgae are able to adopt strategies to contrast these dangerous oxidant molecules. One of the most active mechanisms is to synthesize large amount of carotenoids, which can act as antioxidants.

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“…Recent interest in zeaxanthin is due to the discovery that it represents a major carotenoid in the retinal pigment of the eye (Krinsky, Landrom, & Bone, 2003). According to Wisniewska and Subczynski (2006), the presence of zeaxanthin and/or lutein in the diet may be beneficial for reducing the incidence of the two common eye diseases of ageing, age-related macular degeneration and cataracts formation.…”

Section: Xanthophyllsmentioning

confidence: 99%

“…For example, carotenes are the sources of vitamin A (Olson, 1994). Lutein and zeaxanthin are important factors for human vision (Wisniewska & Subczynski, 2006). Carotenoids and chlorophylls have an important role in the prevention of various diseases associated with oxidative stress, such as cancer, cardiovascular diseases and other chronic diseases (Sangeetha & Baskaran, 2010).…”

Section: Introductionmentioning

confidence: 99%