Accumulation of high OPDA level correlates with reduced ROS and elevated GSH benefiting white cell survival in variegated leaves

Sun, Ying-Hsuan; Hung, Chiu-Yueh; Qiu, Jie; Chen, Jianjun; Kittur, Farooqahmed S.; Oldham, Carla E.; Henny, Richard J.; Burkey, Kent O.; Fan, Longjiang; Xie, Jiahua

doi:10.1038/srep44158

Cited by 20 publications

(21 citation statements)

References 87 publications

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“…Our study found that chloroplasts have no intact lamellar structure in albino tissues, and that photosynthesis was suppressed. Similar results were also observed in other albino or variegated plants (Sun et al, 2017;Shih et al, 2019;Li et al, 2020). As shown in Figure 4A, four major photosynthetic complexes in plants, including PSII, PSI, the cytochrome b 6 /f (cytb 6 /f ) complex, and plastid ATP synthase, are located in the chloroplast thylakoid membrane (Rochaix, 2004).…”

Section: Photomorphogenesis Affects Chloroplast Developmentsupporting

confidence: 86%

Disruption of Photomorphogenesis Leads to Abnormal Chloroplast Development and Leaf Variegation in Camellia sinensis

Gao

Zhang

et al. 2021

Front. Plant Sci.

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Camellia sinensis cv. ‘Yanlingyinbiancha’ is a leaf-variegated mutant with stable genetic traits. The current study aimed to reveal the differences between its albino and green tissues, and the molecular mechanism underlying the variegation. Anatomic analysis showed the chloroplasts of albino tissues to have no intact lamellar structure. Photosynthetic pigment in albino tissues was significantly lower than that in green tissues, whereas all catechin components were more abundant in the former. Transcriptome analysis revealed most differentially expressed genes involved in the biosynthesis of photosynthetic pigment, photosynthesis, and energy metabolism to be downregulated in albino tissues while most of those participating in flavonoid metabolism were upregulated. In addition, it was found cryptochrome 1 (CRY1) and phytochrome B (PHYB) genes that encode blue and red light photoreceptors to be downregulated. These photoreceptors mediate chloroplast protein gene expression, chloroplast protein import and photosynthetic pigment biosynthesis. Simultaneously, SUS gene, which was upregulated in albino tissues, encodes sucrose synthase considered a biochemical marker for sink strength. Collectively, we arrived to the following conclusions: (1) repression of the biosynthesis of photosynthetic pigment causes albinism; (2) destruction of photoreceptors in albino tissues suppresses photomorphogenesis, leading to abnormal chloroplast development; (3) albino tissues receive sucrose from the green tissues and decompose their own storage substances to obtain the energy needed for survival; and (4) UV-B signal and brassinosteroids promote flavonoid biosynthesis.

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Section: Photomorphogenesis Affects Chloroplast Developmentsupporting

confidence: 86%

Disruption of Photomorphogenesis Leads to Abnormal Chloroplast Development and Leaf Variegation in Camellia sinensis

Gao

Zhang

et al. 2021

Front. Plant Sci.

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“…The contents of GSH and ROS were responsible for in vivo oxidative damage . Following the BIIC treatment, the serum GSH content (Figure C) was reduced, while the ROS level (Figure D) was elevated remarkably, indicating that the oxidative damage was increased upon the BIIC stimulation.…”

Section: Resultsmentioning

confidence: 96%

Protective Effect of Tangeretin and 5-Hydroxy-6,7,8,3′,4′-Pentamethoxyflavone on Collagen-Induced Arthritis by Inhibiting Autophagy via Activation of the ROS-AKT/mTOR Signaling Pathway

Yang

Xia

Zhong

et al. 2020

J. Agric. Food Chem.

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Rheumatoid arthritis (RA) is an autoimmune disease characterized by long duration and repeated relapse. This study explored the preventive effect of tangeretin (TAN) and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (5-HPMF) on RA, and the underlying molecular mechanism based on a rat model stimulated by bovine type II collagen (BIIC). After the intervention of TAN or 5-HPMF (TAN/5-HPMF) for 5 weeks, the RA lesions and autophagy levels of the synovial tissue were significantly reduced, and the ROS content and HO-1 expression level were down-regulated simultaneously. The relative expression levels of p-AKT and p-mTOR were down-regulated after TAN/5-HPMF feeding. Meanwhile, the relative expression level of p62 increased by more than two-fold for TAN/5-HPMF treated rats at 200 mg/kg BW comparing with those in BIIC group. Results of immunofluorescence staining and Western blotting further confirmed that TAN/5-HPMF treatment reduced BIIC-induced conversion from LC3I to LC3II. Observations under transmission electron microscope also demonstrated that the autophagy level was reduced upon TAN/5-HPMF intervention. Collectively, these results revealed that TAN and 5-HPMF prevented the pathological process of BIIC-stimulated arthritis through inhibiting the autophagy of synovial cells, achieved via the ROS-AKT/mTOR signal axis. Thus, our findings confirmed the protective potential of TAN and 5-HPMF for RA disease.

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“…In the Arabidopsis white-green variegated mutant immutans ( im ), an inhibition of carotenoids formation was observed [38]. Similarly, the white section in leaf of variegated Epipremnum aureum contains 10-fold less carotenoids than the green section [39]. In Cyclamen purpurascens , the light green leaf stripes were found with reduced carotenoids and chlorophyll contents [40].…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Leaf Variegation in Pittosporum tobira under Low Temperature: Insights into the Physio-Biochemical and Molecular Mechanisms

Zhang

Song

et al. 2019

IJMS

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Leaf variegation has been demonstrated to have adaptive functions such as cold tolerance. Pittosporum tobira is an ornamental plant with natural leaf variegated cultivars grown in temperate regions. Herein, we investigated the role of leaf variegation in low temperature responses by comparing variegated “Variegatum” and non-variegated “Green Pittosporum” cultivars. We found that leaf variegation is associated with impaired chloroplast development in the yellow sector, reduced chlorophyll content, strong accumulation of carotenoids and high levels of ROS. However, the photosynthetic efficiency was not obviously impaired in the variegated leaves. Also, leaf variegation plays low temperature protective function since “Variegatum” displayed strong and efficient ROS-scavenging enzymatic systems to buffer cold (10 °C)-induced damages. Transcriptome analysis under cold conditions revealed 309 differentially expressed genes between both cultivars. Distinctly, the strong cold response observed in “Variegatum” was essentially attributed to the up-regulation of HSP70/90 genes involved in cellular homeostasis; up-regulation of POD genes responsible for cell detoxification and up-regulation of FAD2 genes and subsequent down-regulation of GDSL genes leading to high accumulation of polyunsaturated fatty acids for cell membrane fluidity. Overall, our results indicated that leaf variegation is associated with changes in physiological, biochemical and molecular components playing low temperature protective function in P. tobira.

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Accumulation of high OPDA level correlates with reduced ROS and elevated GSH benefiting white cell survival in variegated leaves

Cited by 20 publications

References 87 publications

Disruption of Photomorphogenesis Leads to Abnormal Chloroplast Development and Leaf Variegation in Camellia sinensis

Disruption of Photomorphogenesis Leads to Abnormal Chloroplast Development and Leaf Variegation in Camellia sinensis

Protective Effect of Tangeretin and 5-Hydroxy-6,7,8,3′,4′-Pentamethoxyflavone on Collagen-Induced Arthritis by Inhibiting Autophagy via Activation of the ROS-AKT/mTOR Signaling Pathway

Protective Role of Leaf Variegation in Pittosporum tobira under Low Temperature: Insights into the Physio-Biochemical and Molecular Mechanisms

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