Accumulation of Iron Oxide Nanoparticles by Cultured Brain Astrocytes

Geppert, Mark; Hohnholt, Michaela C.; Gaetjen, Linda; Grunwald, Ingo; Bäumer, Marcus; Dringen, Ralf

doi:10.1166/jbn.2009.1033

Cited by 55 publications

(72 citation statements)

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“…Magnetic Fe-NP were synthesized by using a wet chemical method as described previously [14] using the method initially described by Bee et al [16] . The stock solution of Fe-NP in water (80 Â 10 À3 M total iron concentration) was diluted with an equal volume of 80 Â 10 À3 M freshly prepared aqueous tri-sodium citrate, resulting in an aqueous stable dispersion of citrate-coated Fe-NP that contained 40 Â 10 À3 M of total iron.…”

Section: Synthesis and Coating Of Fe-npmentioning

confidence: 99%

“…[14] Cells were exposed to citrate-coated Fe-NP in 1 mL incubation buffer (20 Â 10 À3 M HEPES, 145 Â 10 À3 M NaCl, 1.8 Â 10 À3 M CaCl 2 , 5.4 Â 10 À3 M KCl, 1 Â 10 À3 M MgCl 2 , 5Â 10 À3 M glucose, 10 Â 10 À3 M trisodium citrate) that had been adjusted to pH 7.4 at the desired temperature. After the given incubation periods, the cells were washed twice with 1 mL of ice-cold phosphate-buffered saline (PBS: 10 Â 10 À3 M potassium phosphate buffer pH 7.4, containing 150 Â 10 À3 M NaCl) and dry cells were stored frozen until quantification of their iron and protein contents.…”

Section: Cell Culture and Experimental Incubationsmentioning

confidence: 99%

“…Iron contents in media and cells were quantified by the ferrozine-based colorimetric assay [22] in the modification described previously. [14,19] Visualization of Cellular Iron and Fe-NP by Perls' Staining and TEM Cellular iron was visualized by the cytochemical Perls' staining [23] as described previously. [14,19] For TEM analysis, cells grown on Aclarfilm (Ted Pella, Redding, USA) in wells of 24-well dishes were incubated with 1000 Â 10 À6 M citratecoated Fe-NP, washed with ice-cold PBS and prefixed with 2.5% w/v glutaraldehyde in 0.1 M sodium cacodylate buffer pH 7.3 for 60 min.…”

Section: Determination Of Cell Viability and Quantification Of Cellulmentioning

confidence: 99%

“…cultured astrocytes appears not to be acutely toxic to these cells. [7,14,15] These cells accumulate iron even better from Fe-NP than from identical amounts (100 Â 10 À6 M total iron) of low molecular weight iron sources. [14] Here, we report that cultured astrocytes accumulate iron efficiently from citrate-coated Fe-NP in a time-, concentration-, and temperature dependent manner.…”

mentioning

confidence: 99%

“…[7,14,15] These cells accumulate iron even better from Fe-NP than from identical amounts (100 Â 10 À6 M total iron) of low molecular weight iron sources. [14] Here, we report that cultured astrocytes accumulate iron efficiently from citrate-coated Fe-NP in a time-, concentration-, and temperature dependent manner. Presence of cellular iron after exposure of astrocytes to Fe-NP was demonstrated by iron quantification, by cytochemical Perls' staining for iron as well as by transmission electron microscopy (TEM) of Fe-NP in the cells.…”

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confidence: 99%

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Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

et al. 2010

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Magnetic iron oxide nanoparticles (Fe‐NP) are considered for various applications in the brain. However, little is known so far on the uptake and the metabolism of such nanoparticles in brain cells. Since astrocytes are strategically localized between capillaries and neurons, astrocytes are of particular interest concerning uptake and fate of nanoparticles in the brain. Using astrocyte‐rich primary cultures as model system we have investigated the accumulation of citrate‐coated Fe‐NP by astrocytes. Viable cultured astrocytes accumulate iron from citrate‐coated Fe‐NP in a time‐, concentration‐, and temperature‐dependent manner. The cellular iron content determined after 4 h of incubation increases proportional to the concentration of Fe‐NP, if the particles were applied in concentrations of up to 1000 × 10−6 M of total iron. The iron accumulation from 500 or 1000 × 10−6 M iron as Fe‐NP is significantly slowed by lowering the incubation temperature from 37 to 4 °C. Transmission electron microscopy of the cells revealed that most of the cellular Fe‐NP are present in intracellular vesicles. These data demonstrate that astrocytes accumulate efficiently citrate‐coated Fe‐NP, most likely by an endocytotic pathway.

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Section: Synthesis and Coating Of Fe-npmentioning

confidence: 99%

Section: Cell Culture and Experimental Incubationsmentioning

confidence: 99%

Section: Determination Of Cell Viability and Quantification Of Cellulmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

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Fibrous Materials for Antimicrobial Applications

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Bifunctional Submicron Colloidosomes Coassembled from Fluorescent and Superparamagnetic Nanoparticles

et al. 2014

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Colloidosomes are microcapsules consisting of nanoparticle shells. These microcarriers can be self‐assembled from a wide range of colloidal particles with selective chemical, physical, and morphological properties and show promise for application in the field of theranostic nanomedicine. Previous studies have mainly focused on fairly large colloidosomes (>1 μm) based on a single kind of particle; however, the intrinsic building‐block nature of this microcarrier has not been exploited so far for the introduction of tailored functionality at the nanoscale. We report a synthetic route based on interfacial shear rheology studies that allows the simultaneous incorporation of different nanoparticles with distinct physical properties, that is, superparamagnetic iron oxide and fluorescent silica nanoparticles, in a single submicron colloidosome. These tailor‐made microcapsules can potentially be used in various biomedical applications, including magnetic hyperthermia, magnetic particle imaging, drug targeting, and bioimaging.

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Accumulation of Iron Oxide Nanoparticles by Cultured Brain Astrocytes

Cited by 55 publications

References 0 publications

Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

Advanced Biomaterials Accumulation of Citrate‐Coated Magnetic Iron Oxide Nanoparticles by Cultured Brain Astrocytes

Fibrous Materials for Antimicrobial Applications

Bifunctional Submicron Colloidosomes Coassembled from Fluorescent and Superparamagnetic Nanoparticles

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